Computer coupled substrate feeding strategies for efficient conversion of d-sorbitol to l-sorbose in fed-batch culture

2001 ◽  
Vol 36 (8-9) ◽  
pp. 829-834 ◽  
Author(s):  
R Giridhar ◽  
Ashok K Srivastava
Keyword(s):  
Batch Culture ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Efficient Conversion ◽  
Substrate Feeding ◽  
Fed Batch Culture

scholarly journals Alpha-Ketoglutaric Acid Production from a Mixture of Glycerol and Rapeseed Oil by Yarrowia lipolytica Using Different Substrate Feeding Strategies

2020 ◽  
Vol 12 (15) ◽  
pp. 6109 ◽  
Author(s):  
Anita Rywińska ◽  
Ludwika Tomaszewska-Hetman ◽  
Magdalena Rakicka-Pustułka ◽  
Piotr Juszczyk ◽  
Waldemar Rymowicz
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Culture ◽  
Rapeseed Oil ◽  
Feeding Strategy ◽  
Wild Strain ◽  
Control Culture ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Repeated Batch ◽  
Substrate Feeding

The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention of many scientists as an alternative to its common chemical synthesis. The present study aimed to evaluate the effect of the feeding strategy of substrates, i.e., glycerol (G = 20 g·dm−3) and rapeseed oil (O = 20 g·dm−3), on yeast growth and the parameters of KGA biosynthesis by a wild strain Yarrowia lipolytica A-8 in fed-batch and repeated-batch cultures. The effectiveness of KGA biosynthesis was demonstrated to depend on thiamine concentration and the substrate feeding method. In the fed-batch culture incubated with 3 µg·dm−3 of thiamine and a substrate feeding variant 2G(_OGO), KGA was produced in the amount of 62.1 g·dm−3 at the volumetric production rate of 0.37 g·dm−3·h−1. These values of KGA production parameters were higher than these obtained in the control culture (with rapeseed oil only). During 10 cycles of the 1788-h repeated-batch culture carried out acc. to the feeding strategy 2G(_OGO), in the last 5 cycles the yeast produced from 55.6 to 58.2 g·dm−3 of KGA and maximally 2.9 g·dm−3 of the pyruvic acid as a by-product.

scholarly journals Application of different feeding strategies in fed batch culture for pullulanase production using sago starch

2014 ◽  
Vol 102 ◽  
pp. 962-969 ◽  
Author(s):  
Shankar R. ◽  
Madihah M.S. ◽  
Shaza E.M. ◽  
Nur Aswati K.O. ◽  
Suraini A.A. ◽  
...  
Keyword(s):  
Batch Culture ◽  
Feeding Strategies ◽  
Sago Starch ◽  
Fed Batch ◽  
Fed Batch Culture

Maximum production in a bakers' yeast fed-batch culture by a tubing method

1981 ◽  
Vol 23 (9) ◽  
pp. 2069-2081 ◽  
Author(s):  
Kazuo Dairaku ◽  
Yoshihiko Yamasaki ◽  
Kazuo Kuki ◽  
Suteaki Shioya ◽  
Takeichiro Takamatsu
Keyword(s):  
Batch Culture ◽  
Fed Batch ◽  
Maximum Production ◽  
Fed Batch Culture
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