Sarcocystis neurona infection in gamma interferon gene knockout (KO) mice: Comparative infectivity of sporocysts in two strains of KO mice, effect of trypsin digestion on merozoite viability, and infectivity of bradyzoites to KO mice and cell culture

2013 ◽  
Vol 196 (1-2) ◽  
pp. 212-215 ◽  
Author(s):  
J.P. Dubey ◽  
N. Sundar ◽  
O.C.H. Kwok ◽  
W.J.A. Saville
Keyword(s):  
Cell Culture ◽  
Gene Knockout ◽  
Gamma Interferon ◽  
Trypsin Digestion ◽  
Sarcocystis Neurona ◽  
Interferon Gene

Molecular characterization and development ofSarcocystis speerisarcocysts in gamma interferon gene knockout mice

Parasitology ◽  
2015 ◽  
Vol 142 (13) ◽  
pp. 1555-1562 ◽  
Author(s):  
J. P. DUBEY ◽  
S. K. VERMA ◽  
D. DUNAMS ◽  
R. CALERO-BERNAL ◽  
B. M. ROSENTHAL
Keyword(s):  
Intermediate Host ◽  
Definitive Host ◽  
Gene Knockout ◽  
Gamma Interferon ◽  
Didelphis Virginiana ◽  
Post Inoculation ◽  
28S Rrna ◽  
The North ◽  
Gene Knockout Mice ◽  
Interferon Gene

SUMMARYThe North American opossum (Didelphis virginiana) is the definitive host for at least three named species ofSarcocystis: Sarcocystis falcatula, Sarcocystis neuronaandSarcocystis speeri.The South American opossums (Didelphis albiventris, Didelphis marsupialisandDidelphis aurita) are definitive hosts forS. falcatulaandS. lindsayi. The sporocysts of theseSarcocystisspecies are similar morphologically. They are also not easily distinguished genetically because of the difficulties of DNA extraction from sporocysts and availability of distinguishing genetic markers. Some of these species can be distinguished by bioassay;S. neuronaandS. speeriare infective to gamma interferon gene knockout (KO) mice, but not to budgerigars (Melopsittacus undulatus); whereasS. falcatulaandS. lindsayiare infective to budgerigars but not to KO mice. The natural intermediate host ofS. speeriis unknown. In the present study, development of sarcocysts ofS. speeriin the KO mice is described. Sarcocysts were first seen at 12 days post-inoculation (p.i.), and they became macroscopic (up to 4 mm long) by 25 days p.i. The structure of the sarcocyst wall did not change from the time bradyzoites had formed at 50–220 days p.i. Sarcocysts contained unique villar protrusions, ‘type 38’. The polymerase chain reaction amplifications and sequences analysis of three nuclear loci (18S rRNA, 28S rRNAandITS1) and two mitochondrial loci (cox1andcytb) ofS. speeriisolate from an Argentinean opossum (D. albiventris) confirmed its membership among species ofSarcocystisand indicated an especially close relationship to another parasite in this genus that employs opossums as its definitive host,S. neurona.These results should be useful in finding natural intermediate host ofS. speeri.

scholarly journals Inactivation of Cryptosporidium parvum Oocysts in Fresh Apple Cider by UV Irradiation

2002 ◽  
Vol 68 (8) ◽  
pp. 4168-4172 ◽  
Author(s):  
D. E. Hanes ◽  
R. W. Worobo ◽  
P. A. Orlandi ◽  
D. H. Burr ◽  
M. D. Miliotis ◽  
...  
Keyword(s):  
Mouse Models ◽  
Uv Irradiation ◽  
Cryptosporidium Parvum ◽  
Gene Knockout ◽  
Gamma Interferon ◽  
Apple Cider ◽  
Cryptosporidium Parvum Oocysts ◽  
Interferon Gene

ABSTRACT This study evaluated the efficacy of UV irradiation on the inactivation of Cryptosporidium parvum oocysts in fresh apple cider. Cider was inoculated with oocysts and exposed to 14.32 mJ of UV irradiation/cm2. Oocyst viability was assessed with the gamma interferon gene knockout (GKO) mouse and infant BALB/cByJ mouse models. All GKO mice challenged with UV-treated cider demonstrated no morbidity or mortality, and infant BALB/c mice challenged with treated cider were negative for the presence of C. parvum. In contrast, the GKO mice challenged with non-UV-treated inoculated cider died and the parasite was detected in the ileums of all challenged infant mice. This study shows that UV irradiation can be used to inactivate C. parvum in fresh apple cider.

