Effect of isolation and cultivation procedures of trophoblastic cells of the human first-trimester placenta on the secretion of choriogonadotrophin (hCG)

Placenta ◽  
1986 ◽  
Vol 7 (5) ◽  
pp. 449
Author(s):  
C. Erlewein ◽  
W.E. Merz
Keyword(s):  
First Trimester ◽  
Trophoblastic Cells

scholarly journals Methods of detection and isolation of trophoblast cells from trans-cervical specimens – a historical overview

2021 ◽  
Vol 9 (4) ◽  
pp. 170-176
Author(s):  
Rafal Sibiak ◽  
Ewa Wender-Ożegowska
Keyword(s):  
Pregnancy Termination ◽  
First Trimester ◽  
Prenatal Diagnostics ◽  
Trophoblast Cells ◽  
Maternal Circulation ◽  
Fetal Dna ◽  
Trophoblastic Cells ◽  
Cervical Cell ◽  
Genetic Abnormalities ◽  
Planned Pregnancy

Abstract Trophoblast cells can be detected and isolated from the cervical epithelial cells obtained via various techniques of trans-cervical samples collection such as a mucus aspiration, endocervical lavage, or standard cervical brushing in the early first trimester, even from the 5 weeks’ gestation. Isolated fetal cells can be used in the early prediction of fetal sex, prenatal diagnostics of the most common aneuploidies, and any other genetic abnormalities. Nevertheless, the collection of trophoblastic cells has limited efficacy compared to currently used methods of detection of free fetal DNA in maternal circulation or other protocols of invasive prenatal diagnostics available at later stages of pregnancy. In the past years, trans-cervical cell samples were collected mainly in women before planned pregnancy termination. The early trophoblastic cells isolation from women in ongoing pregnancies opens new perspectives for further studies focused on the elucidation of pathophysiology of numerous pregnancy-related complications.

scholarly journals Accumulation of malto-oligosaccharides in the syncytiotrophoblastic cells of first-trimester human placentas

1981 ◽  
Vol 200 (1) ◽  
pp. 93-98 ◽  
Author(s):  
S J Fisher ◽  
R A Laine
Keyword(s):  
Gel Filtration ◽  
First Trimester ◽  
Structural Elements ◽  
Trophoblastic Cells ◽  
Highly Active ◽  
A Cell ◽  
Liver Homogenates ◽  
High Concentrations ◽  
Very High

A cell-surface microvillar fraction that was isolated from the syncytiotrophoblastic cells of first-trimester human placentas was found to contain very high concentrations (890 +/- 32 microgram of hexose/mg of protein) of a class of low-molecular-weight oligosaccharides that were comprised entirely of glucose. T.l.c. and gel filtration showed that the saccharides contained from one to six glucose residues. The structures of the most prominent members of the series, a tetra- and a tri-saccharide, were determined. The anomeric configuration of the glucose residues was alpha, and methylation linkage analysis gave terminal and 4-linked hexose residues. These malto-oligosaccharides contained one reducing terminus per molecule, indicating that they were free and not bound to other structural elements of the cells. Within the placenta they appeared to be concentrated in the first-trimester trophoblastic cells, since crude membrane and particulate fractions isolated from either term trophoblastic cells or cultured placental fibroblasts did not contain detectable amounts of glucose oligomers. This series of oligosaccharides was similar to the products that are formed when glycogen is degraded by alpha-amylase in liver homogenates and may be indicative of a similar, highly active enzymic reaction closely associated with the brush border of the syncytiotrophoblastic cells of the first-trimester human placenta. Although the role of these oligosaccharides remains obscure they are probably involved in foetal metabolism.

scholarly journals Characterization and differentiation of human first trimester placenta trophoblastic cells in culture

1990 ◽  
Vol 30 (2) ◽  
pp. 183-192 ◽  
Author(s):  
M. Dodeur ◽  
A. Malassine ◽  
D. Bellet ◽  
A. Mensier ◽  
D. Evain-Brion
Keyword(s):  
First Trimester ◽  
Cells In Culture ◽  
Trophoblastic Cells

