Separation and determination of nanomole amounts of some purines, uric acid and uric acid riboside by two-dimensional paper chromatography

1974 ◽  
Vol 100 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Maria Krzyžanowska ◽  
W. Niemierko
Keyword(s):  
Uric Acid ◽  
Paper Chromatography ◽  
Two Dimensional

Two-Dimensional Paper Chromatographic Determination of Amino Acids in Urine

1969 ◽  
Vol 15 (10) ◽  
pp. 1011-1016 ◽  
Author(s):  
Carmine J Spinella
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Inborn Errors Of Metabolism ◽  
Chromatographic Determination ◽  
Two Dimensional ◽  
Standard Mixture ◽  
Inborn Errors ◽  
Chromatography Method ◽  
Amino Aciduria

Abstract In an attempt to screen children for amino-aciduria related to various inborn errors of metabolism, a two-dimensional paper chromatography method was developed. This method requires no expensive equipment, resolves a standard mixture of 17 α-amino acids, is consistently reproducible, and requires less than 7 hr from start to visualization of the chromatogram.

Comparison of Methods for Determination of Lysine in Cereals

1963 ◽  
Vol 46 (3) ◽  
pp. 399-405
Author(s):  
Y Pomeranz ◽  
B S Miller
Keyword(s):  
Ion Exchange ◽  
Paper Chromatography ◽  
Microbiological Assay ◽  
Two Dimensional ◽  
Comparison Of Methods ◽  
Microbiological Method ◽  
Exchange Method ◽  
Wheat Products ◽  
Ion Exchange Method

Abstract The lysine contents of 10 milled wheat products and cereal foods were determined by microbiological, enzymatic, two-dimensional paper chromatographic, and ion exchange chromatographic methods. The assay of lysine by two-dimensional paper chromatography produced very low results. The results from the microbiological method were comparable to those by the ion exchange method. The decarboxylase method consistently gave low results which averaged 82% of those obtained by the microbiological assay. Possible reasons for these low results are presented and discussed.

Determination of Compounds Related to Vanillin in Vanilla Extracts

1964 ◽  
Vol 47 (5) ◽  
pp. 808-815 ◽  
Author(s):  
D Morison Smith
Keyword(s):  
Paper Chromatography ◽  
Vanillic Acid ◽  
Hydroxybenzoic Acid ◽  
Two Dimensional ◽  
Vanilla Planifolia ◽  
Colored Spot

Abstract A method has been developed for the estimation of the quantities of vanillic acid, p-hydroxybenzoic acid, and p-hydroxybenzaldehyde in vanilla extracts. This method separates these compounds from each other and from vanillin by two-dimensional paper chromatography. An estimate of the quantity of each of these compounds is obtained by measuring the area of the colored spot produced on reacting the compounds with chromogenic reagents. The vanillin content was also determined by two different methods. Vanilla planifolia was found to have an unidentified phenolic component not present in V. tahitensis. The Tahiti vanillas contained over twice as much p-hydroxybenzoic acid as any other vanilla extracts.

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