O599 USE OF COMMERCIAL β-HUMAN CHORIONIC GONADOTROPIN (β-hCG) KIT IN DETECTING VAGINAL FLUID β-hCG IN CASES OF PREMATURE RUPTURE OF MEMBRANES

2012 ◽  
Vol 119 ◽  
pp. S472-S472
Author(s):  
A.C. Santiago
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Vaginal Fluid ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Β Hcg

scholarly journals Qualitative Determination of Human Chorionic Gonadotropin in Vaginal Washings for the Early Diagnosis of Premature Rupture of Fetal Membranes

2017 ◽  
Vol 39 (07) ◽  
pp. 317-321
Author(s):  
Camila Dartibale ◽  
Nelson Uchimura ◽  
Luiz Nery ◽  
Angelita Schumeish ◽  
Liza Uchimura ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Pregnant Women ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Fetal Membranes ◽  
Vaginal Fluid ◽  
Premature Rupture ◽  
Significant Difference ◽  
Β Hcg ◽  
Hcg Test

Purpose This study aimed to evaluate and validate the qualitative human chorionic gonadotropin β subunit (β-hCG) test of the vaginal fluid washings of pregnant women with premature rupture of fetal membranes (PROM). Methods Cross-sectional study of pregnant women between gestational weeks 24 and 39 who underwent consultations in one of our institutions. They were divided into two groups: group A (pregnant women clinically diagnosed with PROM) and group B (pregnant women without loss of amniotic liquid). The patients were subjected to a vaginal fluid washing with 3 mL of saline solution, which was aspirated subsequently with the same syringe. The solution was immediately sent to the laboratory to perform the vaginal β-hCG test with cut-off points of 10 mIU/mL (β-hCG-10) and/or 25 mIU/mL (β-hCG-25). Results The β-hCG-10 test of the vaginal secretion was performed in 128 cases. The chi-squared test with Yates' correction showed a statistically significant difference between the 2 groups (p = 0.0225). The sensibility, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy parameters were 77.1%, 43.6%, 52.3%; 70.4%; and 58.6% respectively. The β-hCG-25 test of the vaginal washing was performed in 49 cases. The analysis by Fisher's exact test showed a statistically significant difference between the groups (p = 0.0175). The sensibility, specificity, PPV, NPV, and accuracy parameters were 44.4%, 87.1%, 66.6%; 72.9%; and 71.4% respectively. Conclusions The β-hCG-25 test showed better accuracy for the diagnosis of PROM, and can corroborate the early diagnosis of PROM because it is a simple and quick exam.

Evaluation of Vaginal Fluid b-Human Chorionic Gonadotropin for Diagnosis of Premature Rupture of Membranes

2018 ◽  
Vol 86 (March) ◽  
pp. 597-603
Author(s):  
AHMED M. ABBAS, M.Sc. SHAHINAZ H. EL-SHORBAGY, M.D. ◽  
AMAL S. EL-BANDARY, M.D. AHMED M. EL-ATTAR, M.D.
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Vaginal Fluid ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture

Practicability of using vaginal fluid markers in detecting premature rupture of membranes

2003 ◽  
Vol 40 (5) ◽  
pp. 542-545 ◽  
Author(s):  
Chen Yi Ni ◽  
Wang Xue Jia ◽  
Wu Min Yi ◽  
Lu Hai Feng ◽  
Lin Zhi Yu
Keyword(s):  
Sensitivity And Specificity ◽  
Roc Analysis ◽  
Random Access ◽  
Diagnostic Sensitivity ◽  
Vaginal Fluid ◽  
Emergency Setting ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Β Hcg ◽  
Diagnostic Sensitivity And Specificity

