Considerations for using isolated cell systems to understand cardiac metabolism and biology

2021 ◽  
Vol 153 ◽  
pp. 26-41
Author(s):  
Lindsey A. McNally ◽  
Tariq R. Altamimi ◽  
Kyle Fulghum ◽  
Bradford G. Hill
Keyword(s):  
Cardiac Metabolism ◽  
Cell Systems ◽  
Isolated Cell

scholarly journals Cerebral hemodynamic and metabolic changes in fulminant hepatic failure

2017 ◽  
Vol 75 (7) ◽  
pp. 470-476 ◽  
Author(s):  
Fernando Mendes Paschoal Junior ◽  
Ricardo de Carvalho Nogueira ◽  
Marcelo de Lima Oliveira ◽  
Eric Homero Albuquerque Paschoal ◽  
Manoel Jacobsen Teixeira ◽  
...  
Keyword(s):  
Intracranial Hypertension ◽  
Fulminant Hepatic Failure ◽  
Cerebral Edema ◽  
Hepatic Failure ◽  
Experimental Models ◽  
Metabolic Changes ◽  
Brain Swelling ◽  
Cell Systems ◽  
Cerebral Hemodynamic ◽  
Isolated Cell

ABSTRACT Intracranial hypertension and brain swelling are a major cause of morbidity and mortality of patients suffering from fulminant hepatic failure (FHF). The pathogenesis of these complications has been investigated in man, in experimental models and in isolated cell systems. Currently, the mechanism underlying cerebral edema and intracranial hypertension in the presence of FHF is multi-factorial in etiology and only partially understood. The aim of this paper is to review the pathophysiology of cerebral hemodynamic and metabolism changes in FHF in order to improve understanding of intracranial dynamics complication in FHF.

scholarly journals ddAVP does not stimulate acute changes in levels of medullary trimethylamines in humans.

1993 ◽  
Vol 4 (6) ◽  
pp. 1379-1384
Author(s):  
M J Avison ◽  
S K Van Why ◽  
N J Siegel
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Human Physiology ◽  
Arginine Vasopressin ◽  
Urine Osmolality ◽  
Cell Systems ◽  
Time Period ◽  
Human Volunteers ◽  
1H Nuclear Magnetic Resonance ◽  
Acute Changes ◽  
Isolated Cell

1H nuclear magnetic resonance has been used to determine the effect of acute iv administration of the arginine vasopressin analog 1-(3-mercaptopropionic acid)-8-D-arginine vasopressin monoacetate (ddAVP; 2 micrograms) on renal medullary trimethylamine (TMA) levels in human volunteers. In subjects deprived of food and water for 15 h, urine osmolality (Uosm) was 889 +/- 47 mosmol/kg and had not changed significantly 3 h after ddAVP administration. Medullary TMA did not change significantly over 3 h after ddAVP. In a second group of subjects who were well hydrated, acute ddAVP infusion increased Uosm from 203 +/- 63 to 421 +/- 47 mosmol/kg in 3 h (P < 0.05). However, medullary TMA did not change significantly over this time period. These results indicate that ddAVP, and presumably arginine vasopressin, do not acutely influence medullary TMA levels, and they support the view that results previously reported for animal and isolated cell systems are also applicable to human physiology.

Ultrastructure and Morphology of the Neurospora Crassa Cell Wall Mutants, Slime And Slime X, Using Tem and Sem

1976 ◽  
Vol 34 ◽  
pp. 54-55
Author(s):  
Karen S. Howard ◽  
H. D. Braymer ◽  
M. D. Socolofsky ◽  
S. A. Milligan
Keyword(s):  
Cell Wall ◽  
Neurospora Crassa ◽  
Wild Type ◽  
Morphological Comparison ◽  
Small Areas ◽  
Solid Media ◽  
Thin Sectioning ◽  
X Cells ◽  
Mutant Cells ◽  
Isolated Cell

The recently isolated cell wall mutant slime X of Neurospora crassa was prepared for ultrastructural and morphological comparison with the cell wall mutant slime. The purpose of this article is to discuss the methods of preparation for TEM and SEM observations, as well as to make a preliminary comparison of the two mutants.TEM: Cells of the slime mutant were prepared for thin sectioning by the method of Bigger, et al. Slime X cells were prepared in the same manner with the following two exceptions: the cells were embedded in 3% agar prior to fixation and the buffered solutions contained 5% sucrose throughout the procedure.SEM: Two methods were used to prepare mutant and wild type Neurospora for the SEM. First, single colonies of mutant cells and small areas of wild type hyphae were cut from solid media and fixed with OSO4 vapors similar to the procedure used by Harris, et al. with one alteration. The cell-containing agar blocks were dehydrated by immersion in 2,2-dimethoxypropane (DMP).

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