Effect of parathyroid hormone on the endocrine functions of human trophoblastic cells of first trimester placenta

Placenta ◽  
1989 ◽  
Vol 10 (5) ◽  
pp. 502 ◽  
Author(s):  
D. Evain-Brion ◽  
M. Dodeur ◽  
A. Mensier ◽  
E. Alsat ◽  
J.M. Bidarta
Keyword(s):  
Parathyroid Hormone ◽  
First Trimester ◽  
Trophoblastic Cells

scholarly journals The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line

2021 ◽  
Vol 22 (19) ◽  
pp. 10200
Author(s):  
Agata Sakowicz ◽  
Michalina Bralewska ◽  
Tadeusz Pietrucha ◽  
Francesc Figueras ◽  
Dominika E. Habrowska-Górczyńska ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Line ◽  
Critical Role ◽  
First Trimester ◽  
Trophoblastic Cell ◽  
Trophoblastic Cells ◽  
Hypoxic Conditions ◽  
Activation Of Transcription ◽  
Placental Cells

Preeclampsia is a pregnancy disorder associated with shallow placentation, forcing placental cells to live in hypoxic conditions. This activates the transcription factor kappa B (NFκB) in maternal and placental cells. Although the role of NFκB in preeclampsia is well documented, its mechanism of activation in trophoblastic cells has been never studied. This study investigates the mechanism of NFκB activation in a first trimester trophoblastic cell line (HTR8/SVneo) stimulated by a medium containing serum from preeclamptic (PE) or normotensive (C) women in hypoxic (2% O2) or normoxic (8% O2) conditions. The results indicate that in HTR8/SVneo cells, the most widely studied NFκB pathways, i.e., canonical, non-canonical and atypical, are downregulated in environment PE 2% O2 in comparison to C 8% O2. Therefore, other pathways may be responsible for NFκB activation. One such pathway depends on the activation of NFκB by the p53/RSK1 complex through its phosphorylation at Serine 536 (pNFκB Ser536). The data generated by our study show that inhibition of the p53/RSK1 pathway by p53-targeted siRNA results in a depletion of pNFκB Ser536 in the nucleus, but only in cells incubated with PE serum at 2% O2. Thus, the p53/RSK1 complex might play a critical role in the activation of NFκB in trophoblastic cells and preeclamptic placentas.

scholarly journals An Incidental Ultrasonographic Diagnosis of Partial Hydatidiform Mole in a Old Primigravida: A Case Report

2020 ◽  
Vol 58 (222) ◽  
Author(s):  
Sudeep Thapa ◽  
Ramesh Rana ◽  
Sheela Kumari
Keyword(s):  
Hydatidiform Mole ◽  
Cardiac Activity ◽  
First Trimester ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antenatal Visit ◽  
Partial Hydatidiform Mole ◽  
Trophoblastic Cells ◽  
Asian Populations ◽  
Beta Human Chorionic Gonadotrophin

Hydatidiform mole is an abnormal pregnancy common in Asian populations compared to western countries; however, a partial hydatidiform mole is relatively uncommon and very challenging to diagnose on ultrasound. We reported a 24 years old female visited our clinic whose first antenatal visit was regular with normal viable fetus on ultrasonographic scan. However, an uneventful scan at 12 weeks period of gestation revealed a large irregular gestational sac with the disproportionately small embryo corresponding to 6+6 weeks with no cardiac activity. Additionally, there was markedly thickened placenta measuring 30mm in thickness without cystic spaces within the placenta. Further, her beta-human chorionic gonadotrophin level was very high and suction evacuation sample showed hydropic chorionic villi lined by trophoblastic cells with cistern formation and scalloped border. In conclusion, partial hydatidiform mole is an uncommon molar pregnancy rarely diagnose on ultrasonography. Late first-trimester scan and excessively high beta-human chorionic gonadotrophin levels confirm the diagnosis.

