ω-3 Polyunsaturated fatty acids—modulation of voltage-dependent L-type Ca2+ current in guinea-pig tracheal smooth muscle cells

1998 ◽  
Vol 355 (2-3) ◽  
pp. 257-266 ◽  
Author(s):  
Hisanori Hazama ◽  
Toshiaki Nakajima ◽  
Michiko Asano ◽  
Kuniaki Iwasawa ◽  
Toshihiro Morita ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Smooth Muscle ◽  
Guinea Pig ◽  
Polyunsaturated Fatty Acids ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Tracheal Smooth Muscle ◽  
Voltage Dependent

Voltage-dependent calcium channel current in isolated gallbladder smooth muscle cells of guinea pig

1993 ◽  
Vol 264 (6) ◽  
pp. G1066-G1076 ◽  
Author(s):  
T. Shimada
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Time Course ◽  
Reversal Potential ◽  
High Sensitivity ◽  
Muscle Cells ◽  
Patch Clamp Technique ◽  
Decay Component ◽  
Voltage Dependent

The voltage-dependent Ca2+ current was studied in enzymatically dispersed guinea pig gallbladder smooth muscle cells using the whole cell patch-clamp technique. Depolarizing voltage (V) steps induced an inward current (I) that was carried by Ca2+. The threshold potential was -40 to -30 mV, the maximal current was observed at +10 to +20 mV, and the reversal potential was around +80 mV. I-V curves obtained with holding potentials of -80 and -40 mV were not significantly different. This current had a high sensitivity to dihydropyridine drugs, and the Ba2+ or Sr2+ current was larger than the Ca2+ current. Activation was accelerated by increasing the membrane potential. In general, the time course of decay was well fitted by the sum of two exponentials, but consideration of a third (ultra-slow) decay component was also necessary when the current generated by a 2-s command pulse was analyzed. Superimposition of activation and inactivation curves showed the presence of a significant window current. Carbachol suppressed the Ca2+ current only when the pipette contained a low concentration of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. These results show that the L-type Ca2+ current is dominant in gallbladder smooth muscle cells and may contribute to excitation-contraction coupling.

Cromakalim-induced membrane current in guinea-pig tracheal smooth muscle cells

2000 ◽  
Vol 389 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Yuichiro Matsushita ◽  
Satoshi Henmi ◽  
Katsuhiko Muraki ◽  
Yuji Imaizumi ◽  
Minoru Watanabe
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Membrane Current ◽  
Tracheal Smooth Muscle ◽  
Induced Membrane

scholarly journals Effects of KRN2391 on membrane currents in tracheal smooth muscle cells of the guinea-pig

1997 ◽  
Vol 73 ◽  
pp. 257
Author(s):  
Yuichiro Matsushita ◽  
Katsuhiko Muraki ◽  
Yun Imaizumi ◽  
Minoru Watanabe
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Membrane Currents ◽  
Tracheal Smooth Muscle
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