Upregulation of tissue factor expression by avastin: ex vivo and in vitro data

2012 ◽  
Vol 129 ◽  
pp. S170
Author(s):  
E. Napoleone ◽  
A. Cutrone ◽  
D. Cugino ◽  
R. Tambaro ◽  
A. De Curtis ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Ex Vivo ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Vitro Data ◽  
In Vitro Data

Tissue Factor Expression in Monocytes: In Vitro Compared to Ex Vivo

2000 ◽  
Vol 84 (09) ◽  
pp. 521-522 ◽  
Author(s):  
Bjarne Østerad
Keyword(s):  
Tissue Factor ◽  
Ex Vivo ◽  
Tissue Factor Expression ◽  
Factor Expression

Nutritional compounds influence tissue factor expression and inflammation of chronic kidney disease patients in vitro

Nutrition ◽  
2011 ◽  
Vol 27 (9) ◽  
pp. 967-972 ◽  
Author(s):  
Cecilia M. Shing ◽  
Murray J. Adams ◽  
Robert G. Fassett ◽  
Jeff S. Coombes
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Tissue Factor ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Nutritional Compounds

Stimulation of Tissue Factor Expression in Human Microvascular and Macrovascular Endothelial Cells by Cultured Vascular Smooth Muscle Cells in vitro

1999 ◽  
Vol 36 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Jing-Chuan Zhang ◽  
Qiu-Rong Ruan ◽  
Ljubomir Paucz ◽  
Astrid Fabry ◽  
Bernd R. Binder ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Tissue Factor ◽  
Vascular Smooth Muscle Cells ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Stimulation Of

scholarly journals Vascular Gas6 contributes to thrombogenesis and promotes tissue factor up-regulation after vessel injury in mice

Blood ◽  
2013 ◽  
Vol 121 (4) ◽  
pp. 692-699 ◽  
Author(s):  
Richard S. Robins ◽  
Catherine A. Lemarié ◽  
Sandrine Laurance ◽  
Meghedi N. Aghourian ◽  
Jianqiu Wu ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Thrombus Formation ◽  
Vascular Wall ◽  
Specific Gene ◽  
Tissue Factor Expression ◽  
Vessel Injury ◽  
Factor Expression ◽  
Platelet Signaling

Abstract Gas6 (growth-arrest specific gene 6) plays a role in thrombus stabilization. Gas6 null (−/−) mice are protected from lethal venous and arterial thromboembolism through platelet signaling defects induced only by 5μM ADP and 10μM of the thromboxane analog, U46619. This subtle platelet defect, despite a dramatic clinical phenotype, raises the possibility that Gas6 from a source other than platelets contributes to thrombus formation. Thus, we hypothesize that Gas6 derived from the vascular wall plays a role in venous thrombus formation. Bone marrow transplantation and platelet depletion/reconstitution experiments generating mice with selective ablations of Gas6 from either the hematopoietic or nonhematopoietic compartments demonstrate an approximately equal contribution by Gas6 from both compartments to thrombus formation. Tissue factor expression was significantly reduced in the vascular wall of Gas6−/− mice compared with WT. In vitro, thrombin-induced tissue factor expression was reduced in Gas6−/− endothelial cells compared with wild-type endothelium. Taken together, these results demonstrate that vascular Gas6 contributes to thrombus formation in vivo and can be explained by the ability of Gas6 to promote tissue factor expression and activity. These findings support the notion that vascular wall-derived Gas6 may play a pathophysiologic role in venous thromboembolism.

scholarly journals Stimulation of monocyte tissue factor expression in an in vitro model of bacterial endocarditis.

1994 ◽  
Vol 62 (12) ◽  
pp. 5669-5672 ◽  
Author(s):  
M J Bancsi ◽  
J Thompson ◽  
R M Bertina
Keyword(s):  
Tissue Factor ◽  
In Vitro Model ◽  
Bacterial Endocarditis ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Vitro Model ◽  
Stimulation Of

Abstract 337: Plasma Advanced-oxidized Protein Products Promote Platelet-endothelial Crosstalk and Endothelial Tissue Factor Expression

