Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran

2020 ◽  
Vol 65 (4) ◽  
pp. 859-864
Author(s):  
Hajar Ziaei Hezarjaribi ◽  
Mahbobeh Taghavi ◽  
Karan Hasanjani Saravi ◽  
Roghiyeh Faridnia ◽  
Hamed Kalani ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Trichomonas Vaginalis ◽  
Clinical Isolates ◽  
Actin Gene ◽  
The North ◽  
North Of Iran

scholarly journals Molecular typing of Trichomonas vaginalis isolates by actin gene sequence analysis and carriage of T. vaginalis viruses

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Simon C. Masha ◽  
Piet Cools ◽  
Tania Crucitti ◽  
Eduard J. Sanders ◽  
Mario Vaneechoutte
Keyword(s):  
Sequence Analysis ◽  
Molecular Typing ◽  
Trichomonas Vaginalis ◽  
Gene Sequence ◽  
Actin Gene ◽  
Gene Sequence Analysis

scholarly journals Molecular typing of Trichomonas vaginalis circulating in Ukraine based on actin gene polymorphism

2019 ◽  
pp. 8-12
Author(s):  
A. P. Belozorov ◽  
◽  
P. V . Fedorich ◽  
G. I . Mavrov ◽  
A. D. Zelenskaya ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Molecular Typing ◽  
Trichomonas Vaginalis ◽  
Actin Gene

scholarly journals Role of Aminoglycoside-Modifying Enzymes (AMEs) in Resistance to Aminoglycosides among Clinical Isolates of Pseudomonas aeruginosa in the North of Iran

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Leila Ahmadian ◽  
Zahra Norouzi Bazgir ◽  
Mohammad Ahanjan ◽  
Reza Valadan ◽  
Hamid Reza Goli
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Resistance Pattern ◽  
Dilution Method ◽  
The North ◽  
Agar Diffusion Test ◽  
North Of Iran ◽  
Encoding Genes

In recent years, the prevalence of resistance to aminoglycosides among clinical isolates of Pseudomonas aeruginosa is increasing. The aim of this study was to investigate the role of aminoglycoside-modifying enzymes (AMEs) in resistance to aminoglycosides in clinical isolates of P. aeruginosa. The clinical isolates were collected from different hospitals. Disk agar diffusion test was used to determine the antimicrobial resistance pattern of the clinical isolates, and the minimum inhibitory concentration of aminoglycosides was detected by microbroth dilution method. The PCR was performed for discovery of aminoglycoside-modifying enzyme-encoding genes. Among 100 screened isolates, 43 (43%) isolates were resistant to at least one tested aminoglycosides. However, 13 (13%) isolates were resistant to all tested aminoglycosides and 37 isolates were detected as multidrug resistant (MDR). The resistance rates of P. aeruginosa isolates against tested antibiotics were as follows: ciprofloxacin (41%), piperacillin-tazobactam (12%), cefepime (32%), piperacillin (26%), and imipenem (31%). However, according to the MIC method, 13%, 32%, 33%, and 37% of the isolates were resistant to amikacin, gentamicin, tobramycin, and netilmicin, respectively. The PCR results showed that AAC(6 ′ )-Ib was the most commonly (26/43, 60.4%) identified AME-encoding gene followed by AAC(6 ′ )-IIa (41.86%), APH(3 ′ )-IIb (34.8%), ANT(3 ″ )-Ia (18.6), ANT(2 ″ )-Ia (13.95%), and APH(3 ″ )-Ib (2.32%). However, APH(3 ′ )-Ib was not found in any of the studied isolates. The high prevalence of AME-encoding genes among aminoglycoside-resistant P. aeruginosa isolates in this area indicated the important role of AMEs in resistance to these antibiotics similar to most studies worldwide. Due to the transmission possibility of these genes between the Gram-negative bacteria, we need to control the prescription of aminoglycosides in hospitals.

scholarly journals In vitro activity of colistin against multidrug-resistant Acinetobacter baumannii isolates harboring blaOXA-23-like and blaOXA-24-like genes: A multicenter based study

2020 ◽  
Vol 67 (3) ◽  
pp. 182-186
Author(s):  
Susan Khanjani ◽  
Hadi Sedigh Ebrahim-Saraie ◽  
Mohammad Shenagari ◽  
Ali Ashraf ◽  
Ali Mojtahedi ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Cross Sectional ◽  
The North ◽  
North Of Iran

