Specialized thin-layer chromatographic system for some common drug identification problems

1975 ◽  
Vol 106 (2) ◽  
pp. 485-489 ◽  
Author(s):  
P.A.F. Franitis ◽  
A. Stolman
Keyword(s):  
Thin Layer ◽  
Identification Problems ◽  
Chromatographic System ◽  
Drug Identification ◽  
Common Drug

Determination of Dimetridazole and Ipronidazole in Feeds at Cross-Contamination Levels

1988 ◽  
Vol 71 (3) ◽  
pp. 474-477 ◽  
Author(s):  
Duane D Hughes
Keyword(s):  
Thin Layer ◽  
Rapid Method ◽  
Aqueous Phase ◽  
Borate Buffer ◽  
Cross Contamination ◽  
Chromatographic System ◽  
Arsanilic Acid ◽  
Contamination Levels ◽  
Benzene Extract

Abstract A rapid method for the determination of dimetridazole and ipronidazole in feeds is described. The compounds are extracted from a borate buffer (pH 8.65) with benzene, partitioned into IN HC1, and then partitioned back into benzene from a basic aqueous phase. The benzene extract is concentrated and injected onto a nonpolar (Apiezon L) gas chromatographic column for determination by 63Ni electroncapture detection. Recoveries from feeds of various composition, spiked at 0.2 ppm with both dimetridazole and ipronidazole, ranged from 70 to 115%; for the same feeds spiked at 1 ppm or more, the recoveries were greater than 80%. Carbadox, furazolidone, levamisole, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, arsanilic acid, piperazine, penicillin, and commonly added vitamins and minerals do not interfere. A 2-dimensional thin layer chromatographic system is presented as a means of additional identification.

Quantitation of Plasma Aldosterone by Radioimmunoassay

1972 ◽  
Vol 18 (11) ◽  
pp. 1395-1402 ◽  
Author(s):  
M J St Cyr ◽  
J M Sancho ◽  
J C Melby
Keyword(s):  
Thin Layer ◽  
Upright Position ◽  
Plasma Aldosterone ◽  
Chromatographic System ◽  
Normal Plasma ◽  
Accuracy And Precision

Abstract Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone-18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin-layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine subjects is 8.6 ± 3.8 ng/100 ml; after 2 h in the upright position, 18.3 ± 9.5 ng/100 ml. Eighteen months of experience with the method have proved it to be reliable with respect to reproducibility, accuracy, and precision.

scholarly journals Separation and Quantitation of Alkylphosphocholines and Analogues of Different Liposome Formulations by HPTLC

2001 ◽  
Vol 84 (4) ◽  
pp. 1277-1282 ◽  
Author(s):  
Hannelore Ratz ◽  
Horst Schnell ◽  
Matthias Rischer ◽  
Hans-Jörg Eibl
Keyword(s):  
Quality Control ◽  
Oral Administration ◽  
Thin Layer ◽  
High Performance ◽  
Degradation Products ◽  
Cytostatic Activity ◽  
Chromatographic System ◽  
Liposomal Formulations ◽  
Mobile Phases ◽  
Related Compounds

Abstract High-performance thin-layer chromatographic (HPTLC) analysis of non UV-active phospholipids in biological matrixes is a common method for separation, detection, and quantitation. Liposomes containing new alkylphosphocholines and analogues with enhanced cytostatic activity had been prepared. The liposomal formulations were designed to enable the intravenous application of the alkylphosphocholines and analogues and to reduce dose-limiting toxicities observed after oral administration. For quality control the liposomes were analyzed by HPTLC for content of 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), cholesterol, alkylphosphocholines, and analogues and their related compounds (main degradation products). Due to the differences in lipophily of the compounds, different mobile phases were necessary to achieve separation. Automated Multiple Development was used to reduce the number of plates and to improve the selectivity and the capacity of the chromatographic system to separate the described alkylphosphocholines and analogues from DPPG and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine in one chromatographic system.

Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.

1980 ◽  
Vol 26 (3) ◽  
pp. 403-405
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic System ◽  
Continuous Development ◽  
One Dimensional ◽  
Layer Chromatography

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.

Thin-layer chromatographic system for identification and quantitation of potato tuber glycoalkaloids

1978 ◽  
Vol 26 (6) ◽  
pp. 1453-1454 ◽  
Author(s):  
Larry S. Cadle ◽  
David A. Stelzig ◽  
Katherine L. Harper ◽  
Robert J. Young
Keyword(s):  
Thin Layer ◽  
Potato Tuber ◽  
Chromatographic System

scholarly journals Development of the methodology for the estimation of enalapril maleate in medicines

2017 ◽  
Author(s):  
L. S. Logoyda ◽  
D. B. Korobko
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Detection Limits ◽  
Chromatographic System ◽  
Eligibility Criteria ◽  
Chromatography Method ◽  
Enalapril Maleate ◽  
Number Of Components ◽  
Layer Chromatography

Introduction. Thin layer chromatography, or TLC, is a method for analyzing mixtures by separating the compounds in the mixture. TLC can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound.The aim of the study – to develop a thin layer chromatography method for the estimation enalapril in medicines.Research methods. The present study is assessed system solvents of enalapril maleate for thin layer chromatography.Results and Discussion. Method of identification of enalapril maleate in medicines by TLC was developed. We established that the most optimal Rf observed using mobile phase: ammonia (25 %) – propanol (30:70). The detection limits of enalapril maleate in this system are 0.2 mcg. However, those mobile phase is the most express. We explored the validation characteristics – specificity and suitability of the chromatographic system that met, the eligibility criteria established by the SPU.Conclusions. We developed chromatographic methods of identification of enalapril maleate in medicines. The proposed method is rapid, economical and simple.

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