RADIOIMMUNOASSAY FOR HUMAN CHORIONIC GONADOTROPHIN AND LUTEINISING HORMONE

The Lancet ◽  
1966 ◽  
Vol 287 (7447) ◽  
pp. 1118-1121 ◽  
Author(s):  
K.D. Bagshawe ◽  
C.E. Wilde ◽  
ANNHILARY Orr
Keyword(s):  
Luteinising Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

scholarly journals The onset of nausea and vomiting of pregnancy: a prospective cohort study

2020 ◽  
Author(s):  
Roger Gadsby ◽  
Diana Ivanova ◽  
Emma Trevelyan ◽  
Jane Luise Hutton ◽  
Sarah Johnson
Keyword(s):  
Cohort Study ◽  
Luteinising Hormone ◽  
Outcome Measure ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Nausea And Vomiting ◽  
Daily Diaries ◽  
Main Outcome Measure ◽  
The United Kingdom ◽  
Time To Onset

Abstract BackgroundNausea and vomiting are experienced by most women during pregnancy. The onset is usually related to Last Menstrual Period (LMP) the date of which is often unreliable. This study describes the time to onset of nausea and vomiting symptoms from date of ovulation and compares this to date of last menstrual periodMethodsProspective cohort of women seeking to become pregnant, recruited from 12 May 2014 to 25 November 2016, in the United Kingdom. Daily diaries of nausea and vomiting were kept by 256 women who were trying to conceive. The main outcome measure is the number of days from last men­strual period (LMP) or luteinising hormone surge until onset of nausea or vomiting.ResultsAlmost all women (88\%) had Human Chorionic Gonadotrophin rise within 8 to 10 days of ovulation; the equivalent interval from LMP was 20 to 30 days. Many (67\%) women experience symp­toms within 11 to 20 days of ovulation.ConclusionsOnset of nausea and vomiting occurs earlier than previously reported and there is a narrow window for onset of symptoms. This indicates that its etiology is associated with a specific developmental stage at the fetal-maternal interface.Trial registrationNCT01577147

STUDIES ON THE SPECIFICITY OF THE RADIOIMMUNOASSAY OF LUTEINISING HORMONE

1970 ◽  
Vol 63 (3) ◽  
pp. 545-561 ◽  
Author(s):  
J. M. H. Buckler ◽  
Barbara E. Clayton
Keyword(s):  
Luteinising Hormone ◽  
Relative Concentration ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cross Reaction ◽  
The Cross ◽  
Stimulating Hormone

ABSTRACT The cross reaction of follicle stimulating hormone (FSH) with antisera raised to human chorionic gonadotrophin (HCG) for use in the radioimmunoassay of luteinising hormone (LH) has been found to be influenced by many factors. These include the relative concentration of the hormones, the concentration of antiserum, the length of the assay stages, and the particular preparation of FSH. Antisera do not react identically with HCG and LH. This effect varies from antiserum to antiserum and so does the ability to detect the immunological differences which become apparent between different preparations of FSH and between hormones of urinary and pituitary origin. These findings are of considerable relevance to the assessment of the validity of a radioimmunoassay system.

THE PRECISION OF THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1963 ◽  
Vol 43 (1) ◽  
pp. 155-160
Author(s):  
Jørgen Falck Larsen ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Clinical Analysis ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

ABSTRACT Various modifications of the Parlow test for luteinizing hormone (ovarian ascorbic acid depletion in rats) were tried. Human chorionic gonadotrophin was used instead of hypophyseal luteinizing hormone. The precision of the method was found to be so low, however, that the test could not be used for routine clinical analysis. The low precision found in this and other laboratories is thought to be due to the strains of rats used.

STUDIES ON THE AROMATISATION OF NEUTRAL STEROIDS IN PREGNANT WOMEN

1964 ◽  
Vol 45 (4) ◽  
pp. 535-559 ◽  
Author(s):  
E. Bolté ◽  
S. Mancuso ◽  
G. Eriksson ◽  
N. Wiqvist ◽  
E. Diczfalusy
Keyword(s):  
Pregnant Women ◽  
Comparative Studies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Radioactive Material ◽  
Placental Barrier ◽  
Therapeutic Abortion ◽  
Limited Ability ◽  
Better Than

ABSTRACT In 15 cases of therapeutic abortion by laparotomy the placenta was disconnected from the foetus and perfused in situ with tracer amounts of radioactive dehydroepiandrosterone (DHA), dehydroepiandrosterone sulphate (DHAS), androst-4-ene-3,17-dione (A), testosterone (T) and 17β-oestradiol (OE2). Analysis of the placentas, perfusates and urine samples revealed an extensive aromatisation of DHA, A and T; more than 70% of the radioactive material recovered was phenolic, and at least 80 % of this phenolic material was identified as oestrone (OE1), 17β-oestradiol (OE2) and oestriol (OE3), the latter being detected only in the urine. Comparative studies indicated that A and T were aromatised somewhat better than DHA and that all three unconjugated steroids were aromatised to a much greater extent than DHAS. Radioactive OE1 and OE2 were isolated and identified in the placentas and perfusates, but no OE3, epimeric oestriols, or ring D ketols could be detected in these sources, not even when human chorionic gonadotrophin (HCG) was added to the blood prior to perfusion. Lack of placental 16-hydroxylation was also apparent when OE2 was perfused. Regardless of the precursor perfused, there was three times more OE2 than OE1 in the placenta and three times more OE1 than OE2 in the perfusate. This was also the case following perfusion with OE2. The results are interpreted as suggesting the existence in the pregnant human of a placental »barrier« limiting the passage of circulating androgen. The barrier consists of a) limited ability to transfer directly DHAS and b) an enzymic mechanism resulting in the rapid and extensive aromatisation of the important androgens DHA, A and T.

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