Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica

Repair of airway epithelium after viral infection involves migration of epithelial cells to cover injured, denuded areas. We determined whether viral infection reduces the capability of bronchial epithelial cells to migrate and to attach to extracellular matrix proteins. Inoculation of bovine bronchial epithelial cells in vitro with bovine herpesvirus-1 reduced their ability to migrate in two different assays of cell migration. When attachment assays were performed, fewer cells attached to both control wells and matrix protein-precoated wells, suggesting that general mechanisms of adherence to substrates were altered by viral infection. Focal contact points of epithelial cells with the underlying matrix were evaluated with epifluorescence microscopy and monoclonal antibodies to vinculin and alpha v, an integrin chain. Disruption of focal contact points was seen early after infection and was prevented by an inhibitor of viral DNA polymerase, phosphonoacetic acid. Cycloheximide did not cause similar disruptions of focal contacts at early time points. Viral infection thus has marked effects on the interactions of bronchial epithelial cells with extracellular matrix and the organization of matrix to cytoskeleton links. The effects appear to be dependent in part on viral replication in the cells and are not simply due to reductions in host cell protein synthesis.

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In Vitro Transformation of Human Bronchial Epithelial Cells by Diesel Exhaust Particles: Gene Expression Profiling and Early Toxic Responses

Toxicological Sciences ◽

10.1093/toxsci/kfy183 ◽

2018 ◽

Cited By ~ 6

Author(s):

Iselin Rynning ◽

Jiri Neca ◽

Kristyna Vrbova ◽

Helena Libalova ◽

Pavel Rossner ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Diesel Exhaust ◽

Expression Profiling ◽

Diesel Exhaust Particles ◽

Human Bronchial Epithelial Cells ◽

Bronchial Epithelial ◽

Exhaust Particles ◽

Toxic Responses

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In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000272013 ◽

2016 ◽

Vol 82 (0) ◽

Author(s):

Roseli Fernandes Gonçalves ◽

Rodrigo Martins Soares ◽

Cássia Maria Barroso Orlandi ◽

Leonardo José Richtzenhain ◽

José Antonio Visintin ◽

...

Keyword(s):

Epithelial Cells ◽

Cytopathic Effect ◽

Bovine Herpesvirus ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

Bovine Herpesvirus 1 ◽

Mdbk Cells ◽

Herpesvirus 1 ◽

Polymerase Chain

The aims of this study were to assess in vitro if bovine oocytes and oviductal epithelial cells from slaughterhouses for in vitro fertilization use may be infected with bovine herpesvirus 1; to analyze whether the treatment with trypsin according to the International Embryo Transfer Society guideline is efficient to inactivate the bovine herpesvirus 1; to morphologically study the virus-oocyte interaction through optical microscopy. In this study, Madin Darby Bovine Kidney (MDBK) cells that were co-cultured with oocytes matured in vitro and exposed to bovine herpesvirus 1 showed a cytopathic effect. The nested polymerase chain reaction for the supernatant was positive for the bovine herpesvirus 1, thus suggesting that the cytopathic effect observed in the MDBK monolayer was seen due to virus replication and not because of any culture toxicity. It was also observed cytopathic effect and positive nested polymerase chain reaction in MDBK cells co-cultured with in vitro maturated oocytes free of virus, but that were co-cultured in uterine epithelial cells pre-infected with bovine herpesvirus 1 and washed or not with trypsin, demonstrating an oocyte contamination by the virus. When trypsin-washing efficacy was evaluated, we could observe that the trypsin treatment was not able to eliminate the bovine herpesvirus 1 of the oocytes, and it was not observed any morphological difference in the infected oocytes.

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In vitro cadmium effects on ECM gene expression in human bronchial epithelial cells

Cytokine ◽

10.1016/j.cyto.2014.12.002 ◽

2015 ◽

Vol 72 (1) ◽

pp. 9-16 ◽

Cited By ~ 16

Author(s):

Tiziano Baroni ◽

Cinzia Lilli ◽

Catia Bellucci ◽

Giovanni Luca ◽

Francesca Mancuso ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Bronchial Epithelial Cells ◽

Human Bronchial Epithelial Cells ◽

Bronchial Epithelial

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Gene expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli and Staphylococcus aureus in vitro

Veterinary Research ◽

10.1186/s13567-015-0178-z ◽

2015 ◽

Vol 46 (1) ◽

Cited By ~ 12

Author(s):

Alexandra Jaeger ◽

Danilo Bardehle ◽

Michael Oster ◽

Juliane Günther ◽

Eduard Muráni ◽

...

Keyword(s):

Gene Expression ◽

Escherichia Coli ◽

Staphylococcus Aureus ◽

Epithelial Cells ◽

Gene Expression Profiling ◽

Expression Profiling ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

And Staphylococcus Aureus

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Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells

Scientific Reports ◽

10.1038/s41598-021-81843-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Fabio Arruda-Silva ◽

Paolo Bellavite ◽

Marta Marzotto

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Differentially Expressed Genes ◽

Bronchial Epithelial Cells ◽

Differentially Expressed ◽

Biological Functions ◽

Bronchial Epithelial ◽

Cell Functions ◽

Drosera Rotundifolia

AbstractDrosera rotundifolia has been traditionally used for the treatment of respiratory diseases in phytotherapy and homeopathy. The mechanisms of action recognized so far are linked to the known effects of specific components, such as flavonoids, but are not completely understood. In this study, the biological functions of D. rotundifolia were explored in vitro following the treatment of bronchial epithelial cells, which are the potential targets of the pharmacological effects of the herbal medicine. To do so, the whole plant ethanolic extract was 1000-fold diluted in water (D. rotundifolia 3×) and added to a 16HBE human cell line culture for 3 h or 6 h. The effects on gene expression of the treatments and corresponding controls were then investigated by RNA sequencing. The differentially expressed genes were validated through RT-qPCR, and the enriched biological functions involved in the effects of treatment were investigated. D. rotundifolia 3× did not impair cell viability and was shown to be a stimulant of cell functions by regulating the expression of dozens of genes after 3 h, and the effects were amplified after 6 h of treatment. The main differentially expressed genes encoded ligands of epithelial growth factor receptor, proteins involved in xenobiotic detoxification and cytokines, suggesting that D. rotundifolia 3× could stimulate self-repair systems, which are impaired in airway diseases. Furthermore, D. rotundifolia 3× acts on a complex and multifaceted set of genes and may potentially affect different layers of the bronchial mucosa.

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