THE RÔLE OF ACETIC ACID IN THE BIOSYNTHESIS OF HEME

In the present paper we describe a new carboxylic acid transporter in Escherichia coli encoded by the gene yaaH. In contrast to what had been described for other YaaH family members, the E. coli transporter is highly specific for acetic acid (a monocarboxylate) and for succinic acid (a dicarboxylate), with affinity constants at pH 6.0 of 1.24±0.13 mM for acetic acid and 1.18±0.10 mM for succinic acid. In glucose-grown cells the ΔyaaH mutant is compromised for the uptake of both labelled acetic and succinic acids. YaaH, together with ActP, described previously as an acetate transporter, affect the use of acetic acid as sole carbon and energy source. Both genes have to be deleted simultaneously to abolish acetate transport. The uptake of acetate and succinate was restored when yaaH was expressed in trans in ΔyaaH ΔactP cells. We also demonstrate the critical role of YaaH amino acid residues Leu131 and Ala164 on the enhanced ability to transport lactate. Owing to its functional role in acetate and succinate uptake we propose its assignment as SatP: the Succinate–Acetate Transporter Protein.

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The role of biomass in polyethylenimine-coated chitosan/bacterial biomass composite biosorbent fiber for removal of Ru from acetic acid waste solution

Bioresource Technology ◽

10.1016/j.biortech.2014.01.082 ◽

2014 ◽

Vol 160 ◽

pp. 93-97 ◽

Cited By ~ 29

Author(s):

Sung Wook Won ◽

In Seob Kwak ◽

Yeoung-Sang Yun

Keyword(s):

Acetic Acid ◽

Bacterial Biomass ◽

Waste Solution ◽

Acid Waste

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The Role of Boron in Plant Growth: IV. INTERRELATIONSHIPS BETWEEN BORON AND INDOL-3YL-ACETIC ACID IN THE METABOLISM OF BEAN RADICLES

Journal of Experimental Botany ◽

10.1093/jxb/19.2.295 ◽

1968 ◽

Vol 19 (2) ◽

pp. 295-308 ◽

Cited By ~ 42

Author(s):

L. COKE ◽

W. J. WHITTINGTON

Keyword(s):

Acetic Acid ◽

Plant Growth

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The role of acidity profile in the nanotubular growth of polyaniline

Chemical Papers ◽

10.2478/s11696-009-0101-z ◽

2010 ◽

Vol 64 (1) ◽

Cited By ~ 26

Author(s):

Elena Konyushenko ◽

Miroslava Trchová ◽

Jaroslav Stejskal ◽

Irina Sapurina

Keyword(s):

Acetic Acid ◽

Sulfuric Acid ◽

Reaction Temperature ◽

Subsequent Growth ◽

Subsequent Oxidation ◽

Ammonium Peroxydisulfate ◽

Aniline Oligomers ◽

Positive Effect ◽

Granular Morphology

AbstractConditions of polyaniline (PANI) nanotubes preparation were analyzed. Aniline was oxidized with ammonium peroxydisulfate in 0.4 M acetic acid. There are two subsequent oxidation steps and the products were collected after each of them. At pH > 3, neutral aniline molecules are oxidized to non-conducting aniline oligomers. These produce templates for the subsequent growth of PANI nanotubes, which takes place preferably at pH 2–3. At pH < 2, granular morphology of the conducting PANI is obtained. High final acidity of the medium should be avoided in the preparation of nanotubes, e.g., by reducing the amount of sulfuric acid which is a by-product. Reduction of the peroxydisulfate-to-aniline mole ratio was tested for this purpose in the present study. Lowering of the reaction temperature from 20°C to −4°C had a positive effect on the formation of nanotubes.

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Kinetics and Mechanism of Hydrolysis of Acetylthiocholine by Butyrylcholine Esterase

Zeitschrift für Naturforschung C ◽

10.1515/znc-2002-11-1221 ◽

2002 ◽

Vol 57 (11-12) ◽

pp. 1072-1077 ◽

Cited By ~ 4

Author(s):

Karel Komers ◽

Alexandr Čegan ◽

Marek Link

Keyword(s):

Acetic Acid ◽

Kinetics And Mechanism ◽

Kinetics Parameters ◽

Mechanism Of Hydrolysis ◽

Ph Stat ◽

Stat Method ◽

Ellman’S Method ◽

Hydrolysis Of

Kinetics and mechanism of hydrolysis of acetylthiocholine by the enzyme butyrylcholine esterase was studied. The spectrophotometric Ellman’s method and potentiometric pH-stat method were used for continuous determination of the actual concentration of the products thiocholine and acetic acid in the reaction mixture. The validity of the Michaelis-Menten (Briggs-Haldane) equation in the whole course of the reaction under used conditions was proved. The corresponding kinetics parameters (Vm and KM) were calculated from the obtained dependences of concentration of thiocholine or acetic acid vs. time and compared. From this comparison the deciding kinetic role of the step producing thiocholine was derived. The values of initial molar concentration of the enzyme and of the rate constants of the kinetic model were estimated.

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Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.44.38 ◽

2015 ◽

Vol 44 ◽

pp. 38-44 ◽

Cited By ~ 1

Author(s):

H. Sandhya ◽

Rao Srinath

Keyword(s):

Acetic Acid ◽

Growth Regulators ◽

Callus Induction ◽

Basal Medium ◽

Direct Regeneration ◽

Naphthalene Acetic Acid ◽

Stem Explants ◽

Dichlorophenoxy Acetic Acid ◽

Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

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