Characterization of the nuclear localization sequence of beak and feather disease virus capsid proteins and their assembly into virus-like particles

2020 ◽  
Vol 289 ◽  
pp. 198144 ◽  
Author(s):  
Jui-Kai Chen ◽  
Chiaolong Hsiao ◽  
An-Ru Lo ◽  
Chi-Young Wang
Keyword(s):  
Disease Virus ◽  
Nuclear Localization ◽  
Capsid Proteins ◽  
Nuclear Localization Sequence ◽  
Virus Capsid ◽  
Virus Like Particles ◽  
Localization Sequence ◽  
Feather Disease Virus

scholarly journals Identification and functional characterization of a novel bipartite nuclear localization sequence in ARID1A

2016 ◽  
Vol 469 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Nicholas W. Bateman ◽  
Yutaka Shoji ◽  
Kelly A. Conrads ◽  
Kevin D. Stroop ◽  
Chad A. Hamilton ◽  
...  
Keyword(s):  
Nuclear Localization ◽  
Functional Characterization ◽  
Nuclear Localization Sequence ◽  
Localization Sequence

scholarly journals Characterization of Ku702–NLS as Bipartite Nuclear Localization Sequence for Non-Viral Gene Delivery

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e24615 ◽  
Author(s):  
Johannes Matschke ◽  
Alexander Bohla ◽  
Christof Maucksch ◽  
Rashmi Mittal ◽  
Carsten Rudolph ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nuclear Localization ◽  
Nuclear Localization Sequence ◽  
Viral Gene ◽  
Localization Sequence ◽  
Viral Gene Delivery

scholarly journals Calcineurin-dependent nuclear import of the transcription factor Crz1p requires Nmd5p

2001 ◽  
Vol 154 (5) ◽  
pp. 951-960 ◽  
Author(s):  
Renée S. Polizotto ◽  
Martha S. Cyert
Keyword(s):  
Transcription Factor ◽  
Nuclear Localization ◽  
Nuclear Import ◽  
Protein Phosphatase ◽  
Nuclear Localization Sequence ◽  
Promoter Element ◽  
Hydrophobic Residues ◽  
Localization Sequence ◽  
Identification And Characterization

Calcineurin is a conserved Ca2+/calmodulin-specific serine-threonine protein phosphatase that mediates many Ca2+-dependent signaling events. In yeast, calcineurin dephosphorylates Crz1p, a transcription factor that binds to the calcineurin-dependent response element, a 24-bp promoter element. Calcineurin-dependent dephosphorylation of Crz1p alters Crz1p nuclear localization. This study examines the mechanism by which calcineurin regulates the nuclear localization of Crz1p in more detail. We describe the identification and characterization of a novel nuclear localization sequence (NLS) in Crz1p, which requires both basic and hydrophobic residues for activity, and show that the karyopherin Nmd5p is required for Crz1p nuclear import. We also demonstrate that the binding of Crz1p to Nmd5p is dependent upon its phosphorylation state, indicating that nuclear import of Crz1p is regulated by calcineurin. Finally, we demonstrate that residues in both the NH2- and COOH-terminal portions of Crz1p are required for regulated Crz1p binding to Nmd5p, supporting a model of NLS masking for regulating Crz1p nuclear import.

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