scholarly journals Alpha-Ketoglutaric Acid Production from a Mixture of Glycerol and Rapeseed Oil by Yarrowia lipolytica Using Different Substrate Feeding Strategies

2020 ◽  
Vol 12 (15) ◽  
pp. 6109 ◽  
Author(s):  
Anita Rywińska ◽  
Ludwika Tomaszewska-Hetman ◽  
Magdalena Rakicka-Pustułka ◽  
Piotr Juszczyk ◽  
Waldemar Rymowicz
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Culture ◽  
Rapeseed Oil ◽  
Feeding Strategy ◽  
Wild Strain ◽  
Control Culture ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Repeated Batch ◽  
Substrate Feeding

The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention of many scientists as an alternative to its common chemical synthesis. The present study aimed to evaluate the effect of the feeding strategy of substrates, i.e., glycerol (G = 20 g·dm−3) and rapeseed oil (O = 20 g·dm−3), on yeast growth and the parameters of KGA biosynthesis by a wild strain Yarrowia lipolytica A-8 in fed-batch and repeated-batch cultures. The effectiveness of KGA biosynthesis was demonstrated to depend on thiamine concentration and the substrate feeding method. In the fed-batch culture incubated with 3 µg·dm−3 of thiamine and a substrate feeding variant 2G(_OGO), KGA was produced in the amount of 62.1 g·dm−3 at the volumetric production rate of 0.37 g·dm−3·h−1. These values of KGA production parameters were higher than these obtained in the control culture (with rapeseed oil only). During 10 cycles of the 1788-h repeated-batch culture carried out acc. to the feeding strategy 2G(_OGO), in the last 5 cycles the yeast produced from 55.6 to 58.2 g·dm−3 of KGA and maximally 2.9 g·dm−3 of the pyruvic acid as a by-product.

Biosynthesis of keto acids by fed-batch culture of Yarrowia lipolytica WSH-Z06

2017 ◽  
Vol 243 ◽  
pp. 1037-1043 ◽  
Author(s):  
Weizhu Zeng ◽  
Hailin Zhang ◽  
Sha Xu ◽  
Fang Fang ◽  
Jingwen Zhou
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Culture ◽  
Fed Batch ◽  
Keto Acids ◽  
Fed Batch Culture

scholarly journals Towards the development of automated fed-batch cell culture processes at microscale

BioTechniques ◽  
2019 ◽  
Vol 67 (5) ◽  
pp. 238-241
Author(s):  
Vincent Wiegmann ◽  
Maria Giaka ◽  
Cristina Bernal Martinez ◽  
Frank Baganz
Keyword(s):  
Feeding Strategy ◽  
Viable Cell ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Cho Cell ◽  
Feed Medium ◽  
Cell Densities ◽  
The Impact ◽  
Batch Cell ◽  
Selection Of

Aim: To investigate the impact of various feeding strategies on the growth and productivity of a GS-CHO cell line. Methods: Feed additions were conducted at fixed volumes or linked to a marker such as cell growth or metabolism and added as bolus or near-continuously using the automated feeding module of the micro-Matrix (Applikon). Results: The selected feeding regimens supported maximum viable cell densities of up to 1.9 × 107 cells ml−1 and final titers of up to 1.13 g l−1. Differences in growth and titer between feeding strategies were insignificant, with the exception of one feeding strategy. Conclusion: As the more complex feeding strategies did not create an advantage, the selection of a simple feeding strategy such as bolus or continuous addition of feed medium is preferred.

Effects of feeding strategies on the performance of an anaerobic discontinuous reactor containing immobilized biomass with circulation system for liquid-phase mixing

2004 ◽  
Vol 49 (11-12) ◽  
pp. 303-310 ◽  
Author(s):  
A.A. Orra ◽  
S.M. Ratusznei ◽  
J.A.D. Rodrigues ◽  
E. Foresti ◽  
M. Zaiat
Keyword(s):  
Liquid Phase ◽  
High Efficiency ◽  
Feeding Strategy ◽  
Current System ◽  
Operating Conditions ◽  
Circulation System ◽  
Feeding Strategies ◽  
Order Kinetic ◽  
Fed Batch ◽  
Batch Mode

Data on the influence of feeding strategy on the performance of a fed-batch anaerobic sequencing reactor containing biomass immobilized on polyurethane foam and subjected to liquid phase circulation are presented and discussed. Six-hour cycles, temperature of 30°C and circulation flow rate of 6 L/h were used. During each cycle 890 mL of synthetic domestic wastewater, with organic matter concentration of 500 mgCOD/L were fed to the reactor. The feeding strategies were implemented using fill times of 6 min (batch mode), 60, 120, 240 (fed-batch/batch mode) and 360 min (fed-batch mode). The system attained high efficiency and stability for all the operating conditions, and the substrate removal efficiency based on filtered samples presented a slight decrease from 85% to 81% when fill time was increased from 6 min to 360 min. A model considering a first-order kinetic equation was fitted to the experimental data. The apparent kinetic parameters for both batch and fed-batch phases were estimated, thus permitting evaluation of the influence of the feeding strategy on the reactor performance. The current system may be considered flexible in terms of the operating conditions it is subjected to.

