γ-Linolenic acid purification from seed oil sources by argentated silica gel chromatography column

2000 ◽  
Vol 36 (4) ◽  
pp. 341-354 ◽  
Author(s):  
J.L Guil-Guerrero ◽  
P Campra-Madrid ◽  
El-Hassan Belarbi
Keyword(s):  
Linolenic Acid ◽  
Silica Gel ◽  
Seed Oil ◽  
Gel Chromatography ◽  
Chromatography Column ◽  
Silica Gel Chromatography

Purification of α-Linolenic Acid from Flaxseed Oil by Silver-Silica Gel Chromatography Column

2012 ◽  
Vol 610-613 ◽  
pp. 3580-3586
Author(s):  
Jian Xia Guo ◽  
Chang Lu Wang ◽  
Zhi Jian Wu
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Silica Gel ◽  
Flaxseed Oil ◽  
Ethyl Esters ◽  
Gel Chromatography ◽  
Distillation Method ◽  
Chromatography Column ◽  
Silica Gel Chromatography

Flaxseed oil contains significant amounts of essential fatty acid, α-linolenic acid (ALA) with a content of 61% of the total fatty acid. ALA is attracting increasing attention because of their importance to human health. ALA has various physiological activities whose absence in the diet is responsible for the development of a wide variety of abnormalities. Highly purified ALA is required on pharmaceutical applications. This work reports as recovery of highly purified ALA from flaxseed oil by means of a process, which involves simultaneous oil saponification–extraction, followed by the ethyl esterification of fatty acids. Thereafter, the polyunsaturated fatty acids (PUFA) were concentrated by molecular distillation method, and ethyl esters below ALA were fractionated from ethyl–PUFA concentrate by mean of open column chromatography with silver–silica gel as stationary phase. The recovery in the combined process was 79%, and the final purity was 94.7%. Therefore, highly pure PUFA ALA could be procured by argentated silica gel chromatography column.

scholarly journals CAAC Boranes. Synthesis and characterization of cyclic (alkyl) (amino) carbene borane complexes from BF3 and BH3

2010 ◽  
Vol 6 ◽  
pp. 709-712 ◽  
Author(s):  
Julien Monot ◽  
Louis Fensterbank ◽  
Max Malacria ◽  
Emmanuel Lacôte ◽  
Steven J Geib ◽  
...  
Keyword(s):  
Silica Gel ◽  
Synthesis And Characterization ◽  
Gel Chromatography ◽  
Nmr Analysis ◽  
X Ray ◽  
X Ray Crystallography ◽  
In Situ Formation ◽  
Silica Gel Chromatography

In situ formation of two cyclic (alkyl) (amino) carbenes (CAACs) followed by addition of BF3•Et2O provided the first two examples of CAAC–BF3 complexes: 1-(2,6-diisopropylphenyl)-3,5,5-trimethyl-3-phenylpyrrolidin-2-ylidene trifluoroborane, and 2-(2,6-diisopropylphenyl)-3,3-dimethyl-2-azaspiro[4.5]decan-1-ylidene trifluoroborane. These CAAC–BF3 complexes are robust compounds that are stable to ambient laboratory conditions and silica gel chromatography. They were characterized by spectroscopy and X-ray crystallography. In contrast, a CAAC complex with borane (BH3) was readily formed in situ according to 1H and 11B NMR analysis, but did not survive the workup conditions. These results set the stage for further studies of the chemistry of CAAC boranes.

scholarly journals Determination of Phospholipid Fractions in Red Blood Cells in Retarded Physically Handicapped Children

1973 ◽  
Vol 10 (1-6) ◽  
pp. 23-27 ◽  
Author(s):  
Fay M. John ◽  
G. C. Forrest
Keyword(s):  
Red Blood Cells ◽  
Silica Gel ◽  
Blood Cells ◽  
Red Cells ◽  
Gel Chromatography ◽  
Handicapped Children ◽  
Silica Gel Chromatography ◽  
Physically Handicapped

Phospholipids from the red cells of 85 children, both normal and belonging to various clinical groups, have been extracted and subjected to silica gel chromatography. The several phospholipids have been quantitated. The results have not proved to be useful in diagnosis and hence do not confirm earlier reports. The problems associated with analysis of phospholipids by this type of method are discussed.

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