scholarly journals Apolipoproteins, membrane cholesterol domains, and the regulation of cholesterol efflux.

1992 ◽  
Vol 33 (8) ◽  
pp. 1091-1097
Author(s):  
GH Rothblat ◽  
FH Mahlberg ◽  
WJ Johnson ◽  
MC Phillips
Keyword(s):  
Cholesterol Efflux ◽  
Membrane Cholesterol

scholarly journals Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Reproduction ◽  
2015 ◽  
Vol 149 (1) ◽  
pp. 87-99 ◽  
Author(s):  
B Macías-García ◽  
L González-Fernández ◽  
S C Loux ◽  
A M Rocha ◽  
T Guimarães ◽  
...  
Keyword(s):  
Cholesterol Efflux ◽  
Cholesterol Oxidation ◽  
Membrane Cholesterol ◽  
Ros Production ◽  
Oxygen Species ◽  
Factors Affecting ◽  
Sperm Binding ◽  
Cholesterol Levels ◽  
Calcium Bicarbonate ◽  
Fertilization Capacity

Repeatable methods for IVF have not been established in the horse, reflecting the failure of standard capacitating media to induce changes required for fertilization capacity in equine sperm. One important step in capacitation is membrane cholesterol efflux, which in other species is triggered by cholesterol oxidation and is typically enhanced using albumin as a sterol acceptor. We incubated equine sperm in the presence of calcium, BSA, and bicarbonate, alone or in combination. Bicarbonate induced an increase in reactive oxygen species (ROS) that was abolished by the addition of calcium or BSA. Bicarbonate induced protein tyrosine phosphorylation (PY), even in the presence of calcium or BSA. Incubation at high pH enhanced PY but did not increase ROS production. Notably, no combination of these factors was associated with significant cholesterol efflux, as assessed by fluorescent quantitative cholesterol assay and confirmed by filipin staining. By contrast, sperm treated with methyl-β-cyclodextrin showed a significant reduction in cholesterol levels, but no significant increase in PY or ROS. Presence of BSA increased sperm binding to bovine zonae pellucidae in all three stallions. These results show that presence of serum albumin is not associated with a reduction in membrane cholesterol levels in equine sperm, highlighting the failure of equine sperm to exhibit core capacitation-related changes in a standard capacitating medium. These data indicate an atypical relationship among cholesterol efflux, ROS production, and PY in equine sperm. Our findings may help to elucidate factors affecting failure of equine IVF under standard conditions.

scholarly journals Membrane Cholesterol Efflux Drives Tumor-Associated Macrophage Reprogramming and Tumor Progression

2019 ◽  
Vol 29 (6) ◽  
pp. 1376-1389.e4 ◽  
Author(s):  
Pieter Goossens ◽  
Juan Rodriguez-Vita ◽  
Anders Etzerodt ◽  
Marion Masse ◽  
Olivia Rastoin ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Cholesterol Efflux ◽  
Membrane Cholesterol ◽  
Tumor Associated Macrophage

ω-3 Fatty acids in smooth muscle cell phospholipids increase membrane cholesterol efflux

Lipids ◽  
1995 ◽  
Vol 30 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Eric Dusserre ◽  
Thérèse Pulcini ◽  
Marie-Claude Bourdillon ◽  
Maryvonne Ciavatti ◽  
François Berthezene
Keyword(s):  
Fatty Acids ◽  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cholesterol Efflux ◽  
Membrane Cholesterol

scholarly journals Double Potential Pulse Chronocoulometry for Detection of Plasma Membrane Cholesterol Efflux at Disk Platinum Microelectrodes

2014 ◽  
Vol 161 (6) ◽  
pp. B111-B116 ◽  
Author(s):  
Richard H. West ◽  
Hui Lu ◽  
Kendrick Shaw ◽  
Hillel J. Chiel ◽  
Thomas J. Kelley ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cholesterol Efflux ◽  
Membrane Cholesterol ◽  
Plasma Membrane Cholesterol

The Anti-Atherogenic Properties of Sesamin are Mediated via Improved Macrophage Cholesterol Efflux Through PPARγ1-LXRα and MAPK Signaling

2014 ◽  
Vol 84 (1-2) ◽  
pp. 79-91 ◽  
Author(s):  
Amin F. Majdalawieh ◽  
Hyo-Sung Ro
Keyword(s):  
Cholesterol Efflux ◽  
Transcriptional Activity ◽  
Molecular Mechanisms ◽  
Foam Cell ◽  
Mapk Signaling ◽  
Luciferase Reporter ◽  
Foam Cell Formation ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Macrophage Cholesterol Efflux

Background: Foam cell formation resulting from disrupted macrophage cholesterol efflux, which is triggered by PPARγ1 and LXRα, is a hallmark of atherosclerosis. Sesamin and sesame oil exert anti-atherogenic effects in vivo. However, the exact molecular mechanisms underlying such effects are not fully understood. Aim: This study examines the potential effects of sesamin (0, 25, 50, 75, 100 μM) on PPARγ1 and LXRα expression and transcriptional activity as well as macrophage cholesterol efflux. Methods: PPARγ1 and LXRα expression and transcriptional activity are assessed by luciferase reporter assays. Macrophage cholesterol efflux is evaluated by ApoAI-specific cholesterol efflux assays. Results: The 50 μM, 75 μM, and 100 μM concentrations of sesamin up-regulated the expression of PPARγ1 (p< 0.001, p < 0.001, p < 0.001, respectively) and LXRα (p = 0.002, p < 0.001, p < 0.001, respectively) in a concentration-dependent manner. Moreover, 75 μM and 100 μM concentrations of sesamin led to 5.2-fold (p < 0.001) and 6.0-fold (p<0.001) increases in PPAR transcriptional activity and 3.9-fold (p< 0.001) and 4.2-fold (p < 0.001) increases in LXR transcriptional activity, respectively, in a concentration- and time-dependent manner via MAPK signaling. Consistently, 50 μM, 75 μM, and 100 μM concentrations of sesamin improved macrophage cholesterol efflux by 2.7-fold (p < 0.001), 4.2-fold (p < 0.001), and 4.2-fold (p < 0.001), respectively, via MAPK signaling. Conclusion: Our findings shed light on the molecular mechanism(s) underlying sesamin’s anti-atherogenic effects, which seem to be due, at least in part, to its ability to up-regulate PPARγ1 and LXRα expression and transcriptional activity, improving macrophage cholesterol efflux. We anticipate that sesamin may be used as a therapeutic agent for treating atherosclerosis.

Sign in / Sign up

Export Citation Format

Share Document