AbstractThis study was aimed to evaluate occurrence of antibiotic resistance and the presence of resistance determinants among clinical isolates of Acinetobacter baumannii. This cross-sectional study from January to September 2018 was performed on 59 A. baumannii strains isolated from clinical samples in the north of Iran. Isolates were identified by standard microbiologic tests and molecular method. Antimicrobial susceptibility testing was carried out by disk diffusion and broth microdilution methods. The presence of carbapenem resistance genes was detected by PCR method. All isolates were resistant to cefepime, meropenem, imipenem and ceftazidime. The lowest resistance rate was observed against doxycycline with 33.9%. Minimum inhibitory concentration (MIC) results showed that all carbapenem-resistant A. baumannii (CRAB) isolates were susceptible to colistin with MIC50 and MIC90 values of 1/2 µg/mL. Among 59 CRAB, blaOXA-23-like was the most prevalent gene (86.4%) followed by blaOXA-24-like (69.5%). Meanwhile, none of the clinical isolates harbored blaOXA-58-like gene. We found a high prevalence of CRAB strains harboring OXA-type carbapenemases in the north of Iran. Our results suggests that the presence of OXA-type genes was not directly correlated with the increase of imipenem MIC level, but can be clinically important as they contribute to the selection of CRAB strains.

Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR-RFLP in Iran

2015 ◽  
Vol 159 ◽  
pp. 259-263 ◽  
Author(s):  
Zohreh Momeni ◽  
Javid Sadraei ◽  
Bahram Kazemi ◽  
Abdolhossein Dalimi
Keyword(s):  
Molecular Typing ◽  
Trichomonas Vaginalis ◽  
Actin Gene ◽  
Pcr Rflp

Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

2019 ◽  
Vol 19 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Saba Fakhrieh Asl ◽  
Mehrnaz Pourvahedi ◽  
Ali Mojtahedi ◽  
Mohammad Shenagari
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Diseases ◽  
Specific Primers ◽  
The North ◽  
Different Types ◽  
North Of Iran ◽  
Gastric Biopsies ◽  
Pcr Method ◽  
Non Ulcer Dyspepsia ◽  
H Pylori

Objective:Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.Methods:The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.Results:Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).Conclusion:In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

High Parasitic Contamination of Soil Samples in the North of Iran: a Potential Risk of Parasitic Infection for Tourists

2020 ◽  
Vol 20 ◽  
Author(s):  
Beheshteh Haghparast-kenari ◽  
Tooran Nayeri ◽  
Shahabeddin Sarvi ◽  
Mohammad Taghi Rahimi ◽  
Ehsan Ahmadpour ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Urban Areas ◽  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Animal Husbandry ◽  
Soil Samples ◽  
Human Society ◽  
Public Parks ◽  
The North ◽  
North Of Iran

Background: Soil-transmitted parasites (STPs) are significant intestinal parasites that infect humans and animals and impose considerable burdens on human society and animal husbandry industries. Therefore, the present study aimed to determine the prevalence of parasitic elements of soil samples collected from the north of Iran. Methods: A total of 256 soil samples were collected from public parks, public places, vegetable gardens, sand heaps, and shadow areas near houses in the north of Iran and examined using the sucrose flotation method. Results: Out of 256 examined samples, 131 (51.2%) ones showed parasitic contamination including larvae (43%), oocysts (14.1%), and different eggs (6.6%). According to the results, the most and least common parasites observed in the samples were larvae (43%), as well as Toxascaris leonina, and Trichuris trichiura (0.4%), respectively. Moreover, the most and least contaminated locations were sand heaps (62.5%) and shadow areas near houses (45.6%), respectively. Regarding cities, Behshahr (68.3%) and Sari (67.5%) had the highest contaminated soil samples, whereas Chalus (37.5%) showed the lowest contamination. On the other hand, rural samples showed more contamination, compared to urban areas (P< 0.05). Conclusion: The findings of the present study indicate that the overall prevalence of STPs in examined areas and highly contaminated soil samples can be considered as a potential source of human contamination particularly tourists with STPs.

scholarly journals Chromosome-Encoded Class D β-Lactamase OXA-23 in Proteus mirabilis

2002 ◽  
Vol 46 (6) ◽  
pp. 2004-2006 ◽  
Author(s):  
R. Bonnet ◽  
H. Marchandin ◽  
C. Chanal ◽  
D. Sirot ◽  
R. Labia ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Proteus Mirabilis ◽  
Molecular Typing ◽  
Clinical Isolates ◽  
Clonal Strain ◽  
Class D ◽  
Chromosome Fragments

ABSTRACT Ten nonrepetitive Proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of Clermont-Ferrand, France, produced class D carbapenemase OXA-23. MICs of imipenem were 0.25 to 0.5 μg/ml for these clinical isolates. Molecular typing revealed that the 10 P. mirabilis isolates originated from the same clonal strain. Hybridization of I-CeuI-generated chromosome fragments with a bla OXA-23 probe showed that the gene was chromosome encoded in the P. mirabilis strain.

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