Stimulation of the uptake of soluble proteins into isolated HeLa nuclei by pancreatic deoxyribonuclease

The influence of optimal concentrations 5 x 10-6M - 10-6M of 11-deoxycorticosterone (mineralocorticoid) and prednisolone (glucocorticoid) on the growth (fresh and dry weight) and content of soluble proteins, reducing sugars and nucleic acids in the green alga Chlorella vulgaris (Chlorophyceae). Both corticosteroids at concentration 5 x 10-6M were most strongly active metabolically between the 5th-15th day of the cultivation and this probably was caused by their chemical biotransformation. The applied corticosteroids induced the strongest stimulative effect on the content of soluble proteins in the range of 167-196% and reducing sugars (233-275%) when compared to the control (100%). Prednisolone showed lower stimulative activity on the content of proteins. But 11-deoxycorticosterone showed weaker stimulation of on the content of sugar. Both of the corticosteroids showed a stimulating or inhibitory influence upon the content of nucleic acids in C. vulgaris cells without regard to the concentration.

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Insulin stimulates synthesis of soluble proteins in isolated rat hepatocytes

Biochemical Journal ◽

10.1042/bj1900615 ◽

1980 ◽

Vol 190 (3) ◽

pp. 615-619 ◽

Cited By ~ 5

Author(s):

R L Clark ◽

R J Hansen

Keyword(s):

Protein Synthesis ◽

Rat Hepatocytes ◽

Isolated Hepatocytes ◽

Cellular Protein ◽

Soluble Proteins ◽

Leucine Incorporation ◽

Isolated Rat Hepatocytes ◽

Hepatic Protein Synthesis ◽

Isolated Rat ◽

Stimulation Of

The incorporation of [3H]leucine into soluble cellular protein was measured in isolated hepatocytes at extracellular leucine concentrations ranging from 0.15 to 20.0 mM. Insulin caused a 12—15% stimulation of [3H]leucine incorporation in the presence of high extracellular leucine concentrations. It is concluded that insulin causes a small but significant increase in the rate of hepatic protein synthesis.

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Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)77942-3 ◽

1988 ◽

Vol 263 (4) ◽

pp. 1768-1772

Author(s):

S C Tsai ◽

M Noda ◽

R Adamik ◽

P P Chang ◽

H C Chen ◽

...

Keyword(s):

Bovine Brain ◽

Soluble Proteins ◽

Enzymatic Activities ◽

Stimulation Of

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Epidermal growth factor (EGF) stimulation of cultured brain cells. II. Increased production of extracellular soluble proteins

Developmental Brain Research ◽

10.1016/0165-3806(83)90223-7 ◽

1983 ◽

Vol 11 (2) ◽

pp. 253-260 ◽

Cited By ~ 27

Author(s):

Beatrice Guentert-Lauber ◽

Paul Honegger

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Soluble Proteins ◽

Brain Cells ◽

Epidermal Growth ◽

Stimulation Of

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Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066917 ◽

1971 ◽

Vol 29 ◽

pp. 570-571

Author(s):

E. A. Elfont ◽

R. B. Tobin ◽

D. G. Colton ◽

M. A. Mehlman

Keyword(s):

Chemical Composition ◽

Rat Liver ◽

Future Study ◽

Mode Of Action ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Effective Inhibitor ◽

Biochemical Effects ◽

Glycerophosphate Dehydrogenase ◽

Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

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Gap junctions in the prothoracic glands of the tobacco hornworm, Manduca sexta

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123933 ◽

1992 ◽

Vol 50 (1) ◽

pp. 706-707

Author(s):

Ji-da Dai ◽

M. Joseph Costello ◽

Lawrence I. Gilbert

Keyword(s):

Gap Junctions ◽

Small Molecules ◽

Manduca Sexta ◽

Freeze Fracture ◽

Cell Communication ◽

Cellular Level ◽

Thin Sections ◽

Prothoracic Glands ◽

Polyhydroxylated Steroids ◽

Stimulation Of

Insect molting and metamorphosis are elicited by a class of polyhydroxylated steroids, ecdysteroids, that originate in the prothoracic glands (PGs). Prothoracicotropic hormone stimulation of steroidogenesis by the PGs at the cellular level involves both calcium and cAMP. Cell-to-cell communication mediated by gap junctions may play a key role in regulating signal transduction by controlling the transmission of small molecules and ions between adjacent cells. This is the first report of gap junctions in the PGs, the evidence obtained by means of SEM, thin sections and freeze-fracture replicas.

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