Determination of pheophorbide a, pyropheophorbide a and phytol

1983 ◽  
Vol 280 ◽  
pp. 188-193 ◽  
Author(s):  
Yuiko Takeda ◽  
Yukio Saito ◽  
Mitsuru Uchiyama
Keyword(s):  
Pheophorbide A ◽  
Pyropheophorbide A

scholarly journals HPLC Determination of Pheophorbide a and Pyropheophorbide a in Dried Laver Product Implicated in Food Poisoning

2005 ◽  
Vol 46 (2) ◽  
pp. 45-48 ◽  
Author(s):  
Deng-Fwu HWANG ◽  
Yu-Shia TSAI ◽  
Shin-Shoug CHOU ◽  
Shiu-Mei LIU ◽  
Jiunn-Tzong WU ◽  
...  
Keyword(s):  
Food Poisoning ◽  
Hplc Determination ◽  
Pheophorbide A ◽  
Dried Laver ◽  
Pyropheophorbide A

scholarly journals Development of a Solid-Phase Extraction Method for Determination of Pheophorbide a and Pyropheophorbide a in Health Foods by Liquid Chromatography

2004 ◽  
Vol 87 (4) ◽  
pp. 937-942 ◽  
Author(s):  
Harumi Oshima ◽  
Eiji Ueno ◽  
Isao Saito ◽  
Hiroshi Matsumoto
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Reversed Phase ◽  
Official Method ◽  
Phase Extraction ◽  
Pheophorbide A ◽  
Significant Difference ◽  
Spe Method

Abstract A simple solid-phase extraction (SPE) method was developed for the liquid chromatography (LC) determination of pheophorbide (Phor) a and pyropheophorbide (Pyro) a in health foods such as chlorella, spirulina, etc. The food sample was extracted with 85% (v/v) acetone. The extract was acidified with hydrochloric acid and loaded on a C18 cartridge. After washing with water, Phor a and Pyro a were eluted with the LC mobile phase. Phor a and Pyro a were separated by isocratic reversed-phase LC and quantitated by fluorescence detection. The recoveries for spiked samples of chlorella and the extract were 87.1–102.0%. Commercial health foods (chlorella, spirulina, aloe, kale, Jews mallow, and green tea leaves) were analyzed using the SPE method. The values found for Phor a and Pyro a ranged from 2 to 788 μg/g and from <1 to 24 μg/g, respectively. There was no significant difference between the SPE method and the official method in Japan (spectrophotometry after liquid–liquid extraction). The advantages of the SPE method are the short extraction times, lack of emulsions, and reduced consumption of organic solvents compared with the official method in Japan. The SPE method is considered to be useful for the screening of Phor a and Pyro a in health foods.

scholarly journals Determination of pheophorbide a and methylpheophorbide a by fluorescence high performance liquid chromatography: Availability of narrow baseline method.

1986 ◽  
Vol 2 (1) ◽  
pp. 67-69 ◽  
Author(s):  
Yoshiaki YOSHITAKE ◽  
Kikuko KAKIUCHI ◽  
Kiyotoshi MORISHIGE ◽  
Shiro GOHDA ◽  
Yasuharu NISHIKAWA ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pheophorbide A ◽  
Baseline Method

Microbial Formation of Pheophorbide a and Pyropheophorbide a in “Takana” during Brining and Fermentation

1989 ◽  
Vol 30 (3) ◽  
pp. 228-232_1 ◽  
Author(s):  
Yuiko TAKEDA ◽  
Hirotaka KONUMA ◽  
Sadao UCHIYAMA ◽  
Yukio SAITO
Keyword(s):  
Pheophorbide A ◽  
Pyropheophorbide A

Potent virucidal effect of pheophorbide a and pyropheophorbide a on enveloped viruses

2010 ◽  
Vol 65 (1) ◽  
pp. 229-233 ◽  
Author(s):  
Lamjed Bouslama ◽  
Kyoko Hayashi ◽  
Jung-Bum Lee ◽  
Abdelwahed Ghorbel ◽  
Toshimitsu Hayashi
Keyword(s):  
Pheophorbide A ◽  
Enveloped Viruses ◽  
Virucidal Effect ◽  
Pyropheophorbide A

scholarly journals Effects of Chlorophyll-Derived Efflux Pump Inhibitor Pheophorbide a and Pyropheophorbide a on Growth and Macrolide Antibiotic Resistance of Indicator and Anaerobic Swine Manure Bacteria

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Mareike Kraatz ◽  
Terence R. Whitehead ◽  
Michael A. Cotta ◽  
Mark A. Berhow ◽  
Mark A. Rasmussen
Keyword(s):  
Bacterial Resistance ◽  
Efflux Pump ◽  
Growth Parameters ◽  
Swine Manure ◽  
Efflux Pumps ◽  
Resistant Bacteria ◽  
Pheophorbide A ◽  
Efflux Pump Inhibitor ◽  
E Coli ◽  
Pyropheophorbide A

Natural plant compounds, such as the chlorophyll a catabolites pheophorbide a (php) and pyropheophorbide a (pyp), are potentially active in the gastrointestinal tracts and manure of livestock as antimicrobial resistance-modifying agents through inhibition of bacterial efflux pumps. To investigate whether php, a known efflux pump inhibitor, and pyp influence bacterial resistance, we determined their long-term effects on the MICs of erythromycin for reference strains of clinically relevant indicator bacteria with macrolide or multidrug resistance efflux pumps. Pyp reduced the final MIC endpoint for Staphylococcus (S.) aureus and Escherichia (E.) coli by up to 1536 and 1024 μg erythromycin mL−1 or 1.4- and 1.2-fold, respectively. Estimation of growth parameters of S. aureus revealed that pyp exerted an intrinsic inhibitory effect under anaerobic conditions and was synergistically active, thereby potentiating the effect of erythromycin and partially reversing high-level erythromycin resistance. Anaerobe colony counts of total and erythromycin-resistant bacteria from stored swine manure samples tended to be lower in the presence of pyp. Tylosin, php, and pyp were not detectable by HPLC in the manure or medium. This is the first study showing that pyp affects growth and the level of sensitivity to erythromycin of S. aureus, E. coli, and anaerobic manure bacteria.

The quantitation of pheophorbide a, pyropheophorbide a, and their esters

1969 ◽  
Vol 47 (7) ◽  
pp. 721-724 ◽  
Author(s):  
D. W. A. Roberts
Keyword(s):  
Experimental Error ◽  
Pheophorbide A ◽  
Pheophytin A ◽  
Extinction Coefficients ◽  
Pyropheophorbide A ◽  
Molar Extinction Coefficients

The molar extinction coefficients of pheophorbide a, methyl pheophorbide a, and pheophytin a are identical within experimental error. A similar situation holds for pyropheophorbide a and its methyl ester.Pheophorbide a and pyropheophorbide a are adsorbed on glass from solution in ether and chloroform but not from solution in acetone or dioxane.It is recommended acetone be used as the solvent for the quantitation of pheophorbide a and pyropheophorbide a.

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