Haemonchus contortus infection induces a variable immune response in resistant and susceptible Pelibuey sheep

2021 ◽  
pp. 110218
Author(s):  
Alvar Alonzo Cruz-Tamayo ◽  
Ma. Eugenia López-Arellano ◽  
Roberto González-Garduño ◽  
Glafiro Torres-Hernández ◽  
Alfonso de la Mora-Valle ◽  
...  
Keyword(s):  
Immune Response ◽  
Haemonchus Contortus ◽  
Pelibuey Sheep

scholarly journals Immune response to Haemonchus contortus infection in susceptible (INRA 401) and resistant (Barbados Black Belly) breeds of lambs

2007 ◽  
Vol 29 (8) ◽  
pp. 415-424 ◽  
Author(s):  
G. TEREFE ◽  
C. LACROUX ◽  
O. ANDREOLETTI ◽  
C. GRISEZ ◽  
F. PREVOT ◽  
...  
Keyword(s):  
Immune Response ◽  
Haemonchus Contortus

Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 351-357 ◽  
Author(s):  
H. D. F. H. Schallig ◽  
M. A. W. van Leeuwen ◽  
W. M. L. Hendrikx
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Haemonchus Contortus ◽  
Lymphocyte Proliferation ◽  
The Other ◽  
Proliferation Index ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Molecular Weights ◽  
Secretory Products

SUMMARYThe excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgGI levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material- based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections.

scholarly journals Predicting the effects of parasite co-infection across species boundaries

2018 ◽  
Vol 285 (1874) ◽  
pp. 20172610 ◽  
Author(s):  
Joanne Lello ◽  
Susan J. McClure ◽  
Kerri Tyrrell ◽  
Mark E. Viney
Keyword(s):  
Immune Response ◽  
Haemonchus Contortus ◽  
Parasite Species ◽  
Nematode Species ◽  
Blood Feeding ◽  
Species Boundaries ◽  
Parasite Transmission ◽  
Parasite Control ◽  
Host System ◽  
Specific Outcome

It is normal for hosts to be co-infected by parasites. Interactions among co-infecting species can have profound consequences, including changing parasite transmission dynamics, altering disease severity and confounding attempts at parasite control. Despite the importance of co-infection, there is currently no way to predict how different parasite species may interact with one another, nor the consequences of those interactions. Here, we demonstrate a method that enables such prediction by identifying two nematode parasite groups based on taxonomy and characteristics of the parasitological niche. From an understanding of the interactions between the two defined groups in one host system (wild rabbits), we predict how two different nematode species, from the same defined groups, will interact in co-infections in a different host system (sheep), and then we test this experimentally. We show that, as predicted, in co-infections, the blood-feeding nematode Haemonchus contortus suppresses aspects of the sheep immune response, thereby facilitating the establishment and/or survival of the nematode Trichostrongylus colubriformis ; and that the T. colubriformis -induced immune response negatively affects H. contortus . This work is, to our knowledge, the first to use empirical data from one host system to successfully predict the specific outcome of a different co-infection in a second host species. The study therefore takes the first step in defining a practical framework for predicting interspecific parasite interactions in other animal systems.

scholarly journals Nanoparticles (PLGA and Chitosan)-Entrapped ADP-Ribosylation Factor 1 of Haemonchus contortus Enhances the Immune Responses in ICR Mice

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 726
Author(s):  
Muhammad Waqqas Hasan ◽  
Muhammad Haseeb ◽  
Muhammad Ehsan ◽  
Javaid Ali Gadahi ◽  
Muhammad Ali-ul-Husnain Naqvi ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Haemonchus Contortus ◽  
Lymphocyte Proliferation ◽  
Mononuclear Cells ◽  
Secretory Proteins ◽  
Adp Ribosylation ◽  
Icr Mice ◽  
Cytokine Analysis ◽  
Adp Ribosylation Factor

