Combined application of ion-exchange chromatographic methods for the study of “minor basic amino acids”

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.

Download Full-text

Displacement chromatography on synthetic ion-exchange resins. 5. Separation of the basic amino-acids

Biochemical Journal ◽

10.1042/bj0460334 ◽

1950 ◽

Vol 46 (3) ◽

pp. 334-340 ◽

Cited By ~ 32

Author(s):

S. M. Partridge ◽

R. C. Brimley ◽

K. W. Pepper

Keyword(s):

Amino Acids ◽

Ion Exchange ◽

Ion Exchange Resins ◽

Displacement Chromatography ◽

Basic Amino Acids ◽

Exchange Resins

Download Full-text

An ion-exchange chromatography procedure for the isolation and concentration of basic amino acids and polyamines from complex biological samples prior to high-performance liquid chromatography

Analytical Biochemistry ◽

10.1016/0003-2697(91)90273-v ◽

1991 ◽

Vol 199 (1) ◽

pp. 86-92 ◽

Cited By ~ 28

Author(s):

Rebecca S. Gilbert ◽

Guadelupe G. Gonzalez ◽

Leo Hawel ◽

Craig V. Byus

Keyword(s):

Amino Acids ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Ion Exchange ◽

Biological Samples ◽

High Performance ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Basic Amino Acids

Download Full-text

ENDOGENOUS RENAL CLEARANCE RATES OF FREE AMINO ACIDS IN PRE-PUBERTAL CHILDREN (Employing an Accelerated Procedure for Elution Chromatography of Basic Amino Acids on Ion Exchange Resin)

PEDIATRICS ◽

10.1542/peds.36.4.592 ◽

1965 ◽

Vol 36 (4) ◽

pp. 592-598 ◽

Cited By ~ 1

Author(s):

Charles R. Scriver ◽

Eluned Davies

Keyword(s):

Amino Acids ◽

Ion Exchange ◽

Renal Clearance ◽

Individual Variation ◽

Free Amino Acids ◽

Free Amino ◽

Plasma Concentrations ◽

Exchange Chromatography ◽

Clearance Rates ◽

Basic Amino Acids

Six male and three female children in good health and between the ages of 3 and 10 years were studied. Fasting plasma concentrations and urine excretion rates of free amino acids were measured using automated ion exchange chromatography, utilizing a modified method for analysis of the basic amino acids. Endogenous clearance rates and net tubular absorption of plasma amino acids were also calculated. The plasma concentration of free amino acids is generally slightly lower than in the adult subjects and renal clearance rates are slightly higher. Less than 5% of the filtered amino acid load is excreted in the urine. Intra-individual variation tends to be smaller than inter-individual variation.

Download Full-text