Yeast mRNA Decapping Enzyme

2001 ◽  
pp. 226-233 ◽  
Author(s):  
Travis Dunckley ◽  
Roy Parker
Keyword(s):  
Mrna Decapping ◽  
Decapping Enzyme

The C. elegans mRNA decapping enzyme shapes morphology of cilia

2017 ◽  
Vol 493 (1) ◽  
pp. 382-387 ◽  
Author(s):  
Takeshi Adachi ◽  
Keigo Nagahama ◽  
Susumu Izumi
Keyword(s):  
Mrna Decapping ◽  
C Elegans ◽  
Decapping Enzyme

scholarly journals The hDcp2 protein is a mammalian mRNA decapping enzyme

2002 ◽  
Vol 99 (20) ◽  
pp. 12663-12668 ◽  
Author(s):  
Z. Wang ◽  
X. Jiao ◽  
A. Carr-Schmid ◽  
M. Kiledjian
Keyword(s):  
Mrna Decapping ◽  
Decapping Enzyme

scholarly journals Detection and Degradation of Nonsense-mediated mRNA Decay Substrates Involve Two Distinct Upf1-bound Complexes

2018 ◽  
Author(s):  
Marine Dehecq ◽  
Laurence Decourty ◽  
Abdelkader Namane ◽  
Caroline Proux ◽  
Joanne Kanaan ◽  
...  
Keyword(s):  
Large Scale ◽  
Mrna Decay ◽  
Affinity Purification ◽  
Rna Degradation ◽  
Degradation Pathway ◽  
Telomere Maintenance ◽  
Mrna Decapping ◽  
Nonsense Mediated Mrna Decay ◽  
Decapping Enzyme

AbstractNonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway involved in many cellular pathways and crucial for telomere maintenance and embryo development. Core NMD factors Upf1, Upf2 and Upf3 are conserved from yeast to mammals, but a universal NMD model is lacking. We used affinity purification coupled with mass spectrometry and an improved data analysis protocol to obtain the first large-scale quantitative characterization of yeast NMD complexes in yeast (112 experiments). Unexpectedly, we identified two distinct complexes associated with Upf1: Detector (Upf1/2/3) and Effector. Effector contained the mRNA decapping enzyme, together with Nmd4 and Ebs1, two proteins that globally affected NMD and were critical for RNA degradation mediated by the Upf1 C-terminal helicase region. The fact that Nmd4 association to RNA was dependent on Detector components and the similarity between Nmd4/Ebs1 and mammalian Smg5-7 proteins suggest that in all eukaryotes NMD operates through successive Upf1-bound Detector and Effector complexes.

scholarly journals miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation

2020 ◽  
Author(s):  
Qian Zhang ◽  
Yun-Fei Yan ◽  
Qing Lv ◽  
You-Jie Li ◽  
Ran-Ran Wang ◽  
...  
Keyword(s):  
Lung Carcinoma ◽  
Mrna Decapping ◽  
Lung Carcinoma Cells ◽  
Stat3 Phosphorylation ◽  
Rna Immunoprecipitation ◽  
Decapping Enzyme ◽  
Gain Loss ◽  
And Control ◽  
Relationship Of

Abstract Background: Emerging evidence shows that lncRNA WFDC21P could promote STAT3 phosphorylation and microRNA 4293 SNP is associated with the risk of carcinomas, but the oncogenic functions of WFDC21P and miR-4293 in lung carcinoma are unclear.Methods: mRNA sequencing of lung carcinoma and control para-carcinoma tissues was performed to screen the potential targets. WFDC21P and miR-4293 levels were evaluated in lung carcinoma cells and tissues by qRT-PCR. The function of WFDC21P and miR-4293 on proliferation, apoptosis and metastasis were assessed by MTT, FACS, western blot, transwell assays, colony formation assays and xenografts experiment. RNA immunoprecipitation assays were implemented to verify the relationship between WFDC21P and mRNA-decapping enzyme 2 (DCP2). Furthermore, gain/loss of miR-4293 functions were used to determine its targeting relationship of DCP2. Results: WFDC21P expression is markedly enhanced in lung carcinoma tissue and cells. Moreover, WFDC21P promotes tumor cell proliferation and metastasis but suppresses apoptosis. Mechanistic investigations identified DCP2 can directly bind to WFDC21P and down-regulates its expression. DCP2 as a direct target of miR-4293 and its expression is suppressed by miR-4293. Consequently, miR-4293 can further promote WFDC21P expression by regulating DCP2. With positive correlation to WFDC21P expression, miR-4293 also plays oncogenic role in lung carcinoma. Furthermore, knockdown of WFDC21P results in functional attenuation of miR-4293 on tumor promotion. In vivo xenograft growth is also promoted by both WFDC21P and miR-4293. Conclusion: Our results establish oncogenic roles for both WFDC21P and miR-4293, and demonstrate that interactions between miRNAs and lncRNAs through DCP2 are important in lung carcinoma pathogenesis.

