Determination of steroids by paper strip elution chromatography

1961 ◽  
Vol 5 ◽  
pp. 332-335 ◽  
Author(s):  
D.M. Abelson ◽  
Philip K. Bondy ◽  
J. Piskorski
Keyword(s):  
Paper Strip ◽  
Elution Chromatography

scholarly journals Simultaneous determination of mycophenolic acid and its glucuronide in human plasma using a simple high-performance liquid chromatography procedure

1998 ◽  
Vol 44 (7) ◽  
pp. 1481-1488 ◽  
Author(s):  
Maria Shipkova ◽  
Paul Dieter Niedmann ◽  
Victor William Armstrong ◽  
Ekkehard Schütz ◽  
Eberhard Wieland ◽  
...  
Keyword(s):  
Mycophenolic Acid ◽  
High Performance ◽  
Internal Standard ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Hplc Method ◽  
Free Concentration ◽  
Elution Chromatography ◽  
Glucuronide Metabolite

Abstract We describe a reversed-phase HPLC method for determination of total mycophenolic acid (MPA), its free concentration (MPAf), and the glucuronide metabolite (MPAG), based on simple sample preparation and gradient elution chromatography. The compounds were quantified in parallel by absorbance at 254 nm and 215 nm in the internal standard mode. Linearity was verified up to 50 mg/L for MPA and up to 500 mg/L for MPAG (r >0.999). Detection limits at 215 and 254 nm were, respectively, 0.01 and 0.03 mg/L for MPA, and 0.03 and 0.1 mg/L for MPAG. The recovery of MPA was 95–106%;recovery of MPAG was 96–106%. The imprecision (CV) for MPA (0.2–25 mg/L) was <8.4% (254 nm) and <4.4% (215 nm) within day (n = 12) and <9.2% (254 nm) and <6.2% (215 nm) between days (n = 12). The imprecision for MPAG (10–250 mg/L) was <4.9% (254 nm) and <3.4% (215 nm) within day, and <6.1% (254 nm) and <5.9% (215 nm) between days. For quantification of MPAf, 100 μL of ultrafiltrate was applied directly to the column. The detection limit was 0.005 mg/L at 215 nm and 0.015 mg/L at 254 nm. In the range between 18–210 μg/L, the within-day CVs were <11.8% (n = 12) and the between-day CVs were <15.8% (n = 12).

A MICROMETHOD FOR THE DETERMINATION OF SUGARS

1950 ◽  
Vol 28b (11) ◽  
pp. 676-682 ◽  
Author(s):  
J. E. Stone ◽  
M. J. Blundell
Keyword(s):  
Phosphoric Acid ◽  
Steam Distillation ◽  
Mean Deviation ◽  
Paper Strip ◽  
The Mean ◽  
Time Required

Certain pentoses and hexoses were determined by measuring spectrophotometrically the yields of furfural and hydroxymethylfurfural obtained on steam distilling with syrupy phosphoric acid. The yields, based on the theoretical recovery, were increased by increasing the temperature and the rate of steam distillation, and by decreasing the amount of sugar. At a steam rate producing 10 ml. per min. of distillate, at 195 °C. externally applied temperature, and with 2 mgm. sugar, the yields of aldehyde were from xylose 91.0%, ribose 74.9%, arabinose 60.6%, rhamnose 55.9%, galactose 20.0%, and glucose 15.5%. As little as 0.1 mgm. of each could be determined in this way, the mean deviation being usually less than ± 3% and the time required less than 10 min. Mixtures of sugars have been separated on a paper strip chromatogram and the individuals determined, without separate extraction, by steam distilling into phosphoric acid.

Paper Strip-based Fluorometric Determination of Cyanide with an Internal Reference

2016 ◽  
Vol 37 (8) ◽  
pp. 1320-1325 ◽  
Author(s):  
Dong-Nam Lee ◽  
Hyejin Seo ◽  
Ik-Soo Shin ◽  
Jong-In Hong
Keyword(s):  
Paper Strip ◽  
Fluorometric Determination ◽  
Internal Reference
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