The Wilms' tumor gene product, WT1, represses transcription of the platelet-derived growth factor A-chain gene.

The expression of platelet-derived growth factor (PDGF), its receptor, and related genes was studied in the lung tissue of rats exposed to air or 85% O2. PDGF-B chain mRNA was increased by 6 days and PDGF B-type receptor mRNA was increased by 4 and 6 days of exposure to 85% O2. Despite a continued increase of cell division, both PDGF-B chain and B-type receptor mRNAs had returned to control values by 14 days of exposure to 85% O2. PDGF-A chain mRNA was unaffected by exposure to 85% O2. Nuclear runoff transcription analysis confirmed increased transcription of PDGF-B chain mRNA, whereas Western blot analysis of lung homogenates suggested consequent increased translation of PDGF-B chain mRNA to PDGF-BB at 7 days of exposure to 85% O2. Combined immunocytochemistry and autoradiography localized PDGF-BB to the major site of cell division, the pulmonary interstitium. We speculate that the early pulmonary fibroblast hyperplasia observed following exposure to 85% O2 is mediated by increased PDGF-B chain gene expression and may also be mediated by changes in PDGF B-type receptor gene expression.

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Ets-1 Stimulates Platelet-Derived Growth Factor A-Chain Gene Transcription and Vascular Smooth Muscle Cell Growth via Cooperative Interactions With Sp1

Circulation Research ◽

10.1161/01.res.0000141135.36279.67 ◽

2004 ◽

Vol 95 (5) ◽

pp. 479-487 ◽

Cited By ~ 38

Author(s):

Fernando S. Santiago ◽

Levon M. Khachigian

Keyword(s):

Smooth Muscle ◽

Growth Factor ◽

Cell Growth ◽

Smooth Muscle Cell ◽

Gene Transcription ◽

Platelet Derived Growth Factor ◽

Chain Gene ◽

Cooperative Interactions ◽

Smooth Muscle Cell Growth ◽

A Chain

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Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.85.5.1492 ◽

1988 ◽

Vol 85 (5) ◽

pp. 1492-1496 ◽

Cited By ~ 106

Author(s):

D. T. Bonthron ◽

C. C. Morton ◽

S. H. Orkin ◽

T. Collins

Keyword(s):

Growth Factor ◽

Gene Structure ◽

Chromosomal Location ◽

Mrna Splicing ◽

Platelet Derived Growth Factor ◽

Chain Gene ◽

Alternative Mrna Splicing ◽

A Chain

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Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

Molecular and Cellular Biology ◽

10.1128/mcb.8.2.571 ◽

1988 ◽

Vol 8 (2) ◽

pp. 571-577 ◽

Cited By ~ 90

Author(s):

F Rorsman ◽

M Bywater ◽

T J Knott ◽

J Scott ◽

C Betsholtz

Keyword(s):

Growth Factor ◽

Genomic Dna ◽

Human Platelet ◽

Restriction Endonuclease Analysis ◽

Platelet Derived Growth Factor ◽

Alternative Exon ◽

Chain Gene ◽

Precursor Proteins ◽

Exon Usage ◽

A Chain

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

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