THE PRECISION OF THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1963 ◽  
Vol 43 (1) ◽  
pp. 155-160
Author(s):  
Jørgen Falck Larsen ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Clinical Analysis ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

ABSTRACT Various modifications of the Parlow test for luteinizing hormone (ovarian ascorbic acid depletion in rats) were tried. Human chorionic gonadotrophin was used instead of hypophyseal luteinizing hormone. The precision of the method was found to be so low, however, that the test could not be used for routine clinical analysis. The low precision found in this and other laboratories is thought to be due to the strains of rats used.

SOME OBSERVATIONS ON THE OVARIAN ASCORBIC ACID DEPLETION METHOD AS A TEST FOR LUTEINIZING HORMONE ACTIVITY

1962 ◽  
Vol 23 (4) ◽  
pp. 413-421 ◽  
Author(s):  
H. SCHMIDT-ELMENDORFF ◽  
J. A. LORAINE
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Clinical Application ◽  
Sensitivity And Specificity ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Assay Method ◽  
Hormone Activity ◽  
Low Degree ◽  
High Degree

SUMMARY The assay method for luteinizing hormone (LH) activity depending on ovarian ascorbic acid depletion in rats (O.A.A.D. method) has been assessed in terms of its reliability criteria. The chief advantages of the method are its high degree of sensitivity and specificity and its good practicability. Its disadvantages are its relatively low degree of precision and the fact that a proportion of assays are invalid due to lack of parallelism. The LH activity of various gonadotrophin preparations has been investigated using the O.A.A.D. method. The LH content of NIH-FSH and of Pergonal was found to be low. The LH activity of pregnant mare's serum gonadotrophin was lower than that of human chorionic gonadotrophin. When purified urinary extracts are used, the O.A.A.D. method is suitable for clinical application.

THE EFFECT OF 6 m UREA ON THE FOLLICLE-STIMULATING AND LUTEINIZING HORMONE ACTIVITIES OF VARIOUS GONADOTROPHIN PREPARATIONS

1962 ◽  
Vol 24 (2) ◽  
pp. 153-158 ◽  
Author(s):  
H. SCHMIDT-ELMENDORFF ◽  
J. A. LORAINE ◽  
E. T. BELL
Keyword(s):  
Ascorbic Acid ◽  
Biological Activity ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Considerable Reduction ◽  
Mouse Uterus ◽  
Stimulating Hormone

SUMMARY The luteinizing hormone (LH), follicle-stimulating hormone (FSH) and 'total gonadotrophic' activities of various hormones have been studied following incubation with 6 m urea at 40° c for 24 hr. LH activity was estimated by the ovarian ascorbic acid depletion test in rats, FSH activity by the augmentation test in mice, and 'total gonadotrophic' activity by the mouse uterus test. Following incubation with 6 m urea the LH activities of NIH—LH, NIH—FSH, human chorionic gonadotrophin and Pergonal were almost completely destroyed, while the LH activity of pregnant mares' serum gonadotrophin (PMSG) was reduced to a smaller extent. The FSH activity of NIH—FSH was little affected by this form of treatment, but in the case of Pergonal a considerable reduction of FSH activity occurred. The 'total gonadotrophic' activity of NIH—FSH, PMSG and Pergonal was reduced after incubation with 6 m urea, the degree of inactivation being greatest in the case of Pergonal. After control incubations with water no appreciable loss of biological activity was observed with any hormone other than Pergonal.

ANTIGONADOTROPHIC PROFILE OF ANTISERA AGAINST HUMAN GONADOTROPHIN PREPARATIONS

1971 ◽  
Vol 67 (2) ◽  
pp. 262-276 ◽  
Author(s):  
P. Petrusz ◽  
C. Robyn ◽  
E. Diczfalusy
Keyword(s):  
Biological Activity ◽  
Luteinizing Hormone ◽  
Total Dose ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Immunological Activity ◽  
Human Menopausal Gonadotrophin ◽  
Stimulating Hormone

ABSTRACT Forty-two antisera were prepared in rabbits against human chorionic gonadotrophin (HCG), human hypophysial gonadotrophin (HHG), human urinary luteinizing hormone (LH) and human menopausal gonadotrophin (HMG) preparations. The gonadotrophic profiles of the antigens were previously characterized by bioassay, immunoassay and bioimmunoassay methods. The 25 most potent antisera were tested in statistically valid bioassays for their HCG and follicle stimulating hormone (FSH) neutralizing activities as well as for their neutralizing potencies against the FSH-like activity present in HCG preparations. The anti-HCG/anti-FSH ratios of the anti-HCG sera tested varied between 6.2 and > 254, while those of the anti-HHG, anti-LH and anti-HMG sera were close to 2. It was found that the total dose of immunological activity (anti-HCG neutralizing and anti-FSH neutralizing potency) rather than that of the biological activity administered to the rabbits was decisive for obtaining antisera with high anti-HCG and anti-FSH titers. Immunization with a highly purified HCG preparation (> 17 000 IU/mg) resulted in antisera exhibiting lower anti-HCG/anti-FSH ratios than did immunization with partially purified preparations. A highly purified urinary LH preparation which did not contain any detectable FSH activity gave rise to antisera exhibiting anti-HCG/anti-FSH ratios of approximately 2.0. These highly purified HCG and LH preparations were shown previously to possess high anti-FSH neutralizing potencies (Petrusz et al. 1971b). Booster injections did not change significantly the quality or the titer of the antigonadotrophic sera studied. The HCG neutralizing potency of anti-HCG sera was approximately 3 times higher when assayed against a highly purified HCG preparation (> 17 000 IU/mg) as compared to potency estimates obtained against the laboratory standard of HCG (about 2000 IU/mg). It is suggested that consideration should be given to the establishment of standard preparations of antigonadotrophic sera. It is concluded that bioimmunoassays are more suitably than conventional bioassay methods for the assessment of the antigenic purity of human gonadotrophin preparations.

