Su2038 Effects of Ischemia and Reperfusion on Rat Enteric Glial Cells Expressing the P2X2 and P2X7 Receptors

Multiple sclerosis (MS) has been considered to specifically affect the central nervous system (CNS) for a long time. As autonomic dysfunction including dysphagia can occur as accompanying phenomena in patients, the enteric nervous system has been attracting increasing attention over the past years. The aim of this study was to identify glial and myelin markers as potential target structures for autoimmune processes in the esophagus. RT-PCR analysis revealed glial fibrillary acidic protein (GFAP), proteolipid protein (PLP), and myelin basic protein (MBP) expression, but an absence of myelin oligodendrocyte glycoprotein (MOG) in the murine esophagus. Selected immunohistochemistry for GFAP, PLP, and MBP including transgenic mice with cell-type specific expression of PLP and GFAP supported these results by detection of (1) GFAP, PLP, and MBP in Schwann cells in skeletal muscle and esophagus; (2) GFAP, PLP, but no MBP in perisynaptic Schwann cells of skeletal and esophageal motor endplates; (3) GFAP and PLP, but no MBP in glial cells surrounding esophageal myenteric neurons; and (4) PLP, but no GFAP and MBP in enteric glial cells forming a network in the esophagus. Our results pave the way for further investigations regarding the involvement of esophageal glial cells in the pathogenesis of dysphagia in MS.

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Protective role of berberine on ulcerative colitis through modulating enteric glial cells–intestinal epithelial cells–immune cells interactions

Acta Pharmaceutica Sinica B ◽

10.1016/j.apsb.2019.08.006 ◽

2020 ◽

Vol 10 (3) ◽

pp. 447-461 ◽

Cited By ~ 10

Author(s):

Heng Li ◽

Chen Fan ◽

Huimin Lu ◽

Chunlan Feng ◽

Peilan He ◽

...

Keyword(s):

Ulcerative Colitis ◽

Epithelial Cells ◽

Glial Cells ◽

Immune Cells ◽

Intestinal Epithelial Cells ◽

Protective Role ◽

Intestinal Epithelial ◽

Enteric Glial Cells

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The multiple faces of inflammatory enteric glial cells: is Crohn’s disease a gliopathy?

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00016.2018 ◽

2018 ◽

Vol 315 (1) ◽

pp. G1-G11 ◽

Cited By ~ 10

Author(s):

Camille Pochard ◽

Sabrina Coquenlorge ◽

Marie Freyssinet ◽

Philippe Naveilhan ◽

Arnaud Bourreille ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Glial Cells ◽

Physiological Role ◽

Enteric Neurons ◽

Intestinal Epithelial ◽

Intestinal Epithelial Barrier ◽

Enteric Glial Cells ◽

Glial Reactivity

Gone are the days when enteric glial cells (EGC) were considered merely satellites of enteric neurons. Like their brain counterpart astrocytes, EGC express an impressive number of receptors for neurotransmitters and intercellular messengers, thereby contributing to neuroprotection and to the regulation of neuronal activity. EGC also produce different soluble factors that regulate neighboring cells, among which are intestinal epithelial cells. A better understanding of EGC response to an inflammatory environment, often referred to as enteric glial reactivity, could help define the physiological role of EGC and the importance of this reactivity in maintaining gut functions. In chronic inflammatory disorders of the gut such as Crohn’s disease (CD) and ulcerative colitis, EGC exhibit abnormal phenotypes, and their neighboring cells are dysfunctional; however, it remains unclear whether EGC are only passive bystanders or active players in the pathophysiology of both disorders. The aim of the present study is to review the physiological roles and properties of EGC, their response to inflammation, and their role in the regulation of the intestinal epithelial barrier and to discuss the emerging concept of CD as an enteric gliopathy.

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