scholarly journals Initiation of DNA replication in ColE1 plasmids containing multiple potential origins of replication.

1992 ◽  
Vol 267 (31) ◽  
pp. 22496-22505
Author(s):  
L Martín-Parras ◽  
P Hernández ◽  
M.L. Martínez-Robles ◽  
J.B. Schvartzman
Keyword(s):  
Dna Replication ◽  
Origins Of Replication ◽  
Initiation Of Dna Replication

scholarly journals Identification of the unwinding region in the Clostridioides difficile chromosomal origin of replication

2020 ◽  
Author(s):  
Ana M. Oliveira Paiva ◽  
Erika van Eijk ◽  
Annemieke H. Friggen ◽  
Christoph Weigel ◽  
Wiep Klaas Smits
Keyword(s):  
Dna Replication ◽  
Intergenic Region ◽  
Intestinal Inflammation ◽  
Origin Of Replication ◽  
Chromosomal Origin ◽  
Clostridioides Difficile ◽  
Origins Of Replication ◽  
Origin Region ◽  
Initiation Of Dna Replication

AbstractFaithful DNA replication is crucial for viability of cells across all kingdoms of life. Targeting DNA replication is a viable strategy for inhibition of bacterial pathogens. Clostridioides difficile is an important enteropathogen that causes potentially fatal intestinal inflammation. Knowledge about DNA replication in this organism is limited and no data is available on the very first steps of DNA replication. Here, we use a combination of in silico predictions and in vitro experiments to demonstrate that C. difficile employs a bipartite origin of replication that shows DnaA-dependent melting at oriC2, located in the dnaA-dnaN intergenic region. Analysis of putative origins of replication in different clostridia suggests that the main features of the origin architecture are conserved. This study is the first to characterize aspects of the origin region of C. difficile and contributes to our understanding of the initiation of DNA replication in clostridia.

A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria

2019 ◽  
Vol 16 (3) ◽  
pp. 272-277 ◽  
Author(s):  
Rasmus N. Klitgaard ◽  
Anders Løbner-Olesen
Keyword(s):  
Dna Replication ◽  
High Throughput ◽  
Cyclic Peptide ◽  
Replication Initiation ◽  
False Positive Result ◽  
Over Expression ◽  
Initiator Protein ◽  
Dna Replication Initiation ◽  
Cellular Processes ◽  
Initiation Of Dna Replication

Background:One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited.Materials and Methods:Using various genetic tools, we constructed a novel high throughput, cellbased, fluorescence screen for inhibitors of chromosome replication initiation in bacteria.Results:The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA. We also demonstrated that neither tetracycline nor ciprofloxacin triggers a false positive result. Finally, 400 extracts isolated mainly from filamentous actinomycetes were subjected to the screen.Conclusion:We concluded that the presented screen is applicable for identifying putative inhibitors of DNA replication initiation in a high throughput setup.

scholarly journals Twomcm3Mutations Affect Different Steps in the Initiation of DNA Replication

2002 ◽  
Vol 277 (34) ◽  
pp. 30824-30831 ◽  
Author(s):  
Ming Lei ◽  
Irene H. Cheng ◽  
Louis A. Roberts ◽  
Michael A. McAlear ◽  
Bik K. Tye
Keyword(s):  
Dna Replication ◽  
Initiation Of Dna Replication
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