Quantitative detection of messenger RNA by solution hybridization and enzyme immunoassay.

Laboratory diagnosis of blastomycosis relies on a combination of methods, including antigen detection. We assessed performance of analyte specific reagents from Gotham Biotech (Portland, ME) for quantitative detection of Blastomyces dermatitidis galactomannan (GM) in urine using an enzyme immunoassay (EIA), compared to the Blastomyces quantitative EIA from MiraVista Diagnostics (Indianapolis, IN). Residual urine from 232 unique patients previously tested by the MiraVista assay were evaluated using the Gotham EIA, which showed 97.4% (74/76), 100% (156/156), and 99.1% (230/232) positive, negative, and overall agreement, respectively. Correlation between the quantitative B. dermatitidis antigen levels by the Gotham and MiraVista EIAs was low (R 2 =0.20). Medical records were available for 36 of the 232 patients, among whom four had confirmed blastomycosis and both the Gotham and MiraVista EIAs were positive. Nine of these patients had histoplasmosis, and the Gotham and MiraVista EIAs yielded negative results in 44.4% (4/9) and 22.2% (2/9) of cases, respectively. Both assays were negative in the remaining 23 patients. Post-laboratory implementation of the Gotham EIA, chart reviews were performed on the first 50 unique patients (51 samples) tested by the assay in our hospital. Among these, 3/50 (6%) samples were positive by the Gotham EIA; two samples from a patient with culture-confirmed blastomycosis and one from a patient with histoplasmosis (also positive by the MiraVista Blastomyces EIA). All remaining patients were negative by the Gotham EIA and had alternative diagnoses. Our findings show comparable performance between the Gotham and MiraVista quantitative EIAs for detection of B. dermatitidis GM in urine.

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Quantitative detection of EP3-II, III and VI messenger RNA in gravid and non-gravid human myometrium using real-time RT-PCR

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.1080/14767050802353549 ◽

2009 ◽

Vol 22 (1) ◽

pp. 59-64

Author(s):

Richard H. Lee ◽

T. Murphy Goodwin ◽

Wangrong Yang ◽

Aimin Li ◽

Melissa L. Wilson ◽

...

Keyword(s):

Real Time ◽

Messenger Rna ◽

Human Myometrium ◽

Quantitative Detection ◽

Rt Pcr

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A competitive enzyme immunoassay for the quantitative detection of cocaine from banknotes and latent fingermarks

Forensic Science International ◽

10.1016/j.forsciint.2015.02.008 ◽

2015 ◽

Vol 250 ◽

pp. 1-7 ◽

Cited By ~ 23

Author(s):

Susan van der Heide ◽

Paula Garcia Calavia ◽

Sheila Hardwick ◽

Simon Hudson ◽

Kim Wolff ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Quantitative Detection

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Prevalence rate of Helicobacter pylori infection among population of Ryazan region

I P Pavlov Russian Medical Biological Herald ◽

10.23888/pavlovj201927135-40 ◽

2019 ◽

Vol 27 (1) ◽

pp. 35-40

Author(s):

Tatyana V. Zhestkova ◽

Mikhail A. Butov ◽

Yulian Yu. Lymar ◽

Sergey V. Papkov

Keyword(s):

Helicobacter Pylori ◽

Enzyme Immunoassay ◽

Serological Test ◽

Adult Population ◽

Test System ◽

Helicobacter Pylori Infection ◽

Quantitative Detection ◽

Serological Tests ◽

H Pylori

Aim. Determination of the prevalence of Helicobacter pylori (H. pylori) infection among the population of the Ryazan region. Materials and Methods. 833 individuals (809 adults and 24 children) were examined for presence of IgG class antibodies using the enzyme immunoassay (2017-2018). The criteria for inclusion into the study were: a desire of a patient to undergo examination for the presence of antibodies to H. pylori in blood. Criteria for exclusion: past treatment for helicobacteriosis. The presence of helicobacteriosis was determined by enzyme immunoassay for quantitative detection of IgG class antibodies (anti-H. pylori IgG) using BCM Diagnostics Helicobacter pylori IgG (USA) test system and for qualitative determination of IgG antibodies to H. pylori in blood serum on IMMULITE 2000 (Germany; test IMMULITE 2000 H. pylori IgG). Sensitivity of the used test systems was 95.0%, specificity 98.0%. Results. High contamination of adult residents of Ryazan with H. pylori 65.6% was found (70.6% of males, 64.4% of females). Prevalence of H. pylori infection among adults in 2017 was 64.4% and in 2018 70.2%, however, the observed increase in the number of infected individuals was not statistically significant (p0.05). The highest prevalence of H. pylori infection was observed in individuals 40 years of age (67.2%). Gender-related differences in the prevalence of Helicobacter pylori infection were revealed in individuals of 40 years and older. H. pylori infection in males of 40 years was 75.2%, against 65.5% in females of the same age (p0.05). In children of 4-16 years, the share of individuals with positive serological test with anti-H. pylori IgG reached 20.8%. All H. pylori infected children were above 9 years of age. Individuals with positive serological tests received consultation of a gastroenterologist, and on indications underwent additional examination with administration of eradication treatment. In patients with indefinite results the examination was repeated after a week and/or the presence of H. pylori antigen in feces was determined. Conclusion. The data obtained indicate a high level of infection with H. pylori in the adult population in the Ryazan region 65.6%. The incidence of detection of anti-H. pylori IgG in the population was maximal in individuals 40 years (67.2%).

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Striational Autoantibodies: Quantitative Detection by Enzyme Immunoassay in Myasthenia Gravis, Thymoma, and Recipients of d-Penicillamine or Allogeneic Bone Marrow

Mayo Clinic Proceedings ◽

10.1016/s0025-6196(12)65645-6 ◽

1988 ◽

Vol 63 (5) ◽

pp. 474-481 ◽

Cited By ~ 40

Author(s):

NADA CIKES ◽

MARIKO Y. MOMOI ◽

CAROL L. WILLIAMS ◽

FRANK M. HOWARD ◽

H. CLARK HOAGLAND ◽

...

Keyword(s):

Bone Marrow ◽

Myasthenia Gravis ◽

Enzyme Immunoassay ◽

Allogeneic Bone Marrow ◽

Quantitative Detection ◽

Allogeneic Bone

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High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166944 ◽

1996 ◽

Vol 54 ◽

pp. 896-897

Author(s):

G. W. Hacker ◽

I. Zehbe ◽

J. Hainfeld ◽

A.-H. Graf ◽

C. Hauser-Kronberger ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Messenger Rna ◽

High Performance ◽

Detection System ◽

Direct Methods ◽

Genetic Alterations ◽

Chain Reaction ◽

Protein Encoding ◽

Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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