Effects of long-term, light exercise under restricted feeding on age-related changes in physiological and metabolic variables in male Wistar rats

The in vitro renal cortical slice model was used to study: 1) the effects on the kidney of some haloalkanes and haloalkenes using 3-month-old male Wistar rats; 2) influence of age and sex on renal cortical slice indices in non-treated rats; and 3) effects of 1,2-dichloropropane on the slices after pretreatment of 3-month-old male Wistar rats with DL-butathionine-[S,R]-sulphoximine. The most nephrotoxic chemical used was 1,3-dichloropropene, which caused a total depletion in the levels of reduced glutathione, a high peroxidation of lipid (about three thousand-fold with respect to control), a significant release of tubular enzymes into the medium, and loss of organic anion ( p-aminohippurate) accumulation. All the chemicals affected the cytosol more than the brush border. The most remarkable age-related differences in the untreated slices were the progressive decrease of reduced glutathione (p<0.05 from three months of age), and an increase in lactate dehydrogenase release into the medium (p<0.05 from six months of age). By contrast, sex differences were slight. The ‘treatment with 1,2-dichloropropane of slices prepared from rats pretreated with DL-butathionine-[S,R]-sulphoximine significantly increased the depletion of glutathione content (p<0.05) and malondialdehyde release in the medium (p<0.001) caused by the solvent alone.

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Glucose Utilization in Rat Hippocampus after Long-Term Recovery from Ischemia

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/jcbfm.1990.96 ◽

1990 ◽

Vol 10 (4) ◽

pp. 542-549 ◽

Cited By ~ 19

Author(s):

Thomas Beck ◽

Andreas Wree ◽

Axel Schleicher

Keyword(s):

Wistar Rats ◽

Carotid Arteries ◽

Glucose Utilization ◽

Pyramidal Cells ◽

Cresyl Violet ◽

Forebrain Ischemia ◽

Ischemic Insult ◽

Histological Damage ◽

Male Wistar Rats

The influence on hippocampal glucose utilization of a transient 10-min forebrain ischemia was quantified in male Wistar rats after 2 and 3 weeks as well as after 3 months by application of the [14C]2-deoxyglucose technique. Ischemia was induced by occlusion of the carotid arteries and simultaneous lowering of the blood pressure to 40 mm Hg. For identification of the hippocampal architecture, sections were stained for perikarya (cresyl violet) and for acetylcholinesterase. The hippocampal regions clearly showed different responses to the ischemic insult. The necrotic pyramidal cells being almost completely removed, significant increases in glucose utilization occurred in most layers of the CA1 sector at 2 and 3 weeks post ischemia, while widespread reductions prevailed in all other sectors and the dentate gyrus. At 3 months after the ischemic insult, glucose utilization was reduced in all hippocampal structures including the CA1 region. The increases in glucose utilization in the CA1 sector are suggested to indicate long-lasting presynaptic hyperexcitation, while the widespread reductions in glucose utilization demonstrate that neuronal activity is also altered in hippocampal areas that do not show major histological damage.

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Age-related changes in activity of cerebellum Purkinje cells, shape of the complex spike, and locomotion of Wistar rats under effect of ethanol

Journal of Evolutionary Biochemistry and Physiology ◽

10.1134/s0022093012040056 ◽

2012 ◽

Vol 48 (4) ◽

pp. 414-421

Author(s):

T. V. Karelina

Keyword(s):

Purkinje Cells ◽

Wistar Rats ◽

Complex Spike ◽

Age Related ◽

Age Related Changes

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Effects of Long-Term Administration of Lithium and Hydrochlorothiazide in Rats

Metal-Based Drugs ◽

10.1155/mbd.1999.87 ◽

1999 ◽

Vol 6 (2) ◽

pp. 87-93 ◽

Cited By ~ 6

Author(s):

Felicia Loghin ◽

Adriana Olinic ◽

Daniela-Saveta Popa ◽

Carmen Socaciu ◽

Sorin E. Leucuta

Keyword(s):

Wistar Rats ◽

Histopathological Examination ◽

Concomitant Administration ◽

Histological Changes ◽

Association Group ◽

Term Administration ◽

Male Wistar Rats ◽

Kidney Liver ◽

Lithium Lactate

The biochemical and histological changes following 60 days administration of daily doses equivalent to 1/20 LD50 of lithium lactate and hydrochlorothiazide, as such and in association, were studied in male Wistar rats. No mortality or overt signs of toxicity were observed during the experiment and the serum activities of transaminases, alkaline phosphatase and cholinesterase were not significantly modified compared to controls. The histopathological examination of all the investigated organs: kidney, liver, brain and spleen, revealed significant lesions which were time-dependant and more pronounced in the association group. Although the changes were mostly inflammatory and conqestive, it was proved that the concomitant administration of lithium and hydrochlorothiazid is potentially dangerous, increasing lithium’s nephrotoxicity and the thiazide diuretic's hepatotoxicity.

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