GAMMA-INTERFERON GENE EXPRESSION IN HUMAN RENAL ALLOGRAFT FINE-NEEDLE ASPIRATES

1994 ◽  
Vol 57 (4) ◽  
pp. 498-501 ◽  
Author(s):  
CYNTHIA C. NAST ◽  
XIAO-JING ZUO ◽  
JOHN PREHN ◽  
GABRIEL M. DANOVITCH ◽  
ALAN WILKINSON ◽  
...  
Keyword(s):  
Gene Expression ◽  
Renal Allograft ◽  
Gamma Interferon ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Interferon Gene

Characteristics of a recent isolate of Sarcocystis neurona (SN7) from a horse and loss of pathogenicity of isolates SN6 and SN7 by passages in cell culture

2001 ◽  
Vol 95 (2-4) ◽  
pp. 155-166 ◽  
Author(s):  
J.P. Dubey ◽  
D.E. Mattson ◽  
C.A. Speer ◽  
A.N. Hamir ◽  
D.S. Lindsay ◽  
...  
Keyword(s):  
Cell Culture ◽  
Sarcocystis Neurona

scholarly journals Role of Gamma Interferon in Helicobacter pylori Induction of Inflammatory Mediators during Murine Infection

2002 ◽  
Vol 70 (6) ◽  
pp. 3295-3299 ◽  
Author(s):  
Marygorret Obonyo ◽  
Donald G. Guiney ◽  
Julia Harwood ◽  
Joshua Fierer ◽  
Sheri P. Cole
Keyword(s):  
Helicobacter Pylori ◽  
Gene Knockout ◽  
Gamma Interferon ◽  
Epithelial Cell Line ◽  
Inos Mrna ◽  
Inflammatory Protein ◽  
Ifn Γ ◽  
H Pylori ◽  
Oxide Synthase

ABSTRACT Gamma interferon (IFN-γ) has been proposed to play an important role in Helicobacter-related gastritis. Using the IFN-γ gene knockout (IFN-γ−/−) mouse model and a murine gastric epithelial cell line, GSM06, we demonstrated that Helicobacter pylori maximally induced macrophage inflammatory protein-2 (MIP-2) and inducible nitric oxide synthase (iNOS) mRNA only in wild-type mice. MIP-2 and iNOS mRNA were also induced by H. pylori in GSM06 cells. Induction of cyclooxygenase 2 mRNA through IFN-γ was demonstrated in GSM06 cells. These data indicate that IFN-γ mediates the induction of MIP-2 and iNOS mRNA expression by H. pylori in mice.

scholarly journals Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts.

1986 ◽  
Vol 6 (6) ◽  
pp. 2253-2256 ◽  
Author(s):  
H A Young ◽  
L Varesio ◽  
P Hwu
Keyword(s):  
Gene Expression ◽  
Genomic Dna ◽  
Gamma Interferon ◽  
Biologically Active ◽  
Posttranscriptional Control ◽  
Transfected Cells ◽  
3T3 Fibroblasts ◽  
Nih 3T3 ◽  
Interferon Gene ◽  
Calcium Phosphate Precipitation

Human gamma interferon genomic DNA was introduced into NIH 3T3 fibroblasts by calcium phosphate precipitation and was not expressed in these cells at the cytoplasmic mRNA or protein level. Treatment of the transfected cells with cycloheximide (1 microgram/ml) induced the accumulation of cytoplasmic gamma interferon mRNA and biologically active human gamma interferon. Analysis of the nuclear enriched RNA from untreated cells indicated that human gamma interferon mRNA was present, suggesting that cycloheximide may act by inhibiting a specific nuclease or may enhance the processing or transport of the RNA from the nucleus to the cytoplasm.

Sign in / Sign up

Export Citation Format

Share Document