Effects of single or trickle Haemonchus contortus experimental infection on digestibility and host responses of naïve Creole kids reared indoor

Considering the importance of Haemonchus contortus infection in herds along with parasitic resistance, the goal of this study was to evaluate the influence of the administration of adjuvants alone or in combination with anthelmintics for the treatment of H. contortus, in experimentally infected sheep. Thirty sheep of the Texel breed of both genders, raised in a herd located in the subtropical region of Brazil, were used in this experiment. Experimental infection with H. contortus was performed in sheep, and the infected sheep were then separated into groups for the administration of antiparasitic and immunostimulant drugs. The results obtained from the excretion of eggs per gram of feces and the count of parasites during necropsy affirm that the use of adjuvants in combination with anthelmintics is associated with higher efficacy of treatment, lower rate of reinfection, and retardation in the development of anthelmintic drug resistance by H. contortus. Based on these results, we can conclude that the combination of anthelmintics and immunostimulants may favor potential anthelmintic treatments for H. contortus.

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Experimental infection by Haemonchus contortus in lambs: influence of disease on purine levels in serum

Parasitology ◽

10.1017/s0031182013002370 ◽

2014 ◽

Vol 141 (7) ◽

pp. 898-903 ◽

Cited By ~ 9

Author(s):

LUCAS T. GRESSLER ◽

ALEKSANDRO S. DA SILVA ◽

CAMILA B. OLIVEIRA ◽

ANDRESSA S. SCHAFER ◽

ADELINA R. AIRES ◽

...

Keyword(s):

High Pressure ◽

Uric Acid ◽

Liquid Chromatography ◽

High Pressure Liquid Chromatography ◽

Experimental Infection ◽

Post Infection ◽

Haemonchus Contortus ◽

Inflammatory Process ◽

Group A ◽

Group B

SUMMARYThe aim of this study was to evaluate the purine levels of lambs experimentally infected with Haemonchus contortus. A total of 12 healthy lambs were divided into two groups, composed of 6 animals each: Group A represented the healthy animals (uninfected), while in Group B the animals were infected with 15 000 larvae of H. contortus. Blood was drawn on days 15, 45 and 75 post-infection (PI) in order to perform the purine analysis (ATP, ADP, AMP, adenosine, inosine, hypoxanthine, xanthine and uric acid) by high pressure liquid chromatography (HPLC) in serum. On day 15 PI a significant (P<0·05) increase in the levels of ATP and inosine was observed in the infected animals, unlike the levels of ADP, adenosine, xanthine and uric acid which were reduced. On day 45 PI a significant (P<0·05) increase in the ATP and xanthine levels in infected animals was observed, contrasting with reduced levels of ADP and uric acid. Finally, on day 75 PI an increase occurred in the levels of ATP, adenosine and hypoxanthine in infected lambs, concomitant with a reduction in the levels of ADP and uric acid (P<0·05). These changes in purine levels may influence the inflammatory process and the pathological events.

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