THE IDENTIFICATION AND DETERMINATION OF SOME PHENOLIC ACIDS IN URINE USING TWO-DIMENSIONAL PAPER CHROMATOGRAPHY

Abstract In an attempt to screen children for amino-aciduria related to various inborn errors of metabolism, a two-dimensional paper chromatography method was developed. This method requires no expensive equipment, resolves a standard mixture of 17 α-amino acids, is consistently reproducible, and requires less than 7 hr from start to visualization of the chromatogram.

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Comparison of Methods for Determination of Lysine in Cereals

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/46.3.399 ◽

1963 ◽

Vol 46 (3) ◽

pp. 399-405

Author(s):

Y Pomeranz ◽

B S Miller

Keyword(s):

Ion Exchange ◽

Paper Chromatography ◽

Microbiological Assay ◽

Two Dimensional ◽

Comparison Of Methods ◽

Microbiological Method ◽

Exchange Method ◽

Wheat Products ◽

Ion Exchange Method

Abstract The lysine contents of 10 milled wheat products and cereal foods were determined by microbiological, enzymatic, two-dimensional paper chromatographic, and ion exchange chromatographic methods. The assay of lysine by two-dimensional paper chromatography produced very low results. The results from the microbiological method were comparable to those by the ion exchange method. The decarboxylase method consistently gave low results which averaged 82% of those obtained by the microbiological assay. Possible reasons for these low results are presented and discussed.

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Determination of Compounds Related to Vanillin in Vanilla Extracts

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/47.5.808 ◽

1964 ◽

Vol 47 (5) ◽

pp. 808-815 ◽

Cited By ~ 4

Author(s):

D Morison Smith

Keyword(s):

Paper Chromatography ◽

Vanillic Acid ◽

Hydroxybenzoic Acid ◽

Two Dimensional ◽

Vanilla Planifolia ◽

Colored Spot

Abstract A method has been developed for the estimation of the quantities of vanillic acid, p-hydroxybenzoic acid, and p-hydroxybenzaldehyde in vanilla extracts. This method separates these compounds from each other and from vanillin by two-dimensional paper chromatography. An estimate of the quantity of each of these compounds is obtained by measuring the area of the colored spot produced on reacting the compounds with chromogenic reagents. The vanillin content was also determined by two different methods. Vanilla planifolia was found to have an unidentified phenolic component not present in V. tahitensis. The Tahiti vanillas contained over twice as much p-hydroxybenzoic acid as any other vanilla extracts.

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Phenolic acids of native species of the Rosa L. genus in Poland

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1979.027 ◽

2015 ◽

Vol 48 (2) ◽

pp. 327-336 ◽

Cited By ~ 5

Author(s):

W. Krzaczek ◽

T. Krzaczek

Keyword(s):

Gallic Acid ◽

Phenolic Acids ◽

Paper Chromatography ◽

Native Species ◽

Two Dimensional ◽

Common Occurrence ◽

Rosa Rubiginosa ◽

Isoferulic Acid ◽

The Common ◽

Salicylic Acids

Phenolic acids were identified in the leaves of 23 species of native roses and their quantitative proportions were determined by the method of two-dimensional paper chromatography. The common occurrence of ellagic and gallic acid in roses was confirmed and so was the regular presence of protocatechuic, caffeic, gentisic, p-hydroxybenzoic, p-hydroxyphenylacetic, p-cumaric, syringic, vanillic, ferulic and salicylic acids. A small amount of isoferulic acid was noted only in Rosa gizellae Borb. and R. eglanteria L. var. comosa (Ripart) Du Mortier. Homoprotocatechuic acid was revealed only in the species of the Rosa rubiginosa and Rosa tomentosa groups from the Caninae section. The taxonomically limited occurrence of some phenolic acids points to the systematic differentiating value of the latter within one genus.

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