scholarly journals Regulation of the corticosteroid signalling system in rainbow trout HPI axis during confinement stress

2018 ◽  
Vol 258 ◽  
pp. 184-193 ◽  
Author(s):  
Pia Kiilerich ◽  
Arianna Servili ◽  
Sandrine Péron ◽  
Claudiane Valotaire ◽  
Lionel Goardon ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Signalling System ◽  
Hpi Axis ◽  
Confinement Stress

CRF-related peptides contribute to stress response and regulation of appetite in hypoxic rainbow trout

2005 ◽  
Vol 289 (4) ◽  
pp. R982-R990 ◽  
Author(s):  
Nicholas J. Bernier ◽  
Paul M. Craig
Keyword(s):  
Rainbow Trout ◽  
Food Intake ◽  
Plasma Cortisol ◽  
Preoptic Area ◽  
Physiological Role ◽  
Corticotropin Releasing Factor ◽  
Stress Axis ◽  
Mrna Levels ◽  
Hypoxic Conditions ◽  
Hpi Axis

Hypoxia stress suppresses appetite in a variety of fish species, but the mechanisms mediating this response are not known. Therefore, given their anorexigenic and hypophysiotropic properties, we investigated the contribution of forebrain corticotropin-releasing factor (CRF) and urotensin I (UI) to the regulation of food intake and the hypothalamic-pituitary-interrenal (HPI) stress axis in hypoxic rainbow trout. Exposure to 50 and 35% O2 saturation for 24 h decreased food intake by 28 and 48%, respectively. The 35% O2 treatment also increased forebrain CRF and UI mRNA levels, plasma cortisol, and lactate. Exposure for 72 h to the same conditions resulted in similar reductions in food intake, increases in plasma cortisol proportional to the hypoxia severity, and increases in forebrain CRF and UI mRNA levels in the 50% O2 treatment. Relative to saline-infused fish, chronic intracranial infusion of the CRF receptor antagonist α-helical CRF(9–41) reduced the appetite-suppressing effects of 24-h exposure to 35% O2 and blocked the hypoxia-induced increase in plasma cortisol. Finally, forebrain microdissection revealed that 50 and 35% O2 exposure for 24 h specifically increases preoptic area CRF and UI mRNA levels in proportion to the severity of the hypoxic challenge and either has no effect or elicits small decreases in other forebrain regions. These results show that CRF-related peptides play a physiological role in regulating the HPI axis and in mediating at least a portion of the reduction in food intake under hypoxic conditions in rainbow trout and demonstrate that the response of forebrain CRF and UI neurons to this stressor is region specific.

scholarly journals Thermal imprinting modifies adult stress and innate immune responsiveness in the teleost sea bream

2017 ◽  
Vol 233 (3) ◽  
pp. 381-394 ◽  
Author(s):  
Ana Patrícia Mateus ◽  
Rita A Costa ◽  
João C R Cardoso ◽  
Karl B Andree ◽  
Alicia Estévez ◽  
...  
Keyword(s):  
Immune Function ◽  
Acute Stress ◽  
Head Kidney ◽  
Innate Immune ◽  
Sea Bream ◽  
Dopachrome Tautomerase ◽  
Hpi Axis ◽  
Confinement Stress ◽  
The Impact ◽  
Eggs And Larvae

The impact of thermal imprinting on the plasticity of the hypothalamic–pituitary–interrenal (HPI) axis and stress response in an adult ectotherm, the gilthead sea bream (Sparus aurata, L.), during its development was assessed. Fish were reared under 4 thermal regimes, and the resulting adults exposed to acute confinement stress and plasma cortisol levels and genes of the HPI axis were monitored. Changes in immune function, a common result of stress, were also evaluated using histomorphometric measurements of melanomacrophages centers (MMCs) in the head kidney and by monitoring macrophage-related transcripts. Thermal history significantly modified the HPI responsiveness in adult sea bream when eggs and larvae were reared at a higher than optimal temperature (HT, 22°C), and they had a reduced amplitude in their cortisol response and significantly upregulated pituitary pomc and head kidney star transcripts. Additionally, after an acute stress challenge, immune function was modified and the head kidney of adult fish reared during development at high temperatures (HT and LHT, 18–22°C) had a decreased number of MMCs and a significant downregulation of dopachrome tautomerase. Thermal imprinting during development influenced adult sea bream physiology and increased plasma levels of glucose and sodium even in the absence of an acute stress in fish reared under a high–low thermal regime (HLT, 22–18°C). Overall, the results demonstrate that temperature during early development influences the adult HPI axis and immune function in a teleost fish.

