scholarly journals An amino acid switch (Gly281–>Arg) within the “hinge” region of the tryptophan synthase beta subunit creates a novel cleavage site for the OmpT protease and selectively diminishes affinity toward a specific monoclonal antibody

1993 ◽  
Vol 268 (20) ◽  
pp. 14912-14920 ◽  
Author(s):  
G.P. Zhao ◽  
R.L. Somerville
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Cleavage Site ◽  
Hinge Region ◽  
Beta Subunit ◽  
Tryptophan Synthase ◽  
Specific Monoclonal Antibody

Effect of D-Amino Acid Substitution in a Mucin 2 Epitope on Mucin-Specific Monoclonal Antibody Recognition

2000 ◽  
Vol 378 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Katalin Uray ◽  
Judit Kajtár ◽  
Elemér Vass ◽  
Michael R. Price ◽  
Miklós Hollósi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Amino Acid Substitution ◽  
Specific Monoclonal Antibody ◽  
Antibody Recognition ◽  
Mucin 2

Gliadin as a Measure of Gluten in Foods Containing Wheat, Rye, and Barley—Enzyme Immunoassay Method Based on a Specific Monoclonal Antibody to the Potentially Celiac Toxic Amino Acid Prolamin Sequences: Collaborative Study

2012 ◽  
Vol 95 (4) ◽  
pp. 1118-1124 ◽  
Author(s):  
Ulrike Immer ◽  
Sigrid Haas-Lauterbach ◽  
V Cerne ◽  
F Chirdo ◽  
J de Sadeleer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Working Group ◽  
Collaborative Study ◽  
Poor Performance ◽  
Elisa Test ◽  
Specific Monoclonal Antibody ◽  
Negative Sample ◽  
Heat Treated ◽  
Ppm Level

Abstract The Working Group on Prolamin Analysis and Toxicity (WGPAT) organized a collaborative study to confirm whether the two R5 antibody-based ELISA test kits are able to detect gliadin in the lower mg/kg (ppm) level. Twenty laboratories investigated 12 blind-coded samples, spiked and naturally contaminated, to show the possibility of determining traces of gliadin in heat-treated or nonheat-treated foods by ELISA. It was shown that very small amounts of gliadin (below 100 ppm) could be detected by ELISA with a reproducibility RSDR (37%) and a repeatability RSDr (27%) common for ELISA under these conditions. The recovery of gliadin from the spiked samples was between 84 and 109%, based on the results of all laboratories, including those with poor performance. No false positives were found by the method (P ≤ 0.05), but one negative sample was contaminated during the bakery process. It is recommended that the method be accepted by AOAC as Official First Action.

Isolation of an atypical pigeon paramyxovirus type 1 in Poland

2011 ◽  
Vol 14 (1) ◽  
pp. 141-143 ◽  
Author(s):  
K. Śmietanka ◽  
Z. Minta
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cleavage Site ◽  
Antibody Binding ◽  
Binding Pattern ◽  
Virulent Strains ◽  
Pigeon Paramyxovirus Type 1 ◽  
Monoclonal Antibody Binding

Isolation of an atypical pigeon paramyxovirus type 1 in Poland In this study, a pigeon paramyxovirus type 1 (PPMV-1) isolated from a flock of ornamental pigeons in Poland in 2010 is described. The PPMV-1/Poland/H2/10 isolate showed the amino acid sequence at the cleavage site of F2/F1 112KRQKRF117 i.e. typical of virulent strains. Despite having the monoclonal antibody binding pattern typical of pigeon variants PPMV-1 (antigenic group "P"), the Polish isolate clustered into genetic sublineage 4a, which is usually associated with PMV-1 isolated from poultry.

Sign in / Sign up

Export Citation Format

Share Document