Automatic Control Design for Optimal Strategy in Fed-batch Culture with Coupled Feed of Glycerol and Alkali

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
Shan Jiang ◽  
Jianxiong Ye ◽  
Enmin Feng ◽  
Zhilong Xiu
Keyword(s):  
Control System ◽  
Automatic Control ◽  
Batch Culture ◽  
Feeding Strategy ◽  
Nonlinear Dynamical System ◽  
Control Design ◽  
Fed Batch ◽  
Multi Stage ◽  
Fed Batch Culture ◽  
Monitored Control System

In this paper, we study the production of 1,3-propanediol in fed-batch culture with coupled feed of glycerol and alkali. On the basis of the numerical solution to an optimal control problem subjected to a multi-stage nonlinear dynamical system, we propose a realizable optimal feeding strategy. Correspondingly, an automatic control system is designed for the fermenter, which involves the designs of control circuit, software flow control and computer monitored control system. This contribution successfully achieves a goal of combining a mathematically theoretical analysis with an automatic control design in the laboratory.

scholarly journals Production of L (+) Lactic Acid by Lactobacillus casei Ke11: Fed Batch Fermentation Strategies

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 151
Author(s):  
Paola Monteiro de Oliveira ◽  
Larissa Provasi Santos ◽  
Luciana Fontes Coelho ◽  
Paulo Marcelo Avila Neto ◽  
Daiane Cristina Sass ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Batch Fermentation ◽  
Lactobacillus Casei ◽  
Culture Media ◽  
Feeding Strategy ◽  
High Yield ◽  
Feeding Strategies ◽  
Efficient Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Lactic acid and its derivatives are widely used in pharmaceutical, leather, textile and food industries. However, until now there have been few systematic reports on fed-batch fermentation for efficient production and high concentration of l-lactic acid by lactic acid bacteria. This study describes the obtainment of L (+) lactic acid from sucrose using the Lactobacillus casei Ke11 strain through different feeding strategies using an accessible pH neutralizer such as CaCO3. The exponential feeding strategy can increase lactic acid production and productivity (175.84 g/L and 3.74 g/L/h, respectively) with a 95% yield, avoiding inhibition by high initial substrate concentration and, combined with the selected agent controller, avoids the cellular stress that could be caused by the high osmotic pressure of the culture media. The purification of the acid using charcoal and celite, followed by the use of a cation exchange column proved to be highly efficient, allowing a high yield of lactic acid, high removal of sugars and proteins. The described process shows great potential for the production of lactic acid, as well as the simple, efficient and low-cost purification method. This way, this work is useful to the large-scale fermentation of L. casei Ke11 for production of l-lactic acid.

Substrate Feeding Strategy Integrated with a Biomass Bayesian Estimator for a Biotechnological Process

2016 ◽  
Vol 14 (6) ◽  
pp. 1187-1200 ◽  
Author(s):  
Adriana Amicarelli ◽  
Lucía Quintero Montoya ◽  
Fernando di Sciascio
Keyword(s):  
Nonlinear Filtering ◽  
Feeding Strategy ◽  
Bayesian Estimator ◽  
Biomass Estimation ◽  
Fed Batch ◽  
Product Concentration ◽  
Batch Cultures ◽  
Strongly Nonlinear ◽  
Substrate Feeding ◽  
Filtering Techniques

Abstract This work proposes a substrate feeding strategy for a bioprocess integrated with a biomass estimator based in nonlinear filtering techniques. The performance of the proposed estimator and the substrate strategy are illustrated for the δ-endotoxin production of Bacillus thuringiensis (Bt) in batch and fed batch cultures. Nonlinear filtering techniques constitutes an adequate option as estimation tool because of the strongly nonlinear dynamics of this bioprocess and also due to nature of the uncertainties and perturbations that cannot be supposed Gaussians distributed. Biomass estimation is performed from substrate and dissolved oxygen. Substrate feeding strategy is intended to obtain high product concentration. Simulations results along with their experimental verifications demonstrate the acceptable performance of the proposed biomass estimator and the substrate feeding strategy.

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