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Lisa Pasterk ◽  
Sandra Lemesch ◽  
Bettina Leber ◽  
Markus Trieb ◽  
Sanja Curcic ◽  
...  
Keyword(s):  
Renal Disease ◽  
Tissue Factor ◽  
Platelet Activation ◽  
Platelet Reactivity ◽  
Tissue Factor Expression ◽  
Serum Samples ◽  
Platelet Aggregometry ◽  
Factor Expression ◽  
Esrd Patients

Background/Aims: End-stage renal disease (ESRD) is associated with a prothrombotic phenotype and substantial activation of platelets can occur in the course of hemodialysis. However, the underlying mechanisms for increased platelet reactivity remain unclear. Increased levels of oxidized albumin, termed “advanced oxidation protein products (AOPPs)” accumulate in subjects with renal disease. Methods: Platelet aggregation was assessed with platelet aggregometry. Platelet activation, formation of reactive oxygen species and tissue factor expression on the surface of platelets and endothelial cells was measured by flow cytometry. Platelet adherence was assessed under flow using the Cellix system. The oxidative status of albumin isolated from ESRD patients was determined by photometric analysis. Tissue factor levels in serum samples were determined using a commercially available ELISA kit. Results: Albumin isolated from hemodialysis patients as well as in vitro generated AOPP-albumin promoted platelet activation via CD36, dependent on the AOPP-content of albumin. AOPP-albumin mediated platelet activation was prevented by scavenging superoxide anions and inhibitors of phospholipase C and protein kinase C. Furthermore, AOPP and serum tissue factor levels were considerably increased in ESRD patients on hemodialysis and a significant correlation of AOPP and serum tissue factor was found. Conclusion: Interaction of platelet CD36 with endogenous oxidized albumin may link oxidative stress with a prothrombotic phenotype in ESRD.

scholarly journals PF4/heparin-antibody complex induces monocyte tissue factor expression and release of tissue factor positive microparticles by activation of FcγRI

Blood ◽  
2012 ◽  
Vol 119 (22) ◽  
pp. 5285-5293 ◽  
Author(s):  
Raj S. Kasthuri ◽  
Sam L. Glover ◽  
William Jonas ◽  
Troy McEachron ◽  
Rafal Pawlinski ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Tissue Factor Expression ◽  
Drug Induced ◽  
Peripheral Blood Mononuclear ◽  
Platelet Factor ◽  
Heparin Induced Thrombocytopenia ◽  
Factor Expression ◽  
Antibody Complex

Heparin-induced thrombocytopenia (HIT) is a potentially devastating form of drug-induced thrombocytopenia that occurs in patients receiving heparin for prevention or treatment of thrombosis. Patients with HIT develop autoantibodies to the platelet factor 4 (PF4)/heparin complex, which is termed the HIT Ab complex. Despite a decrease in the platelet count, the most feared complication of HIT is thrombosis. The mechanism of thrombosis in HIT remains poorly understood. We investigated the effects of the HIT Ab complex on tissue factor (TF) expression and release of TF-positive microparticles in peripheral blood mononuclear cells and monocytes. To model these effects ex vivo, we used a murine mAb specific for the PF4/heparin complex (KKO), as well as plasma from patients with HIT. We found that the HIT Ab complex induced TF expression in monocytes and the release of TF-positive microparticles. Further, we found that induction of TF is mediated via engagement of the FcγRI receptor and activation of the MEK1-ERK1/2 signaling pathway. Our data suggest that monocyte TF may contribute to the development of thrombosis in patients with HIT.

Role of platelets in tissue factor expression by monocytes in normal and hypercholesterolemic subjects. In vitro effect of cerivastatin

2000 ◽  
Vol 30 (3) ◽  
pp. 147-156 ◽  
Author(s):  
L. Puccetti ◽  
F. Bruni ◽  
G. Bova ◽  
M. Cercignani ◽  
G. Pompella ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Vitro Effect ◽  
Hypercholesterolemic Subjects

VASOACTIVE INTESTINAL PEPTIDE AND PITUARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE INHIBIT TISSUE FACTOR EXPRESSION IN MONOCYTE IN VITRO AND IN VIVO

Shock ◽  
2009 ◽  
Vol 31 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Ben Lv ◽  
Yiting Tang ◽  
Fangping Chen ◽  
Xianzhong Xiao
Keyword(s):  
Adenylate Cyclase ◽  
Tissue Factor ◽  
Vasoactive Intestinal Peptide ◽  
Tissue Factor Expression ◽  
Factor Expression
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