scholarly journals Localization and function of 5-hydroxytryptamine4 receptor and effect of mosapride in guinea pig stomach

1998 ◽  
Vol 76 ◽  
pp. 295
Author(s):  
Yasuko Sakurai-Yamashita ◽  
Kohei Takada ◽  
Keiko Takemura ◽  
Kimihiro Yamashita ◽  
Kohtaro Taniyama
Keyword(s):  
Guinea Pig ◽  
Guinea Pig Stomach ◽  
And Function

Functional differences of Na+/Ca2+ exchanger expression in Ca2+ transport system of smooth muscle of guinea pig stomach

2005 ◽  
Vol 83 (8-9) ◽  
pp. 791-797 ◽  
Author(s):  
Yasushi Sakai ◽  
Hiroki Kinoshita ◽  
Keiichirou Saitou ◽  
Ikuo Homma ◽  
Koji Nobe ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Guinea Pig ◽  
Gastric Fundus ◽  
Relaxation Cycle ◽  
Gastric Smooth Muscle ◽  
Guinea Pig Stomach ◽  
And Function ◽  
The Relationship ◽  
Ionophore Monensin

The plasma membrane ATP-dependent Ca2+ pump and the Na+/Ca2+ exchanger (NCX) are the major means of Ca2+ extrusion in smooth muscle. However, little is known regarding distribution and function of the NCX in guinea pig gastric smooth muscle. The expression pattern and distribution of NCX isoforms suggest a role as a regulator of Ca2+ transport in cells. Na+ pump inhibition and the consequent to removal of K+ caused gradual contraction in fundus. In contrast, the response was significantly less in antrum. Western blotting analysis revealed that NCX1 and NCX2 are the predominant NCX isoforms expressed in stomach, the former was expressed strongly in antrum, whereas the latter displayed greater expression in fundus. Isolated plasma membrane fractions derived from gastric fundus smooth muscle were also investigated to clarify the relationship between NCX protein expression and function. Na+-dependent Ca2+ uptake increased directly with Ca2+ concentration. Ca2+ uptake in Na+-loaded vesicles was markedly elevated in comparison with K+-loaded vesicles. Additionally, Ca2+ uptake by the Na+- or K+-loaded vesicles was substantially higher in the presence of A23187 than in its absence. The result can be explained based on the assumption that Na+ gradients facilitate downhill movement of Ca2+. Na+-dependent Ca2+ uptake was abolished by the monovalent cationic ionophore, monensin. NaCl enhanced Ca2+ efflux from vesicles, and this efflux was significantly inhibited by gramicidin. Results documented evidence that NCX2 isoform functionally contributes to Ca2+ extrusion and maintenance of contraction-relaxation cycle in gastric fundus smooth muscle.Key words: stomach, smooth muscle, Na+/Ca2+ exchanger (NCX), NCX2.

scholarly journals Regulation of acetylcholine release from the guinea-pig stomach via 5-HT4 receptors.

1994 ◽  
Vol 64 ◽  
pp. 90
Author(s):  
Shogo Matsuyama ◽  
Hideyo Sakjyama ◽  
Koji Nei ◽  
Shigenori Katayama ◽  
Norikazu Sakamoto ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Acetylcholine Release ◽  
Guinea Pig Stomach

Cell loss from human, canine and guinea-pig gastric mucosa measured by DNA radioimmunoassay

1984 ◽  
Vol 66 (6) ◽  
pp. 701-708 ◽  
Author(s):  
B. C. Hurst ◽  
W. D. W. Rees ◽  
A. Garner
Keyword(s):  
Guinea Pig ◽  
Animal Species ◽  
Colorimetric Assay ◽  
Cell Loss ◽  
Mucosal Cell ◽  
Normal Human ◽  
Dna Loss ◽  
Guinea Pig Stomach ◽  
Pavlov Pouch ◽  
Stimulation Of

