Purification of quinoline yellow components using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase

2003 ◽  
Vol 989 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Hisao Oka ◽  
Ken-Ichi Harada ◽  
Masanao Suzuki ◽  
Kiyonaga Fujii ◽  
Masato Iwaya ◽  
...  
Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 91 ◽  
Author(s):  
Pian Zhang ◽  
Kang-Ling Zhu ◽  
Jun Zhang ◽  
Yan Li ◽  
Heng Zhang ◽  
...  

In order to obtain high-purity flavonoid products, the extracts from mulberry leaves were separated and purified via high-speed counter-current chromatography (HSCCC). Moreover, the product was detected via high-performance liquid chromatography (HPLC). The characteristic absorption wavelength of the rutin standard for HSCCC detection and HPLC analysis at 257 nm was tested by ultraviolet scanning analysis. The effect of solvent systems and mobile phase flow rate on the separation efficiency were then researched. Finally, the solvent system of V(ethyl acetate):V(n-butanol):V(water) = 4:1:5 was selected as the operating system for HSCCC. This work theoretically analyzed the impact of the molecular structure and polarity of flavonoids on the choice of solvent systems. The results showed that the mobile phase flow rate had a great influence on the separation efficiency. Furthermore, the separation efficiency increased as the mobile phase flow rate decreased. When the mobile phase flow rate was 5 mL/min, the peak time for flavonoids was 140 min, the retention of the stationary phase was 56.4%, and the purity of the product reached 93.8%. The results of this study greatly improved the purity of flavonoids in mulberry leaf and provided a strong support for the separation and purification of mulberry leaf extract.


Author(s):  
Jee Loong Hee ◽  
R. Santhosh ◽  
Kathy Simmons ◽  
Graham Johnson ◽  
David Hann ◽  
...  

In a civil aero-engine transmission system a number of bearings are used for shaft location and load support. A bespoke experimental test facility in the University of Nottingham’s Gas Turbine and Transmissions Research Centre (G2TRC) was created to investigate oil shedding from a location bearing. An engine representative ball bearing was installed in the rig and under-race lubrication was supplied via under-race feed to three locations under the inner race and cage. The oil was supplied in an engine representative manner but the delivery system was modified to provide circumferentially even flow. An electromagnetic load system was designed and implemented to allow engine representative axial loads between 5 and 35 kN to be applied to the bearing. In this phase of testing the rig was operated at shaft speeds between 1,000 rpm and 7,000 rpm for a range of oil flow rates and low and high load conditions. The rig was designed with good visual access and high speed imaging was used to investigate film formation and movement on surfaces close to the bearing. This paper presents images and qualitative observations of thin film formed on the static surfaces forming the outer-periphery of the bearing compartment as well as the gap between orbiting cage and static outer race. Quantitative film thickness was obtained at two circumferential locations (90° and 270° from top dead centre) and three axial locations, through sophisticated analysis of the high speed images. The effect on film thickness of the varied parameters rotational speed, axial load and oil feed input flow rate are presented in this paper. It was observed that for all axial planes of measurement in both co-current and counter-current regions film thickness decreases with increase in shaft rotational speed. At 5,000 and 7,000 rpm film thicknesses are around 0.75 mm – 1 mm and are similar at 90° and 270°; at 3,000 rpm films tend to be somewhat thicker at around 1.5 mm – 2 mm and are thicker in the counter current region, particularly closer to the bearing. It is suggested that at higher shaft speeds interfacial shear dominates whereas at lower speed the effect of gravity in slowing the film in the counter-current region causes a measureable difference. It was further observed that increasing the input oil flow rate from 5.2 litres per minute to 7.3 litres per minute did not produce significant effect on film thickness. However, the increase of axial bearing load from 10 kN to 30 kN yielded thicker films at the location above the cage. In all cases there was waviness on the film surface at the bearing outer periphery; imaging was not sufficient to see if the film surface close to the bearing is wavy.


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