scholarly journals RNA-seq Analysis of Early Hepatic Response to Handling and Confinement Stress in Rainbow Trout

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e88492 ◽  
Author(s):  
Sixin Liu ◽  
Guangtu Gao ◽  
Yniv Palti ◽  
Beth M. Cleveland ◽  
Gregory M. Weber ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Rna Seq ◽  
Confinement Stress

Metabolic response in liver and Brockmann bodies of rainbow trout to inhibition of lipolysis; possible involvement of the hypothalamus–pituitary–interrenal (HPI) axis

2015 ◽  
Vol 185 (4) ◽  
pp. 413-423 ◽  
Author(s):  
Marta Librán-Pérez ◽  
Cristina Velasco ◽  
Cristina Otero-Rodiño ◽  
Marcos A. López-Patiño ◽  
Jesús M. Míguez ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Metabolic Response ◽  
Hpi Axis

scholarly journals Duress without stress: Cryptobia infection results in HPI axis dysfunction in rainbow trout

2013 ◽  
Vol 218 (3) ◽  
pp. 287-297 ◽  
Author(s):  
Barry N Madison ◽  
Patrick T K Woo ◽  
Nicholas J Bernier
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Plasma Cortisol ◽  
Head Kidney ◽  
Infected Fish ◽  
Control Fish ◽  
Cortisol Response ◽  
Mrna Levels ◽  
Air Exposure ◽  
Hpi Axis

Despite clear physiological duress, rainbow trout (Oncorhynchus mykiss) infected with the pathogenic haemoflagellateCryptobia salmositicado not appear to mount a cortisol stress response. Therefore, we hypothesized that the infection suppresses the stress response by inhibiting the key effectors of the hypothalamic–pituitary–interrenal (HPI) axis. To test this, we characterized the basal activity of the HPI axis and the cortisol response to air exposure in saline- and parasite-injected fish. All fish were sampled at 4 and 6 weeks post-injection (wpi). While both the treatment groups had resting plasma cortisol levels, the parasite-infected fish had lower levels of plasma ACTH than the control fish. Relative to the control fish, the infected fish had higher mRNA levels of brain pre-optic area corticotrophin-releasing factor (CRF) and pituitary CRF receptor type 1, no change in pituitary POMC-A1, -A2 and -B gene expression, higher and lower head kidney melanocortin 2 receptor mRNA levels at 4 and 6 wpi respectively and reduced gene expression of key proteins regulating interrenal steroidogenesis: StAR, cytochrome P450scc and 11β-hydroxylase. The parasite-infected fish also had a reduced plasma cortisol response to a 60-s air exposure stressor. Superfusion of the head kidney tissues of the parasite-infected fish led to significantly lower ACTH-stimulated cortisol release rates than that observed in the control fish. These novel findings show that infection of rainbow trout withC. salmositicaresults in complex changes in the transcriptional activity of both central and peripheral regulators of the HPI axis and in a reduction in the interrenal capacity to synthesize cortisol.

A multivariate analysis using physiology and behavior to characterize robustness in two isogenic lines of rainbow trout exposed to a confinement stress

2015 ◽  
Vol 140 ◽  
pp. 139-147 ◽  
Author(s):  
Bastien Sadoul ◽  
Isabelle Leguen ◽  
Violaine Colson ◽  
Nicolas C. Friggens ◽  
Patrick Prunet
Keyword(s):  
Multivariate Analysis ◽  
Rainbow Trout ◽  
Isogenic Lines ◽  
Confinement Stress ◽  
And Behavior
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