1. A DNA radioimmunoassay, sensitive in the range 25–1000 ng, has been developed to measure gastric mucosal cell loss. Validity of the assay was based on antibody specificity, absence of interference from gastric contents, parallel tracer displacement by dilutions of gastric and standard DNA, and crossover with colorimetric assay. 2. With this assay, gastric DNA shedding was examined in two animal species and man. In the guinea-pig stomach, DNA loss was 20–100 pg/h and in the canine Pavlov pouch, 260–580pg/h. In the canine Pavlov pouch stimulation of acid secretion by histamine and exposure to exogenous acid increased DNA output. In the normal human stomach DNA loss was 544 k 175 pg/h and in duodenal ulcer patients, 649 k 225 pg/li. 3. DNA radioimmunoassay provides a sensitive and reproducible measure of cell exfoliation from the stomach and may be a useful tool for studying aspects of gastric mucosal defence.

W1363 Real Time Detection and Function of Melatonin Release from the Mucosa of Guinea Pig Ileum

2008 ◽  
Vol 134 (4) ◽  
pp. A-688 ◽  
Author(s):  
Bhavik A. Patel ◽  
Xiaochun Bian ◽  
James Galligan ◽  
Greg M. Swain
Keyword(s):  
Guinea Pig ◽  
Real Time ◽  
Guinea Pig Ileum ◽  
Real Time Detection ◽  
And Function

Actions of cysteinyl leukotrienes in the enteric nervous system of guinea-pig stomach and small intestine

2003 ◽  
Vol 459 (1) ◽  
pp. 27-39 ◽  
Author(s):  
Sumei Liu ◽  
Hong-Zhen Hu ◽  
Chuanyun Gao ◽  
Na Gao ◽  
Guodu Wang ◽  
...  
Keyword(s):  
Nervous System ◽  
Small Intestine ◽  
Guinea Pig ◽  
Enteric Nervous System ◽  
Cysteinyl Leukotrienes ◽  
Guinea Pig Stomach

Capsaicin-induced release of substance P-like immunoreactivity from the guinea-pig stomach

1988 ◽  
Vol 22 (1-2) ◽  
pp. 156
Author(s):  
D. Renzi ◽  
P. Santicioli ◽  
C.A. Maggi ◽  
C. Surrenti ◽  
A. Calabrò ◽  
...  
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
Guinea Pig Stomach

scholarly journals Neuronal release of endogenous dopamine from corpus of guinea pig stomach

1997 ◽  
Vol 273 (5) ◽  
pp. G1044-G1050
Author(s):  
Kazuko Shichijo ◽  
Yasuko Sakurai-Yamashita ◽  
Ichiro Sekine ◽  
Kohtaro Taniyama
Keyword(s):  
Guinea Pig ◽  
Ganglion Cells ◽  
Splanchnic Nerve ◽  
Muscle Layer ◽  
Nerve Fibers ◽  
Endogenous Dopamine ◽  
Dopamine Content ◽  
Release Of Acetylcholine ◽  
Gastric Corpus ◽  
Guinea Pig Stomach

Neuronal release of endogenous dopamine was identified in mucosa-free preparations (muscle layer including intramural plexus) from guinea pig stomach corpus by measuring tissue dopamine content and dopamine release and by immunohistochemical methods using a dopamine antiserum. Dopamine content in mucosa-free preparations of guinea pig gastric corpus was one-tenth of norepinephrine content. Electrical transmural stimulation of mucosa-free preparations of gastric corpus increased the release of endogenous dopamine in a frequency-dependent (3–20 Hz) manner. The stimulated release of dopamine was prevented by either removal of external Ca2+ or treatment with tetrodotoxin. Dopamine-immunopositive nerve fibers surrounding choline acetyltransferase-immunopositive ganglion cells were seen in the myenteric plexus of whole mount preparations of gastric corpus even after bilateral transection of the splanchnic nerve proximal to the junction with the vagal nerve (section of nerves between the celiac ganglion and stomach). Domperidone and sulpiride potentiated the stimulated release of acetylcholine and reversed the dopamine-induced inhibition of acetylcholine release from mucosa-free preparations. These results indicate that dopamine is physiologically released from neurons and from possible dopaminergic nerve terminals and regulates cholinergic neuronal activity in the corpus of guinea pig stomach.

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