scholarly journals BIOSYNTHESIS OF ITACONIC ACID IN ASPERGILLUS TERREUS

1957 ◽  
Vol 226 (2) ◽  
pp. 689-701
Author(s):  
Ronald Bentley ◽  
Clara P. Thiessen
Keyword(s):  
Itaconic Acid ◽  
Aspergillus Terreus

scholarly journals An Optimized Ustilago Chassis for Itaconic Acid Production at Theoretical Maximal Yield

2020 ◽  
Author(s):  
J. Becker ◽  
H. Hosseinpour Tehrani ◽  
P. Ernst ◽  
L. M. Blank ◽  
N. Wierckx
Keyword(s):  
Itaconic Acid ◽  
Acid Production ◽  
Aspergillus Terreus ◽  
Lipid Production ◽  
Ustilago Maydis ◽  
Product Formation ◽  
Fed Batch ◽  
Maximal Yield ◽  
Morphology Engineering ◽  
Continuous Feed

Ustilago maydis, member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing Ustilago strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through fuz7 deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster ria1 and the mitochondrial tricarboxylate transporter encoded by mttA from Aspergillus terreus. In this study, itaconate production was further optimized by consolidating these different optimizations into one strain. The combined modifications resulted in itaconic acid production at theoretical maximal yield, which was achieved under biotechnologically relevant fed-batch fermentations with continuous feed.

scholarly journals Production of Itaconic Acid from Jatropha curcas Seed Cake by Aspergillus terreus

2013 ◽  
Vol 5 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Amina M. AHMED EL-IMAM ◽  
Muinat O. KAZEEM ◽  
Mutiat B. ODEBISI ◽  
Mushaffa A. OKE ◽  
Azeezat O. ABIDOYE
Keyword(s):  
Itaconic Acid ◽  
Jatropha Curcas ◽  
Substrate Concentration ◽  
Aspergillus Terreus ◽  
Maximum Yield ◽  
Inoculum Size ◽  
Daily Maximum ◽  
Dilute Acid Hydrolysis ◽  
Seed Cake ◽  
Jatropha Seed

Submerged substrate fermentation of Jatropha seed cake, a by-product of oil extraction from Jatropha curcas seed was carried out using Aspergillus terreus for the production of itaconic acid. The Jatropha seed cake was initially converted into fermentable sugars by dilute acid hydrolysis using 50% sulphuric acid. The rate of hydrolysis was 1.04 gL-1. The fermentation process was carried out at room temperature, agitation of 400 rpm and three physico-chemical parameters (pH, inoculum size and substrate concentration) were varied. Itaconic acid and glucose assays were carried out by spectrophotometry and Dinitrosalicylic acid methods respectively daily. Maximum yield of itaconic acid was 48.70 gL-1 at 5 ml of inoculum size, 50 g substrate concentration and pH 1.5. The residual glucose concentration increased for the first two days of fermentation after which it began to decrease as the itaconic acid concentration increased. The least concentration of itaconic acid observed was 6.00 gL-1, obtained after 24 hours of fermentation with 4 ml inoculum size, 50 g substrate concentration and at pH 1.5. The findings of this work indicate that Jatropha curcas seed cake is a suitable substrate for itaconic acid production.

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