Experimental infection of the honeybee (Apis mellifera L.) with the chronic bee paralysis virus (CBPV): infectivity of naked CBPV RNAs

2012 ◽  
Vol 167 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Aurore Chevin ◽  
Frank Schurr ◽  
Philippe Blanchard ◽  
Richard Thiéry ◽  
Magali Ribière
Keyword(s):  
Apis Mellifera ◽  
Experimental Infection ◽  
Paralysis Virus

scholarly journals A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kerstin Seitz ◽  
Katharina Buczolich ◽  
Alžbeta Dikunová ◽  
Pavel Plevka ◽  
Karen Power ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Clinical Symptoms ◽  
Well Being ◽  
Reverse Genetic System ◽  
Progeny Virus ◽  
Molecular Clone ◽  
Depth Analysis ◽  
Paralysis Virus

Abstract Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch’s postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease.

scholarly journals Molecular Identification of Chronic Bee Paralysis Virus Infection in Apis mellifera Colonies in Japan

Viruses ◽  
2012 ◽  
Vol 4 (7) ◽  
pp. 1093-1103 ◽  
Author(s):  
Tomomi Morimoto ◽  
Yuriko Kojima ◽  
Mikio Yoshiyama ◽  
Kiyoshi Kimura ◽  
Bu Yang ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Virus Infection ◽  
Molecular Identification ◽  
Paralysis Virus

scholarly journals Varroa destructor is an effective vector of Israeli acute paralysis virus in the honeybee, Apis mellifera

2010 ◽  
Vol 92 (1) ◽  
pp. 151-155 ◽  
Author(s):  
G. Di Prisco ◽  
F. Pennacchio ◽  
E. Caprio ◽  
H. F. Boncristiani ◽  
J. D. Evans ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Varroa Destructor ◽  
Israeli Acute Paralysis Virus ◽  
Paralysis Virus

scholarly journals Spread of Infectious Chronic Bee Paralysis Virus by Honeybee (Apis mellifera L.) Feces

2007 ◽  
Vol 73 (23) ◽  
pp. 7711-7716 ◽  
Author(s):  
M. Ribière ◽  
P. Lallemand ◽  
A.-L. Iscache ◽  
F. Schurr ◽  
O. Celle ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Disease Dynamics ◽  
Reverse Transcription Pcr ◽  
Route Of Infection ◽  
Experimental Inoculation ◽  
Bee Disease ◽  
Intrathoracic Inoculation ◽  
Indirect Route ◽  
Overt Disease ◽  
Paralysis Virus

ABSTRACT Knowledge of the spreading mechanism of honeybee pathogens within the hive is crucial to our understanding of bee disease dynamics. The aim of this study was to assess the presence of infectious chronic bee paralysis virus (CBPV) in bee excreta and evaluate its possible role as an indirect route of infection. Samples of paralyzed bees were (i) produced by experimental inoculation with purified virus and (ii) collected from hives exhibiting chronic paralysis. CBPV in bee heads or feces (crude or absorbed onto paper) was detected by reverse transcription-PCR. CBPV infectivity was assessed by intrathoracic inoculation of bees with virus extracted from feces and by placement of naive bees in cages previously occupied by contaminated individuals. CBPV RNA was systematically detected in the feces of naturally and experimentally infected bees and on the paper sheets that had been used to cover the floors of units containing bees artificially infected with CBPV or the floor of one naturally infected colony. Both intrathoracic inoculation of bees with virus extracted from feces and placement of bees in contaminated cages provoked overt disease in naive bees, thereby proving that the excreted virus was infectious and that this indirect route of infection could lead to overt chronic paralysis. This is the first experimental confirmation that infectious CBPV particles excreted in the feces of infected bees can infect naive bees and provoke overt disease by mere confinement of naive bees in a soiled environment.

scholarly journals OCCURRENCE OF ACUTE PARALYSIS VIRUS OF THE HONEY BEE (APIS MELLIFERA) IN A HUNGARIAN APIARY INFESTED WITH THE PARASITIC MITE VARROA JACOBSONI

1999 ◽  
Vol 47 (3) ◽  
pp. 319-324 ◽  
Author(s):  
L. Békési ◽  
Brenda V. Ball ◽  
M. Dobos-Kovács ◽  
T. Bakonyi ◽  
M. Rusvai
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Clinical Symptoms ◽  
Varroa Jacobsoni ◽  
Infected Adult ◽  
Long Time ◽  
Parasitic Mite ◽  
Ultrathin Sections ◽  
The Relationship ◽  
Paralysis Virus

Viruses of the honey bee have been known for a long time; however, recently the attention of scientists and apiculturalists has turned towards the relationship between these viruses and the parasitic miteVarroa jacobsoni. Although clinical symptoms indicated the presence of some of the viruses of bees in Hungary, none have previously been isolated or identified. During July unusual adult bee and brood mortality was observed in some colonies of an apiary in Budapest known to be infested withVarroa jacobsoni. Large amounts of acute paralysis virus (APV) were detected serologically in healthy honey bee pupae killed by the injection of a bacteria-free extract of diseased adult bees. Crystalline arrays of 30 nm particles were seen in ultrathin sections of the tissues of injected pupae and naturally infected adult bees. In spite of the application of acaricide treatments the bee population in several colonies had collapsed by the end of summer and the apiary suffered severe wintering losses.

scholarly journals A scientific note on in vitro experimental infection of American foulbrood in honeybees Apis mellifera L

Apidologie ◽  
1999 ◽  
Vol 30 (1) ◽  
pp. 75-75
Author(s):  
Tomoko Sakogawa ◽  
Shigeaki Kataoka ◽  
Atsuko Okayama ◽  
Chie Nakajima ◽  
Toyoaki Hayama
Keyword(s):  
Apis Mellifera ◽  
Experimental Infection ◽  
American Foulbrood ◽  
Scientific Note

Response of the honey bee (Apis mellifera) proteome to Israeli acute paralysis virus (IAPV) infection

2015 ◽  
Vol 93 (9) ◽  
pp. 711-720 ◽  
Author(s):  
Sarah Michaud ◽  
Humberto F. Boncristiani ◽  
Joost W. Gouw ◽  
Micheline K. Strand ◽  
Jeffrey Pettis ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Protein Expression ◽  
Honey Bee ◽  
Host Protein ◽  
Colony Losses ◽  
Israeli Acute Paralysis Virus ◽  
Significant Research ◽  
Ubiquitin Proteasome ◽  
The Impact ◽  
Paralysis Virus

Recent declines in honey bee (Apis mellifera L., 1758) populations worldwide have spurred significant research into the impact of pathogens on colony health. The role of the Israeli acute paralysis virus (IAPV) on hive mortality has become of particular concern since being correlated with colony losses. However, the molecular interactions between IAPV and its host remain largely unknown. To investigate changes in host protein expression during IAPV infection, mass-spectrometry-based quantitative proteomics was used to compare IAPV-infected and healthy pupae. Proteins whose expression levels changed significantly during infection were identified and functional analysis was performed to determine host systems and pathways perturbed by IAPV infection. Among the A. mellifera proteins most affected by IAPV, those involving translation and the ubiquitin–proteasome pathway were most highly enriched and future investigation of these pathways will be useful in identifying host proteins required for infection. This analysis represents an important first step towards understanding the honey bee host response to IAPV infection through the systems-level analysis of protein expression.

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