Characterization of immobilized enzymes by flow-injection techniques with variable forward flow

1989 ◽  
Vol 218 ◽  
pp. 291-301 ◽  
Author(s):  
Jaromir Ruzicka ◽  
Josef Flossdorf

Flow injection is now a widely used tool in the analytical laboratory for sample presentation to a range of optical and electrochemical detectors (Ruzicka & Hansen 1988; Valcarcel & Luque de Castro 1987). In addition to this basic but none the less important function, it provides a means to perform on-line physical and chemical treatment of the sample. Physical techniques such as dialysis and solvent extraction can significantly improve the total analysis time by minimizing off-line manual operations (Worsfold 1988). Chemical techniques can improve both selectivity and sensitivity and in this regard incorporation of solid phase reactors is particularly important, e.g. immobilized enzymes and antibodies and oxidation and reduction columns can be used to chemically modify the sample and ion-exchange resins and solid adsorbents can be used to preconcentrate the analyte or remove matrix interferences (Worsfold 1988).


The Analyst ◽  
2021 ◽  
Author(s):  
Harald Schoeny ◽  
Evelyn Rampler ◽  
Yasin El Abiead ◽  
Felina Hildebrand ◽  
Olivia Zach ◽  
...  

We propose a fully automated novel workflow for lipidomics based on flow injection- followed by liquid chromatography high resolution mass spectrometry (FI/LC-HRMS). The workflow combined in-depth characterization of the lipidome...


Author(s):  
P. J. Worsfold ◽  
E. P. Achterberg ◽  
A. R. Bowie ◽  
R. Sandford ◽  
V. Cannizzaro ◽  
...  

1997 ◽  
Vol 244 (1) ◽  
pp. 55-61 ◽  
Author(s):  
M. Becker ◽  
U. Spohn ◽  
R. Ulbrich-Hofmann

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