Background: Premature rupture of membranes (PROM) may cause intra-uterine infection and fetal death. A diagnostic tool that is non-invasive, specific and quick is needed to predict PROM. Human chorionic gonadotrophin (hCG), α-fetoprotein (AFP) and interleukin 6 (IL-6), which are present in vaginal fluid, were reported to be potential markers for PROM but have not yet been used in the clinic. This study was designed in a daily routine semi-emergency setting to evaluate the clinical practicability of using these markers in diagnosing PROM. Methods: Using a random-access automated luminescence immunoassay system, 81 vaginal washing samples collected from third trimester pregnant women (43 from PROM patients, 38 from patients with intact membranes) were analysed for β-hCG, AFP and IL-6 in a semi-emergency setting. The Mann-Whitney U-test was used to test the difference between the two groups. Receiver operator curve (ROC) analysis was used to evaluate the performance of the three markers and to determine the cut-off value for a positive diagnosis. Results: Vaginal fluid concentrations of the three markers were significantly different ( P < 0.001) between the two groups. ROC analysis indicated that AFP had a 97.7% diagnostic sensitivity and 100% specificity; the other two markers had lower diagnostic sensitivity and specificity (95.3% and 89.5% for β-hCG and 83.7% and 78.9% for IL-6, respectively). Discussion: This work demonstrates that, of the three markers investigated, AFP has the highest diagnostic sensitivity and specificity. Using the 'stat' function provided by the automated luminescence immunoassay system, the reporting time of the results was less than 1h. We conclude that vaginal fluid AFP measured by random-access automated luminescence immunoassay is an ideal marker for the diagnosis of PROM. The technique could be introduced into the laboratory as a semi-emergency service to meet clinical needs.

scholarly journals The value of measurement of vaginal fluid creatinine and beta-human chorionic gonadotropin in the diagnosis of premature rupture of membranes

2021 ◽  
Author(s):  
Zafer Bütün
Keyword(s):  
Predictive Value ◽  
Accurate Method ◽  
Control Group ◽  
Lower Sensitivity ◽  
Vaginal Fluid ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Beta Human Chorionic Gonadotropin ◽  
Significant Difference ◽  
Β Hcg

Objective: The purpose of the present study is to evaluate β-human chorionic go- nadotropin (β-hCG) and creatinine levels in the vaginal fluid regarding to diagnosis of premature rupture of membranes (PROM). Material and Methods: This study was conducted on 150 pregnant women in the third trimester (28–40 weeks). The patients were grouped as: (1) PPROM group (75 cases) and (2) intact membranes as control group (75 cases). Three milliliters of ster- ile normal saline were inserted into the posterior fornix of the vagina and then vaginal fluid was aspirated. Creatinine and β-hCG levels in the vaginal fluid were measured β-hCG and creatinine levels were compared between the two groups. Results: The mean vaginal fluid level in Groups 1 and 2 was 0.60±0.72 (0.37) and 0.22±0.11 (0.2) for creatinine and β-hCG which was positive in 411.69±605.65 (146) and 12.71±24.63 (3.9), respectively. There was a statistically significant difference regarding to mean creatinine and β-hCG levels between two groups (p<0.001). Sen- sitivity, specificity, positive predictive value, negative predictive value, and accuracy were all 66.67%, 96%, 94.3%, 74.3%, and 81.3% for creatinine and 94.6%, 82.6%, 84.52%, 93.94%, and 88.67% for β-hCG in detecting PROM with a cutoff value of 0.21 mg/dl for creatinine and 16 mIU/ml for β-hCG. Conclusion: Measuring of β-hCG level in vaginal fluid is accurate, cheap, and simple methods in the diagnosis of PROM. Furthermore, measuring of creatinine level is a simple and accurate method with a lower sensitivity and accuracy than for β-hCG.

scholarly journals Qualitative Human Chorionic Gonadotropin Testing in Vaginal Fluid for Diagnosis of Preterm Premature Rupture of Membrane

1970 ◽  
Vol 4 (2) ◽  
pp. 28-31
Author(s):  
Reeti Mehra ◽  
Anju Huria ◽  
Beena Mahaur
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Vaginal Fluid ◽  
Premature Rupture ◽  
Preterm Premature Rupture

DOI: http://dx.doi.org/10.3126/njog.v4i2.5043 NJOG 2010 Jan-Feb; 4(2): 28-31

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