Ovarian Response to Recombinant Human Follicle-Stimulating Hormone in Luteinizing Hormone-Depleted Women: Examination of the Two Cell, Two Gonadotropin Theory

SUMMARY Eight women with secondary amenorrhoea were treated, four at each of two centres. They were each given four preparations of gonadotrophin having different ratios of follicle-stimulating hormone (FSH) to luteinizing hormone (LH) and the order of treatment with the preparations was determined at each centre by a latin square design. At the completion of these treatments each patient received a fifth preparation of gonadotrophin. The dosage of FSH was determined for each woman by a previous sensitivity test and remained constant for each of her 5 months of treatment. Each gonadotrophin preparation was given in approximately equal daily injections on days 1, 2 and 3 of each course of treatment and was invariably followed by a single injection of 12000 i.u. human chorionic gonadotrophin on the 10th day. Treatment was assessed by the measurement of the excretion of oestriol and pregnanediol, by vaginal cytology, by the degree of ferning of cervical mucus and by the basal body temperature (BBT). The administration of gonadotrophin with a ratio of FSH:LH < 1·0 produced a greater excretion of oestriol than gonadotrophin with a ratio of FSH:LH > 4·0. There was no significant loss of sensitivity to treatment with time. The karyopyknotic index was correlated with the excretion of oestriol but neither was of use in predicting the other because of the wide scatter. There was no correlation between the degree of ferning and the excretion of oestriol. Assessments of ovulation by the pregnanediol response and by the BBT were in good agreement. These results are discussed.

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RESPONSE OF MOUSE GRAAFIAN FOLLICLES IN ORGAN CULTURE TO VARYING DOSES OF FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0650027 ◽

1975 ◽

Vol 65 (1) ◽

pp. 27-32 ◽

Cited By ~ 15

Author(s):

P. NEAL ◽

T. G. BAKER

Keyword(s):

Luteinizing Hormone ◽

Organ Culture ◽

Oocyte Maturation ◽

Mode Of Action ◽

Follicle Stimulating Hormone ◽

Ovarian Response ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Stimulating Hormone

SUMMARY The response of mouse ovaries maintained in organ culture to follicle-stimulating hormone (FSH), luteinizing hormone (LH) and human chorionic gonadotrophin (HCG) was assessed using quantitative histological and radioimmunoassay techniques. In terms of the induction of preovulatory maturation in follicular oocytes, 1 μg FSH/ml medium was as effective as 10 μg LH/ml. The lowest doses of HCG and LH used (0·2 i.u./ml and 1 μg/ml respectively) had no effect on oocyte maturation, whereas the response to FSH was virtually unchanged irrespective of dose (1–10 μg/ml). When the level of progesterone in the medium at the end of organ culture was used as an index of ovarian response, LH was more effective than FSH and HCG, although all the hormones induced a significant increase, irrespective of dose. These results are discussed in terms of the mode of action of gonadotrophins in the processes culminating in ovulation.

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THE RAT OVARIAN AUGMENTATION METHOD FOR FOLLICLE STIMULATING HORMONE

Acta Endocrinologica ◽

10.1530/acta.0.0700647 ◽

1972 ◽

Vol 70 (4) ◽

pp. 647-653 ◽

Cited By ~ 8

Author(s):

Peter Christiansen

Keyword(s):

Growth Hormone ◽

Luteinizing Hormone ◽

Urine Sample ◽

Positive Response ◽

Follicle Stimulating Hormone ◽

Human Growth ◽

Ovarian Response ◽

Thyroid Stimulating Hormone ◽

Inert Material ◽

Stimulating Hormone

ABSTRACT The specificity of the rat ovarian augmentation method (Steelman & Pohley 1953) for follicle stimulation hormone (FSH) has been studied. The addition of luteinizing hormone (LH), thyroid stimulating hormone (TSH), prolactin, ACTH and human growth hormone (GH) did not influence the ovarian response either to ovine or to human FSH. Urine extracts from 2 infants gave no positive response with a dose of one 24 hour urine sample per rat, indicating that inert material in the urine extracts does not influence the ovarian response.

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