scholarly journals Angiotensin II (ATII)-inducible Platelet-derived Growth Factor A-chain Gene Expression Is p42/44 Extracellular Signal-regulated Kinase-1/2 and Egr-1-dependent and Mediated via the ATII Type 1 but Not Type 2 Receptor

1999 ◽  
Vol 274 (34) ◽  
pp. 23726-23733 ◽  
Author(s):  
Fiona L. Day ◽  
Louise A. Rafty ◽  
Colin N. Chesterman ◽  
Levon M. Khachigian
Keyword(s):  
Gene Expression ◽  
Angiotensin Ii ◽  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal ◽  
A Chain

Platelet-derived growth factor and growth-related genes in rat lung. II. Effect of exposure to 85% O2

1992 ◽  
Vol 262 (2) ◽  
pp. L140-L146 ◽  
Author(s):  
R. N. Han ◽  
S. Buch ◽  
B. A. Freeman ◽  
M. Post ◽  
A. K. Tanswell
Keyword(s):  
Gene Expression ◽  
Cell Division ◽  
Growth Factor ◽  
Receptor Gene ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
Transcription Analysis ◽  
A Chain ◽  
Type Receptor ◽  
Receptor Gene Expression

The expression of platelet-derived growth factor (PDGF), its receptor, and related genes was studied in the lung tissue of rats exposed to air or 85% O2. PDGF-B chain mRNA was increased by 6 days and PDGF B-type receptor mRNA was increased by 4 and 6 days of exposure to 85% O2. Despite a continued increase of cell division, both PDGF-B chain and B-type receptor mRNAs had returned to control values by 14 days of exposure to 85% O2. PDGF-A chain mRNA was unaffected by exposure to 85% O2. Nuclear runoff transcription analysis confirmed increased transcription of PDGF-B chain mRNA, whereas Western blot analysis of lung homogenates suggested consequent increased translation of PDGF-B chain mRNA to PDGF-BB at 7 days of exposure to 85% O2. Combined immunocytochemistry and autoradiography localized PDGF-BB to the major site of cell division, the pulmonary interstitium. We speculate that the early pulmonary fibroblast hyperplasia observed following exposure to 85% O2 is mediated by increased PDGF-B chain gene expression and may also be mediated by changes in PDGF B-type receptor gene expression.

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