Characterization of Bovine papillomavirus 28 (BPV28) and a novel genotype BPV29 associated with vulval papillomas in cattle

Camel papillomatosis has been described previously, but the genome of the suspected papillomavirus (PV) has not been identified. An outbreak of papillomatosis occurred in a dromedary farm of 55 animals in Sudan during August 2009. The disease was only present in young animals aged about 3–7 months, of which 44 % (11/25) were affected with lesions, mainly on the lips and lower jaw. This study reports for the first time the complete genomes of Camelus dromedarius papillomavirus types 1 (CdPV1) and 2 (CdPV2), isolated from a cauliflower-like nodule and a round oval raised nodule, respectively. Pairwise comparisons of their L1 nucleotide sequences revealed 69.2 % identity, and phylogenetic analyses suggested that these two PV types are grouped within the genus Deltapapillomavirus. Both viruses were isolated from fibropapillomas, although no putative E5 proteins homologous to that of bovine papillomavirus type 1 were identified. The genetic information will be useful for evolutionary studies of the family Papillomaviridae, as well as for the development of diagnostic methods for surveillance of the disease in dromedaries.

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Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1.

The EMBO Journal ◽

10.1002/j.1460-2075.1991.tb05010.x ◽

1991 ◽

Vol 10 (13) ◽

pp. 4321-4329 ◽

Cited By ~ 148

Author(s):

M. Ustav ◽

E. Ustav ◽

P. Szymanski ◽

A. Stenlund

Keyword(s):

Bovine Papillomavirus ◽

Origin Of Replication ◽

Viral Origin ◽

Origin Recognition

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First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Parasite ◽

10.1051/parasite/2020019 ◽

2020 ◽

Vol 27 ◽

pp. 21 ◽

Cited By ~ 1

Author(s):

Jingsong Wang ◽

Chaochao Lv ◽

Diandian Zhao ◽

Runan Zhu ◽

Chen Li ◽

...

Keyword(s):

Rattus Norvegicus ◽

Guinea Pigs ◽

Enterocytozoon Bieneusi ◽

Its Sequence ◽

The Novel ◽

Microsporidian Parasite ◽

Obligate Intracellular ◽

Reference Sequences ◽

Novel Genotype

Enterocytozoon bieneusi, an obligate intracellular microsporidian parasite, can infect humans and a wide variety of animals worldwide. However, information on the prevalence and molecular characterization of E. bieneusi in pet rats and guinea pigs is lacking. In this study, 325 fecal samples were collected from 152 pet fancy rats and 173 pet guinea pigs purchased from pet shops in Henan and Shandong provinces. The prevalence of E. bieneusi was 11.2% (17/152) in pet fancy rats and 20.2% (35/173) in pet guinea pigs. Genotypes D (n = 12), Peru11 (n = 3), S7 (n = 1) and SCC-2 (n = 1) were identified in pet fancy rats, and genotype S7 (n = 30) and a novel genotype PGP (n = 5) were identified in pet guinea pigs. The ITS sequence and its phylogenetic analysis showed that the novel genotype PGP was distinctly different; it exhibited less than 50% similarity to the reference sequences, and did not cluster with any of the known E. bieneusi genotype groups, forming a unique branch between groups 6 and 7. These data suggest that this is a new E. bieneusi genotype group. This is the first report of E. bieneusi infection in pet fancy rats and pet guinea pigs worldwide. The identification of zoonotic genotypes D, Peru11, and S7 suggests that pet fancy rats and guinea pigs can be potential sources of human microsporidiosis.

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Characterization of a novel genotype of iGiardia/i from a Quenda (iIsoodon obesulus/i) from Western Australia.

iGiardia/i: the cosmopolitan parasite ◽

10.1079/9780851996127.0287 ◽

2017 ◽

pp. 287-291

Author(s):

P. J. Adams ◽

R. C. A. Thompson

Keyword(s):

Western Australia ◽

Novel Genotype

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Expression andIn SilicoAnalysis of the Recombinant Bovine Papillomavirus E6 Protein as a Model for Viral Oncoproteins Studies

BioMed Research International ◽

10.1155/2013/421398 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

J. Mazzuchelli-de-Souza ◽

R. F. Carvalho ◽

R. M. Ruiz ◽

T. C. Melo ◽

R. P. Araldi ◽

...

Keyword(s):

Recombinant Proteins ◽

In Silico ◽

Cell Transformation ◽

Bovine Papillomavirus ◽

Viral Oncoprotein ◽

Viral Oncoproteins ◽

Biotechnological Products ◽

Clone And Express ◽

E6 Protein

Bovine papillomaviruses (BPVs) are recognized as the causal agents of economical relevant diseases in cattle, associated with the development of tumors in skin and mucosa. The oncogenesis process is mainly associated with different viral oncoprotein expressions, which are involved in cell transformation. The expression and characterization of recombinant viral oncoproteins represent an attractive strategy to obtain biotechnological products as antibodies and potential vaccines, Thus, the aim of this work was to clone and express the BPV-1 and BPV-2 E6 recombinant proteins and performin silicoanalysis in order to develop a strategy for the systematic study of other papillomaviruses oncoproteins. The results demonstrated that BPV-1 and BPV-2 E6 recombinant proteins were expressed and purified from bacterial system as well as itsin silicoanalysis was performed in order to explore and predict biological characteristics of these proteins.

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