scholarly journals Amino acid sequence of the 26 kDa subunit of legumin-type seed storage protein of common buckwheat (Fagopyrum esculentum Moench): molecular characterization and phylogenetic analysis

2003 ◽  
Vol 63 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Sangeeta Bharali ◽  
Nikhil K. Chrungoo
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Molecular Characterization ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Fagopyrum Esculentum ◽  
Common Buckwheat ◽  
Fagopyrum Esculentum Moench

scholarly journals Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench

2004 ◽  
Vol 56 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Mira Milisavljevic ◽  
Miroslav Konstantinovic ◽  
Jelena Brkljacic ◽  
Vesna Maksimovic
Keyword(s):  
Storage Protein ◽  
Protein Gene ◽  
Seed Storage Protein ◽  
Regulatory Region ◽  
Seed Storage ◽  
Regulatory Elements ◽  
Computer Prediction ◽  
Storage Protein Gene ◽  
Seed Storage Protein Gene ◽  
Fagopyrum Esculentum Moench

Using the modified 5?-RACE approach, a fragment containing the 955 bp long 5?- regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory elements possibly involved in tissue specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research, but also as a useful tool for biotechnological application.

scholarly journals Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)

2010 ◽  
Vol 62 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Gordana Timotijevic ◽  
Mira Milisavljevic ◽  
Svetlana Radovic ◽  
M.M. Konstantinovic ◽  
Vesna Maksimovic
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cdna Library ◽  
Seed Development ◽  
Potential Role ◽  
Aspartic Proteinase ◽  
Fagopyrum Esculentum ◽  
High Homology ◽  
Aspartic Proteinases ◽  
Fagopyrum Esculentum Moench

Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.

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