Differences between coding and non-coding regions in the Trichomonas vaginalis genome: an actin gene as a locus model1Data deposition: The sequence reported in this paper has been deposited in the GeneBank database under the accession no. AF237734.1

We characterized nine human actin genes that we isolated (Engel et al., Proc. Natl. Acad. Sci. U.S.A.78:4674-4678, 1981) from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and α-, β-, and γ-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria we show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5′ and 3′ ends of the coding region of a cloned chicken β-actin cDNA. We conclude, therefore, that the human cytoplasmic actin proteins are encoded by a multigene family.

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Human Cytoplasmic Actin Proteins Are Encoded by a Multigene Family

Molecular and Cellular Biology ◽

10.1128/mcb.2.6.674-684.1982 ◽

1982 ◽

Vol 2 (6) ◽

pp. 674-684

Author(s):

Joanne Engel ◽

Peter Gunning ◽

Larry Kedes

Keyword(s):

Multigene Family ◽

Single Gene ◽

Actin Gene ◽

Coding Region ◽

Coding Regions ◽

Cytoplasmic Actin ◽

Actin Mrna ◽

Dna Measurements ◽

Thermal Stability Of ◽

Restriction Enzyme Maps

We characterized nine human actin genes that we isolated (Engel et al., Proc. Natl. Acad. Sci. U.S.A. 78 :4674-4678, 1981) from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and α-, β-, and γ-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria we show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5′ and 3′ ends of the coding region of a cloned chicken β-actin cDNA. We conclude, therefore, that the human cytoplasmic actin proteins are encoded by a multigene family.

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Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran

Acta Parasitologica ◽

10.2478/s11686-020-00226-x ◽

2020 ◽

Vol 65 (4) ◽

pp. 859-864

Author(s):

Hajar Ziaei Hezarjaribi ◽

Mahbobeh Taghavi ◽

Karan Hasanjani Saravi ◽

Roghiyeh Faridnia ◽

Hamed Kalani ◽

...

Keyword(s):

Molecular Typing ◽

Trichomonas Vaginalis ◽

Clinical Isolates ◽

Actin Gene ◽

The North ◽

North Of Iran

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Prevalence and Genotyping of Trichomonas vaginalis Infected to dsRNA Virus by PCR–Restriction Fragment Length Poly-morphism (RFLP)

Iranian Journal of Parasitology ◽

10.18502/ijpa.v14i2.1137 ◽

2019 ◽

Author(s):

Fariba ORUJZADEH ◽

Fatemeh TABATABAIE ◽

Khadijeh KHANALIHA ◽

Lame AKHLAGHI ◽

Farah BOKHARAEI-SALIM ◽

...

Keyword(s):

Trichomonas Vaginalis ◽

Actin Gene ◽

Medical Sciences ◽

Transmitted Infection ◽

Genotype I ◽

Restriction Fragment Length Poly ◽

Sexually Transmitted ◽

Pcr Rflp ◽

Dsrna Viruses ◽

Genotype E

Background: Trichomonas vaginalis is a prevalent sexually transmitted infection cause trichomoniasis. In this study prevalence and genotype of Iranian isolates of T. vaginalis infected (dsRNA) viruses were evaluated by PCR-RFLP and obtained patterns were then confirmed by sequence analysis and genotype of these Iranian isolates confirmed again. Methods: Ten strains of T.vaginalis were collected from 1700 vaginal samples of women referred to hospitals associated with Iran University of Medical Sciences in Tehran, Iran during Feb 2016 to Jul 2017, evaluated in points of infection to T. vaginalis Virus (TVV-1) were used in a PCR-RFLP. All of ten isolates of T. vaginalis were examined by designed nested PCR for actin gene and then digestion patterns of three endonuclease enzymes of HindII, MseI and RsaI were evaluated and genotype of these isolates was defined. Results: By combination of fragments pattern of three enzymes of HindII, RsaI and MseI, three genotypes were found; six genotypes E, two genotypes G and two genotypes I. The most dominant genotypes were genotype E. Among four TVV infected isolates two genotype E, one genotype G and one genotype I were found, however among six uninfected T. vaginalis isolates to TVV-1, all of three genotypes were also found. Conclusion: Three genotypes E, G and I in T. vaginalis infected with dsRNA isolates were found, however, these three genotypes in T. vaginalis without virus were also observed. Further study is needed to evaluate genotypes of T. vaginalis, which infected virus in more great T. vaginalis population

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