Sitagliptin, a dipeptidyl peptidase-4 inhibitor, attenuates apoptosis of vascular smooth muscle cells and reduces atherosclerosis in diabetic apolipoprotein E–deficient mice

2021 ◽  
pp. 106854
Author(s):  
Bo Li ◽  
Yan Rong Luo ◽  
Qian Zhang ◽  
Shi Hui Fu ◽  
Yun Dai Chen ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Apolipoprotein E ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase 4 ◽  
Dipeptidyl Peptidase 4 Inhibitor ◽  
Deficient Mice

scholarly journals Angiotensin AT1BReceptor Mediates Calcium Signaling in Vascular Smooth Muscle Cells of AT1AReceptor–Deficient Mice

Hypertension ◽  
1998 ◽  
Vol 31 (5) ◽  
pp. 1171-1177 ◽  
Author(s):  
Zhiming Zhu ◽  
Sunny H. Zhang ◽  
Charlotte Wagner ◽  
Armin Kurtz ◽  
Nobuyo Maeda ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Calcium Signaling ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Deficient Mice

scholarly journals Fluorescence demonstration of dipeptidyl peptidase I (cathepsin C) in skeletal, cardiac, and vascular smooth muscles.

1982 ◽  
Vol 30 (2) ◽  
pp. 162-164 ◽  
Author(s):  
W T Stauber ◽  
S H Ong
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Extensor Digitorum Longus ◽  
Smooth Muscles ◽  
Muscle Cells ◽  
Dipeptidyl Peptidase ◽  
Extensor Digitorum ◽  
Cathepsin C

Dipeptidyl peptidase I (cathepsin C) was demonstrated histochemically in soleus, extensor digitorum longus, and cardiac muscles but not in vascular smooth muscle cells of the caudal artery of the rat. The enzyme using Pro-Arg-4-methoxy-beta-naphthylamide as the substrate was found in discrete granules in the striated muscles. The activity was greatest in the soleus muscle, with less activity observed in cardiac tissue, and only a few reactive sites observed in the extensor digitorum longus muscle. Under identical conditions no activity was observed associated with vascular smooth muscle cells. Dipeptidyl peptidase I activity was inhibited completely by 1mM HgCl2 in the incubation solutions and not preserved following conventional chemical fixation techniques.

scholarly journals Expression of apolipoprotein E by cultured vascular smooth muscle cells is controlled by growth state.

1988 ◽  
Vol 107 (3) ◽  
pp. 1207-1213 ◽  
Author(s):  
R A Majack ◽  
C K Castle ◽  
L V Goodman ◽  
K H Weisgraber ◽  
R W Mahley ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Apolipoprotein E ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Northern Analysis ◽  
Aortic Tissue ◽  
Growth State ◽  
Apo E

Rat vascular smooth muscle cells (SMC) in culture synthesize and secrete a approximately 38,000-Mr protein doublet or triplet that, as previously described (Majack and Bornstein. 1984. J. Cell Biol. 99:1688-1695), rapidly and reversibly accumulates in the SMC culture medium upon addition of heparin. In the present study, we show that this approximately 38,000-Mr heparin-regulated protein is electrophoretically and immunologically identical to apolipoprotein E (apo-E), a major plasma apolipoprotein involved in cholesterol transport. In addition, we show that expression of apo-E by cultured SMC varies according to growth state: while proliferating SMC produced little apo-E and expressed low levels of apo-E mRNA, quiescent SMC produced significantly more apo-E (relative to other proteins) and expressed markedly increased levels of apo-E mRNA. Northern analysis of RNA extracted from aortic tissue revealed that fully differentiated, quiescent SMC contain significant quantities of apo-E mRNA. These data establish aortic SMC as a vascular source for apo-E and suggest new functional roles for this apolipoprotein, possibly unrelated to traditional concepts of lipid metabolism.

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