ADP-ribosylation factor 1 (HcARF1) is one of the Haemonchus contortus (H. contortus) excretory/secretory proteins involved in modulating the immune response of goat peripheral blood mononuclear cells (PBMC). Here, we evaluated the immunogenic potential of recombinant HcARF1 (rHcARF1) against H. contortus infection in Institute of Cancer Research (ICR) mice. Briefly, rHcARF1 was entrapped in poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NP) and injected into mice as a vaccine. Fifty-six ICR mice were assigned randomly into seven groups, with eight animals in each group, and they were vaccinated subcutaneously. At the end of the experiment (14th day), the blood and the spleen were collected from euthanized mice to detect lymphocyte proliferation, cytokine analysis, and the production of antigen-specific antibodies. Scanning electron microscope was used to determine the size, morphology, and zeta potential of nanoparticles. Flow cytometry was performed, which presented the increase percentages of CD4+ T cells (CD3e+CD4+), CD8+ T cells (CD3e+CD8+) and dendritic cells (CD11c+CD83+, CD11c+CD86+) in mice vaccinated with rHcARF1+PLGA NP. Immunoassay analysis show raised humoral (Immunoglobulin (Ig)G1, IgG2a, IgM) and cell-mediated immune response (Interleukin (IL)-4, IL-12, and IL-17, and Interferon (IFN)-γ) induced by rHcARF1+PLGA NP. Experimental groups that were treated with the antigen-loaded NP yield higher lymphocyte proliferation than the control groups. Based on these results, we could propose that the rHcARF1 encapsulated in NP could stimulate a strong immune response in mice rather than administering alone against the infection of H. contortus.

scholarly journals Association analysis of immune response loci related to Haemonchus contortus exposure in sheep and goats using a targeted approach

2019 ◽  
Vol 228 ◽  
pp. 109-119
Author(s):  
Zaira M. Estrada-Reyes ◽  
Yoko Tsukahara ◽  
Arthur L. Goetsch ◽  
Terry A. Gipson ◽  
Tilahun Sahlu ◽  
...  
Keyword(s):  
Immune Response ◽  
Association Analysis ◽  
Haemonchus Contortus ◽  
Sheep And Goats ◽  
Targeted Approach

Genetic resistance to helminths. The influence of breed and haemoglobin type on the response of sheep to primary infections withHaemonchus contortus

Parasitology ◽  
1978 ◽  
Vol 77 (2) ◽  
pp. 161-175 ◽  
Author(s):  
K. I. Altaif ◽  
J. D. Dargie
Keyword(s):  
Immune Response ◽  
Haemonchus Contortus ◽  
Genetic Resistance ◽  
Physiological Factors ◽  
Larval Stages ◽  
Haemoglobin Type ◽  
Breed Differences ◽  
Metabolic Activities ◽  
Severity Of The Disease ◽  
Pathogenic Effects

SummaryThe possible existence of strain and breed differences in the response of sheep to primary infections withHaemonchus contortuswas examined by comparing the establishment and pathogenic effects of the parasite in Scottish Blackface and Finn Dorset sheep grouped according to haemoglobin type and infected with 7–10000 3rd-stage larvae. Homozygous haemoglobin A-type sheep of both breeds had lower worm burdens and faecal egg outputs, and suffered less severe clinical and pathophysiological disturbances than animals homozygous for haemoglobin B. In addition, Scottish Blackface sheep displayed similar advantages over Finn Dorsets with the same haemoglobin type and variations in the severity of the disease as judged by a variety of pathophysiological indices correlated closely with parasite numbers. It was therefore concluded that genetic resistance operated primarily against worm establishment and that, barring the unlikely involvement of non-specific physiological factors, this was controlled by the immune response elicited. The nature of this response is unknown, but appeared to operate only against the larval stages, since the size and metabolic activities of the surviving worms were similar in all animals. In a subsequent experiment, designed to examine the response of Scottish Blackface sheep to heavy infection (45000 larvae), there was no correlation between worm establishment and haemoglobin type. This was possibly due to a delayed immune response arising from exposure to excessive amounts of antigen.

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