W1975 Genetic Inhibition of Telomerase Leads to Induction of ALT in Immortalized Human Esophageal Epithelial Cells and in Generated Human Squamous Cancer Cells

The role of calcium in modulating cellular replication and differentiation has been described for various cell types. In the present study, the effects of Ca++ on the growth and differentiation of cultured rat esophageal epithelial cells was investigated.Epithelial cells were isolated from esophagi taken from 8 week-old male CDF rats by the enzymatic dissociation method of Kaighn. The cells were cultured in PFMR-4 medium supplemented with 0.25 mg/ml dialyzed fetal bovine serum, 5 ng/ml epidermal growth factor, 10-6 M hydrocortisone 10-6 M phosphoethanolamine, 10-6 M ethanolamine, 5 pg/ml insulin, 5 ng/ml transferrin, 10 ng/ml cholera toxin and 50 ng/ml garamycin at 36.5°C in a humidified atmosphere of 3% CO2 in air. At weekly intervals, the cells were subcultured with a solution containing 1% polyvinylpyrrolidone, 0.01% EGTA, and 0.05% trypsin. After various passages, the replication rate of the cells in PFMR-4 medium containing from 10-6 M to 10-3 M Ca++ was determined using a clonal growth assay.

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Inhibition of telomerase by mutant template telomerase RNA and anti-telomerase short interfering RNA induces ALT in immortalized human esophageal epithelial cells

Zeitschrift für Gastroenterologie ◽

10.1055/s-2006-950885 ◽

2006 ◽

Vol 44 (08) ◽

Cited By ~ 1

Author(s):

M Döbele ◽

A von Werder ◽

C Fulda ◽

S Heeg ◽

G Gössel ◽

...

Keyword(s):

Epithelial Cells ◽

Telomerase Rna ◽

Short Interfering Rna ◽

Esophageal Epithelial Cells ◽

Interfering Rna

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Biomimetic Sulfated GAGs Maintain Differentiation Markers of Breast Epithelial Cells and Preferentially Inhibit Proliferation of Cancer Cells

SSRN Electronic Journal ◽

10.2139/ssrn.3688763 ◽

2020 ◽

Author(s):

Zeina Habli ◽

Nataly Naser Al Deen ◽

Waddah Malaeb ◽

Nadine Mahfouz ◽

Angela Mermerian ◽

...

Keyword(s):

Epithelial Cells ◽

Cancer Cells ◽

Differentiation Markers ◽

Breast Epithelial Cells ◽

Breast Epithelial

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Immune negative regulator TIPE2 inhibits cervical squamous cancer progression through Erk1/2 signaling

Open Life Sciences ◽

10.1515/biol-2019-0059 ◽

2019 ◽

Vol 14 (1) ◽

pp. 528-536

Author(s):

Li-Qiong Huang ◽

Bo Zheng ◽

Yi He

Keyword(s):

Cervical Cancer ◽

Western Blot ◽

Cancer Cells ◽

Signaling Pathway ◽

Erk Signaling ◽

Over Expression ◽

Squamous Cancer ◽

Cancer Tissues ◽

Proliferation And Invasion ◽

Cervical Squamous Cancer

AbstractTumor necrosis factor (TNF)-α-induced protein-8-like 2, or TIPE2, is a newly found immune negative regulatory molecule. This study further investigated the role of TIPE2 on proliferation and invasion of cervical squamous cancer cells. Expression of TIPE2 was compared in cervical squamous cancer tissues and adjacent normal tissues by Western blot and immunohistochemistry (IHC). Cervical squamous cancer cell lines, SiHa and C33A, were transfected with recombinant plasmid encoding TIPE2 and tested for cytologic characteristics. The impact of TIPE2 on phosphorylation of extracellular signal-regulated kinase (Erk) signaling pathway was also tested by Western blot analysis of key factors. TIPE2 expression was higher in cervical cancer tissues than that in normal tissue. IHC score of tumor tissue was negatively associated with lymphatic metastasis. Over expression of TIPE2 effectively inhibited the proliferation of cervical cancer cells. Wound healing and transwell assay showed that over expression of TIPE2 suppressed cell migration and invasion in vitro. Meanwhile, phosphorylation of Erk1/2 and upstream mitogen-activated protein kinase kinase (MEK) 1/2 was reduced by TIPE2. TIPE2 is negatively related with development of cervical squamous cancer. TIPE2 is an inhibitory factor of proliferation and invasion of cervical squamous cancer cells, probably through inhibiting Erk signaling pathway.

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