INHIBITION BY MELATONIN OF THE PITUITARY RESPONSE TO LUTEINIZING HORMONE RELEASING HORMONE IN VIVO

1978 ◽  
Vol 76 (3) ◽  
pp. 487-491 ◽  
Author(s):  
K. YAMASHITA ◽  
M. MIENO ◽  
T. SHIMIZU ◽  
ER. YAMASHITA
Keyword(s):  
Luteinizing Hormone ◽  
Carotid Artery ◽  
Anterior Pituitary ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Lh Rh ◽  
Lh Secretion

The rate of secretion of 17-oxosteroids by the testes of anaesthetized dogs in vivo was used as an index of LH secretion. Intracarotid injection of luteinizing hormone releasing hormone (LH-RH, 1, 5 or 10 μg/kg body wt) resulted in an increase in the testicular 17-oxosteroid secretion which was roughly proportional to the dose administered and which reached a maximum 60 min after the injection. Testicular output of 17-oxosteroids was unaffected by administration of melatonin (10 or 100 μg/kg body wt) into the carotid artery. When LH-RH (5 μg/kg) was injected into the carotid artery 3 h after intracarotid injection of melatonin (10 or 100 μg/kg), the testicular response to LH-RH was considerably diminished. Pretreatment with melatonin (100 μg/kg) did not alter the testicular response to human chorionic gonadotrophin (20 i.u./kg body wt) given i.v. It is concluded that melatonin may act directly on the anterior pituitary gland in dogs to inhibit the LH-RH-induced release of LH.

Ovulation rate in ewes treated with pregnant mares' serum gonadotrophin and luteinizing hormone releasing factor

1976 ◽  
Vol 86 (1) ◽  
pp. 127-129 ◽  
Author(s):  
P. T. McGovern ◽  
J. A. Laing
Keyword(s):  
Luteinizing Hormone ◽  
Domestic Animals ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Ovulation Rate ◽  
Commercial Application ◽  
Established Procedure

Although the administration of pregnant mares' serum gonadotrophin (PMSG) to induce superovulation is a well established procedure (see Anderson, Schultz & Melampny, 1963), the unpredictability of the response to this treatment continues to impose a constraint on the commercial application of egg transfer techniques in the large domestic animals. In sheep, the additional use of human chorionic gonadotrophin (HCG) has been mainly centred on attempts to control the time of ovulation (Ortavant, Thibault & Wintenberger, 1949; Braden, Lamond & Radford, 1960; Dziuk et al. 1964; McGovern, Williams & Hancock, 1969). However, Killeen & Moore (1970) found that the proportion of follicles which rupture was increased in PMSG-treated ewes which had been given HCG at the beginning of oestrus. The purpose of the observations recorded here was to examine the possibility that a further gain in ovulation rate in PMSG-treated sheep might be obtained with the use of luteinizing hormone releasing factor (LHRF). No attempt was made to reproduce physiological levels of the releasing factor and dosage was aimed at achieving a superovulatory effect.

IMMUNOREACTIVE LUTEINIZING HORMONE AND PROGESTERONE DURING PREGNANCY AND FOLLOWING GONADOTROPHIN ADMINISTRATION IN BEAGLE BITCHES

1973 ◽  
Vol 72 (3) ◽  
pp. 573-581 ◽  
Author(s):  
Gwyneth E. Jones ◽  
A. R. Boyns ◽  
E. T. Bell ◽  
D. W. Christie ◽  
M. F. Parkes
Keyword(s):  
Luteinizing Hormone ◽  
Plasma Levels ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Pregnant Animal ◽  
Progesterone Secretion ◽  
Plasma Hormone

ABSTRACT Immunoreactive canine luteinizing hormone (IRCLH) and progesterone were measured in the plasma of Beagle bitches. Changes in plasma hormone concentrations during pregnancy were similar to those seen in the non-pregnant animal during metoestrus. Administration of pregnant mares' serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) to anoestrous bitches induced oestrus. However, the duration of progesterone secretion was shorter than that seen in pregnant bitches. Treatment appeared to stimulate the secretion of IRCLH and in some animals plasma levels reached a maximum some weeks after the end of oestrus.

PRODUCTION OF ANTISERA AGAINST HIGHLY PURIFIED HUMAN FOLLICLE-STIMULATING HORMONE, LUTEINIZING HORMONE AND THYROID-STIMULATING HORMONE

1976 ◽  
Vol 70 (3) ◽  
pp. 335-344 ◽  
Author(s):  
J. I. THORELL ◽  
B. HOLMSTRÖM
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
High Titre ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antigenic Determinants ◽  
High Avidity ◽  
Cross Reactions ◽  
Stimulating Hormone

SUMMARY Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7·5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the β-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.

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