First full-length genomic sequence of a hepatitis A virus isolated in Argentina shows recombination between subgenotypes IA and IB

Hepatitis A virus is known to cause acute hepatitis and has significant implications for public health throughout the world. In the Republic of Korea, the number of patients with hepatitis A virus infection has been increasing rapidly since 2006. In this study, the Kor-HAV-F strain was identified as subgenotype IIIA by RT-PCR, and its identity was confirmed by nucleotide sequencing and alignment analysis. Moreover, detailed phylogenetic analysis indicated that the Kor-HAV-F strain clustered into subgenotype IIIA, including strains isolated in Japan, Norway, and India. The entire amino acid sequence of the VP1 and 2A regions was compared with that of the reference strains isolated in various countries. We found 2 amino acid changes (T168A and L96P, resp.) in the VP1 and 2A regions, which had not been found in any other hepatitis A virus strain. To our knowledge, this study is the first to report the full-length sequence of a hepatitis A virus isolated in the Republic of Korea.

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Analysis of full-length hepatitis A virus genome in sera from patients with fulminant and self-limited acute type A hepatitis

Journal of Hepatology ◽

10.1016/s0168-8278(01)00074-5 ◽

2001 ◽

Vol 35 (1) ◽

pp. 112-119 ◽

Cited By ~ 66

Author(s):

Keiichi Fujiwara ◽

Osamu Yokosuka ◽

Kenichi Fukai ◽

Fumio Imazeki ◽

Hiromitsu Saisho ◽

...

Keyword(s):

Hepatitis A ◽

Hepatitis A Virus ◽

Type A ◽

Virus Genome ◽

Full Length ◽

Acute Type

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Complete genomic sequence of rapidly replicating strain MB-7 of hepatitis a virus and its characterization in comparison with nucleotide sequence of other hepatitis a virus strain

Molecular Genetics Microbiology and Virology ◽

10.3103/s0891416810010088 ◽

2010 ◽

Vol 25 (1) ◽

pp. 39-46 ◽

Cited By ~ 2

Author(s):

T. Yu. Bondarenko ◽

V. A. Ternovoi ◽

V. A. Svyatchenko ◽

N. N. Kiselev ◽

E. V. Chausov ◽

...

Keyword(s):

Nucleotide Sequence ◽

Virus Strain ◽

Hepatitis A ◽

Genomic Sequence ◽

Hepatitis A Virus ◽

Complete Genomic Sequence ◽

Complete Genomic

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Genomic Sequence of the WHO International Standard for Hepatitis A Virus RNA

Genome Announcements ◽

10.1128/genomea.00402-18 ◽

2018 ◽

Vol 6 (19) ◽

Author(s):

Adrian Jenkins ◽

Rehan Minhas ◽

Clare Morris ◽

Neil Berry

Keyword(s):

Hepatitis A ◽

Genomic Sequence ◽

Hepatitis A Virus ◽

World Health ◽

Design Development ◽

Noncoding Regions ◽

International Standard ◽

Virus Rna ◽

Health Organization ◽

Nucleic Acid Assays

ABSTRACT The World Health Organization (WHO) international standard for hepatitis A virus (HAV) RNA nucleic acid assays was characterized by complete genome sequencing. The entire coding sequence and noncoding regions were assigned HAV genotype IB. This information will aid the design, development, and evaluation of HAV RNA amplification assays.

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Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate)

Journal of General Virology ◽

10.1099/vir.0.80304-0 ◽

2004 ◽

Vol 85 (10) ◽

pp. 2943-2952 ◽

Cited By ~ 86

Author(s):

Ling Lu ◽

Karen Z. Ching ◽

Vanessa Salete de Paula ◽

Tatsunori Nakano ◽

Gunter Siegl ◽

...

Keyword(s):

Hepatitis A ◽

Genomic Sequence ◽

Hepatitis A Virus ◽

Phylogenetic Analyses ◽

Pairwise Comparison ◽

Complete Genomic Sequence ◽

Single Genotype ◽

Close Relationship ◽

Genomic Regions ◽

Complete Genomic

The complete genomic sequence of hepatitis A virus (HAV) CF53/Berne strain was determined. Pairwise comparison with other complete HAV genomic sequences demonstrated that the CF53/Berne isolate is most closely related to the single genotype VII strain, SLF88. This close relationship was confirmed by phylogenetic analyses of different genomic regions, and was most pronounced within the capsid region. These data indicated that CF53/Berne and SLF88 isolates are related more closely to each other than are subtypes IA and IB. A histogram of the genetic differences between HAV strains revealed four separate peaks. The distance values for CF53/Berne and SLF88 isolates fell within the peak that contained strains of the same subtype, showing that they should be subtypes within a single genotype. The complete genomic data indicated that genotypes II and VII should be considered a single genotype, based upon the complete VP1 sequence, and it is proposed that the CF53/Berne isolate be classified as genotype IIA and strain SLF88 as genotype IIB. The CF53/Berne isolate is cell-adapted, and therefore its sequence was compared to that of two other strains adapted to cell culture, HM-175/7 grown in MK-5 and GBM grown in FRhK-4 cells. Mutations found at nucleotides 3889, 4087 and 4222 that were associated with HAV attenuation and cell adaptation in HM175/7 and GMB strains were not present in the CF53/Berne strain. Deletions found in the 5′UTR and P3A regions of the CF53/Berne isolate that are common to cell-adapted HAV isolates were identified, however.

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