The association of ribosomal protein L18 (RPL18) with infectious bursal disease virus viral protein VP3 enhances viral replication

2018 ◽  
Vol 245 ◽  
pp. 69-79 ◽  
Author(s):  
Bin Wang ◽  
Xueyan Duan ◽  
Mengjiao Fu ◽  
Yanan Liu ◽  
Yongqiang Wang ◽  
...  
Keyword(s):  
Viral Replication ◽  
Ribosomal Protein ◽  
Disease Virus ◽  
Viral Protein ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Nuclear Factor NF45 Interacts with Viral Proteins of Infectious Bursal Disease Virus and Inhibits Viral Replication

2010 ◽  
Vol 84 (20) ◽  
pp. 10592-10605 ◽  
Author(s):  
Ruth L. O. Stricker ◽  
Sven-Erik Behrens ◽  
Egbert Mundt
Keyword(s):  
Viral Replication ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Molecular Mechanisms ◽  
Viral Proteins ◽  
Infectious Bursal Disease ◽  
Nuclear Factor ◽  
Replication Process ◽  
Bursal Disease ◽  
Infected Cells

ABSTRACT Two of the central issues in developing new strategies to interfere with viral infections concern the identification of cellular proteins involved in viral replication and/or antiviral measures and the dissection of the underlying molecular mechanisms. To gain initial insight into the role of host proteins in the life cycle of infectious bursal disease virus (IBDV), a double-stranded RNA virus, we examined the cellular nuclear factor 45 (NF45). NF45 was previously indicated to be involved in the replication process of other types of RNA viruses. Interestingly, by performing immunofluorescence studies, we found that in IBDV-infected cells the mainly nuclear NF45 accumulated at the sites of viral replication in the cytoplasm. NF45 was shown to specifically colocalize with the viral RNA-dependent RNA polymerase VP1, the capsid protein VP2, and the ribonucleoprotein VP3. Immunoprecipitation experiments indicated protein-protein associations between NF45 and VP1, VP2, and VP3. Expression of the individual VP3 or the combination of expression of VP1 and VP3 did not result in a cytoplasmic accumulation of NF45, which, among other data, showed that recruitment of the cellular protein in infected cells functionally correlates with the viral replication process. Since small interfering RNA(siRNA)-mediated downregulation of NF45 resulted in an approximately 5-fold increase of virus yield, our study suggests that NF45, by association with viral proteins, is part of a yet-uncharacterized cellular defense mechanism against IBDV infections.

scholarly journals Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 247
Author(s):  
Mahmoud Mostafa Azzam ◽  
Shou-qun JIANG ◽  
Jia-li CHEN ◽  
Xia-jing LIN ◽  
Zhong-yong GOU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Disease Virus ◽  
Viral Protein ◽  
Infectious Bursal Disease Virus ◽  
Broiler Chickens ◽  
Infected Group ◽  
Infectious Bursal Disease ◽  
Infected Control ◽  
Bursal Disease

A total of 200 one-day-old male broilers were assigned to five groups, and each group consisted of four replicates with 10 birds per replicate. Chicks were fed the basal diet with 0 (non-infected control), 0 (infected control), 10, 20, and 40 mg/kg soybean isoflavones (SI) for 42 days. At 21 days of age, chickens were inoculated with an infectious bursal dose (causing 50% morbidity) of the infectious bursal disease virus (IBDV) BC 6/85 strain by the eye-drop and nasal route (except for the non-infected group). Average daily gain (ADG) and average daily feed intake (ADFI) decreased (p < 0.05) in broilers infected with infectious bursal disease virus (IBDV) from 22 to 42 days. However, infected broilers fed 10 and 20 mg SI/kg had the maximum (p <0.05) ADG and ADFI from 1 to 42 days. Body weight (BW) increased (p < 0.05) in infected broilers fed the 10 and 20 mg SI /kg diet. The bursa weight at 7 days post-infection (dpi) was increased (p < 0.05) by the supplemental 10 mg SI/kg diet. Infected broilers showed the highest (p < 0.05) bursa lesions, with an average score of 4.0 ± 0.0, while the severity of bursa lesions was decreased (p < 0.05) at 3 dpi and 7 dpi by the supplemental 20 mg SI/kg diet. Supplemental SI at 20 mg/kg decreased (p < 0.05) the viral protein 5 (VP5) mRNA expression at 3 dpi and 7 dpi. The level of interferon gamma (IFNγ) was elevated (p < 0.05) in the infected group at 3 dpi and 7 dpi as compared with the control group, while its level was decreased (p < 0.05) by supplemental 10 mg/kg SI at 3 dpi. The level of nuclear factor κB in the bursal tissue showed the lowest value (p < 0.05) with supplemental 10 and 20 mg SI/kg diet at 7 dpi. Supplemental 10, 20, 40 mg/kg SI improved (p < 0.05) the serum total antioxidant activity (T-AOC) in infected broilers at 3 dpi. In addition, the serum level of malondialdehyde (MDA) decreased (p < 0.05) in the group fed 20 mg/kg SI at 7 dpi. In conclusion, supplemental 10~20 mg/kg SI may have a positive effect on broiler chickens infected with IBDV, probably because SI decrease the severity of bursa lesions and viral protein 5 mRNA expression, and have strong antioxidant activity.

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