scholarly journals Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome

2020 ◽  
Author(s):  
Isioma I.I. Enwerem ◽  
Nathan D. Elrod ◽  
Chung-Te Chang ◽  
Ai Lin ◽  
Ping Ji ◽  
...  
Keyword(s):  
Rna Binding ◽  
Rna Binding Proteins ◽  
Molecular Model ◽  
Direct Interaction ◽  
Vertebrate Development ◽  
Regulate Gene Expression ◽  
Mrna Decapping ◽  
Specific Mrnas ◽  
Decapping Enzyme ◽  
Pumilio Proteins

AbstractPumilio paralogs, PUM1 and PUM2, are sequence-specific RNA-binding proteins that are essential for vertebrate development and neurological functions. PUM1&2 negatively regulate gene expression by accelerating degradation of specific mRNAs. Here, we determined the repression mechanism and impact of human PUM1&2 on the transcriptome. We identified subunits of the CCR4-NOT (CNOT) deadenylase complex required for stable interaction with PUM1&2 and to elicit CNOT-dependent repression. Isoform-level RNA sequencing revealed broad co-regulation of target mRNAs through the PUM-CNOT repression mechanism.Functional dissection of the domains of PUM1&2 identified a conserved N-terminal region that confers the predominant repressive activity via direct interaction with CNOT. In addition, we show that the mRNA decapping enzyme, DCP2, has an important role in repression by PUM1&2 N-terminal regions. Our results support a molecular model of repression by human PUM1&2 via direct recruitment of CNOT deadenylation machinery in a decapping-dependent mRNA decay pathway.

scholarly journals General decapping activators target different subsets of inefficiently translated mRNAs

2018 ◽  
Author(s):  
Feng He ◽  
Alper Celik ◽  
Allan Jacobson
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Mrna Translation ◽  
Positive Regulation ◽  
Mrna Decapping ◽  
Terminal Domain ◽  
Genome Wide ◽  
Decapping Enzyme ◽  
Partial Overlap

AbstractThe Dcp1-Dcp2 decapping enzyme and the decapping activators Pat1, Dhh1, and Lsm1 regulate mRNA decapping, but their mechanistic integration is unknown. We analyzed the gene expression consequences of deleting PAT1, LSM1, or DHH1, or the DCP2 C-terminal domain, and found that: i) the Dcp2 C-terminal domain is an effector of both negative and positive regulation; ii) rather than being global activators of decapping, Pat1, Lsm1, and Dhh1 directly target specific subsets of yeast mRNAs and loss of the functions of each of these factors has substantial indirect consequences for genome-wide mRNA expression; and iii) transcripts targeted by Pat1, Lsm1, and Dhh1 exhibit only partial overlap, are generally translated inefficiently, and, as expected, are targeted to decapping-dependent decay. Our results define the roles of Pat1, Lsm1, and Dhh1 in decapping of general mRNAs and suggest that these factors may monitor mRNA translation and target unique features of individual mRNAs.

mRNA-decapping enzyme from Saccharomyces cerevisiae: purification and unique specificity for long RNA chains

1988 ◽  
Vol 8 (5) ◽  
pp. 2005-2010
Author(s):  
A Stevens
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rnase A ◽  
Micrococcal Nuclease ◽  
Mrna Decapping ◽  
Nuclease P1 ◽  
High Concentrations ◽  
Decapping Enzyme ◽  
Unique Specificity ◽  
End Group

An enzyme that hydrolyzes one PPi bond of the cap structure of mRNA, yielding m7GDP and 5'-p RNA was purified from Saccharomyces cerevisiae to a stage suitable for characterization. The specificity of the enzyme was studied, using both yeast mRNA and synthetic RNAs labeled in the cap structure. A synthetic capped RNA (540 nucleotides) was not reduced in size, while as much as 80% was decapped. Yeast mRNA treated with high concentrations of RNase A, nuclease P1, or micrococcal nuclease was inactive as a substrate. The use of synthetic capped RNAs of different sizes (50 to 540 nucleotides) as substrates showed that the larger RNA can be a better substrate by as much as 10-fold. GpppG-RNA was hydrolyzed at a rate similar to that at which 5'-triphosphate end group were not hydrolyzed.

Sign in / Sign up

Export Citation Format

Share Document