Evidence for two conformers of the beta subunit of tryptophan synthase in solution

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)50092-2 ◽

1992 ◽

Vol 267 (32) ◽

pp. 23309-23317

Author(s):

S.A. Ahmed ◽

E.W. Miles

Keyword(s):

Beta Subunit ◽

Tryptophan Synthase

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Mechanism of mutual activation of the tryptophan synthase alpha and beta subunits. Analysis of the reaction specificity and substrate-induced inactivation of active site and tunnel mutants of the beta subunit.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54673-1 ◽

1991 ◽

Vol 266 (32) ◽

pp. 21548-21557 ◽

Author(s):

S.A. Ahmed ◽

S.B. Ruvinov ◽

A.M. Kayastha ◽

E.W. Miles

Keyword(s):

Active Site ◽

Beta Subunit ◽

Beta Subunits ◽

Tryptophan Synthase ◽

Reaction Specificity

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An amino acid switch (Gly281–>Arg) within the “hinge” region of the tryptophan synthase beta subunit creates a novel cleavage site for the OmpT protease and selectively diminishes affinity toward a specific monoclonal antibody

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)82420-6 ◽

1993 ◽

Vol 268 (20) ◽

pp. 14912-14920 ◽

Author(s):

G.P. Zhao ◽

R.L. Somerville

Keyword(s):

Monoclonal Antibody ◽

Cleavage Site ◽

Hinge Region ◽

Beta Subunit ◽

Tryptophan Synthase ◽

Specific Monoclonal Antibody

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Evidence that glutamic acid 49 of tryptophan synthase alpha subunit is a catalytic residue. Inactive mutant proteins substituted at position 49 bind ligands and transmit ligand-dependent to the beta subunit.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)68348-6 ◽

1988 ◽

Vol 263 (18) ◽

pp. 8611-8614 ◽

Author(s):

E W Miles ◽

P McPhie ◽

K Yutani

Keyword(s):

Glutamic Acid ◽

Beta Subunit ◽

Alpha Subunit ◽

Catalytic Residue ◽

Tryptophan Synthase ◽

Mutant Proteins

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Phylogeny of metabolic pathways: O-acetylserine sulphydrylase A is homologous to the tryptophan synthase beta subunit

Molecular Microbiology ◽

10.1111/j.1365-2958.1988.tb00089.x ◽

1988 ◽

Vol 2 (6) ◽

pp. 777-783 ◽

Author(s):

S. Levy ◽

A. Danchinl

Keyword(s):

Metabolic Pathways ◽

Beta Subunit ◽

Tryptophan Synthase

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Substitution of glutamic acid 109 by aspartic acid alters the substrate specificity and catalytic activity of the .beta.-subunit in the tryptophan synthase bienzyme complex from Salmonella typhimurium

Biochemistry ◽

10.1021/bi00119a030 ◽

1992 ◽

Vol 31 (4) ◽

pp. 1180-1190 ◽

Author(s):

Peter S. Brzovic ◽

Arvind M. Kayastha ◽

Edith Wilson Miles ◽

Michael F. Dunn

Keyword(s):

Catalytic Activity ◽

Substrate Specificity ◽

Glutamic Acid ◽

Salmonella Typhimurium ◽

Aspartic Acid ◽

Beta Subunit ◽

Tryptophan Synthase

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Synechocystis PCC 6803 Contains a Single Gene for the [beta] Subunit of Tryptophan Synthase with Strong Homology to the trpB Genes of Arabidopsis and Maize (Zea mays L.)

PLANT PHYSIOLOGY ◽

10.1104/pp.104.2.461 ◽

1994 ◽

Vol 104 (2) ◽

pp. 461-466 ◽

Author(s):

G. P. Zhao ◽

R. L. Somerville ◽

P. R. Chitnis

Keyword(s):

Zea Mays ◽

Single Gene ◽

Beta Subunit ◽

Tryptophan Synthase ◽

Synechocystis Pcc 6803 ◽

Strong Homology ◽

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Determination of crystal structure of tryptophan synthase beta subunit from hyperthermophile

Seibutsu Butsuri ◽

10.2142/biophys.40.s29_4 ◽

2000 ◽

Vol 40 (supplement) ◽

pp. S29

Author(s):

Y. Hioki ◽

Y. Yamagata ◽

K. Ogasahara ◽

M. Ishida ◽

S. Kuramitsu ◽

...

Keyword(s):

Crystal Structure ◽

Beta Subunit ◽

Tryptophan Synthase

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A single amino acid switch within the “hinge” region of the tryptophan synthase beta subunit of Escherichia coli that leads to diminished association with alpha subunit and arrested conversion of ESII to product

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)82421-8 ◽

1993 ◽

Vol 268 (20) ◽

pp. 14921-14931 ◽

Author(s):

G.P. Zhao ◽

R.L. Somerville

Keyword(s):

Escherichia Coli ◽

Hinge Region ◽

Beta Subunit ◽

Alpha Subunit ◽

Single Amino Acid ◽

Tryptophan Synthase

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Site-directed mutagenesis of the beta subunit of tryptophan synthase from Salmonella typhimurium. Role of active site glutamic acid 350.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)89492-7 ◽

1991 ◽

Vol 266 (12) ◽

pp. 7618-7625

Author(s):

A M Kayastha ◽

Y Sawa ◽

S Nagata ◽

E W Miles

Keyword(s):

Glutamic Acid ◽

Salmonella Typhimurium ◽

Active Site ◽

Beta Subunit ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Tryptophan Synthase

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A novel intersubunit repair mechanism in the tryptophan synthase alpha 2 beta 2 complex. Critical role of the beta subunit lysine 167 in intersubunit communication.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)41523-2 ◽

1993 ◽

Vol 268 (30) ◽

pp. 22269-22272 ◽

Author(s):

X.J. Yang ◽

E.W. Miles

Keyword(s):

Critical Role ◽

Beta Subunit ◽

Tryptophan Synthase ◽

Repair Mechanism ◽

Beta 2 ◽

Intersubunit Communication

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