Degradation of extracellular matrix by the trophoblastic cells of first-trimester human placentas

1985 ◽  
Vol 27 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Susan J. Fisher ◽  
Mark S. Leitch ◽  
Marsha S. Kantor ◽  
Carol B. Basbaum ◽  
Randall H. Kramer
Keyword(s):  
Extracellular Matrix ◽  
First Trimester ◽  
Trophoblastic Cells

scholarly journals Detection of Fetal Aneuploidies by QF-PCR in Transcervical Cell Samples

ISRN Genetics ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Riccardo Cioni ◽  
Cecilia Bussani ◽  
Mariarosaria Di Tommaso
Keyword(s):  
Tandem Repeats ◽  
Dna Analysis ◽  
Pcr Amplification ◽  
First Trimester ◽  
Pcr Analysis ◽  
Trophoblastic Cells ◽  
Standard Karyotype ◽  
Polymerase Chain ◽  
Fluorescent Polymerase Chain Reaction ◽  
Fetal Aneuploidies

Objective. To evaluate the accuracy in the diagnosis of aneuploidies of a quantitative fluorescent polymerase chain reaction (QF-PCR) assay on trophoblastic cells recovered from transcervical cells samples (TCCs) collected by intrauterine lavage (IUL). Study Design. DNA analysis was performed on cells of seemingly trophoblastic origin isolated from IUL samples collected prior to first trimester termination of pregnancy. The analysis was performed by multiplex QF-PCR, using a panel of 29 polymorphic short tandem repeats (STRs) for the chromosomes X, Y, 21, 13, and 18. Results. The QF-PCR analysis on placental samples revealed that among the three cases studied there were two cases of trisomy 21 and one case of monosomy X; the comparison of peak profiles obtained from IUL, placental, and maternal samples confirmed the diagnosis of aneuploidy in all three cases. Conclusion. This study suggests that the detection of chromosomal aneuploidies in micromanipulated TCC samples can be achieved by QF-PCR amplification of selected highly polymorphic and chromosome specific markers. With respect to standard karyotype, QF-PCR analysis has the limitation that only numerical abnormalities of selected chromosomes can be detected but retains the advantages of being quicker, less expensive, and less lab demanding.

71 WHAT IS THE UTERINE RESPONSE IN A CLONED BOVINE PREGNANCY?

2006 ◽  
Vol 18 (2) ◽  
pp. 144 ◽  
Author(s):  
T. C. Santos ◽  
F. T. V. Pereira ◽  
A. C. Assis Neto ◽  
C. E. Ambrósio ◽  
F. V. Meirelles ◽  
...  
Keyword(s):  
Cell Cycle ◽  
First Trimester ◽  
Giant Cells ◽  
Differential Analysis ◽  
Placental Development ◽  
Trophoblastic Cells ◽  
M Phase ◽  
Cell Cycle Phases ◽  
Cloned Bovine ◽  
Trophoblast Giant Cells

Bovine has a synepitheliochorial placenta and characteristically there is no invasion of the trophoblast, but there is migration of the binucleate trophoblast giant cells into the maternal endometrium. The feto-maternal interface occurs in placentome where a tridimensional organization permits interactions between maternal epithelium and trophoblast, and in the intercaruncular area it is possible to observe a few mini-placentomes and the uterine glands opening. The objective of the present investigation was to study the morphological aspects of the uterus in bovine that had a cloned cattle gestations to understand the differences with natural gestation. The uterus and fetal membranes from natural and cloned cattle gestations were collected, fixed in 10% formaldehyde, processed, and stained for light microscopy and immunohistochemistry. The morphological differences observed in the surrogate uterus were: extensive areas without placentome, hemorrhagic uterine areas, caruncular fusion giving a reduced number of caruncules, increase in size and weight (megacaruncules), and a significant number of mini-caruncules giving miniplacentomes (diameter < 1 cm). In particular the mini-placentome showed functional trophoblastic cells with PAS+ granules in the binucleate trophoblast giant cells and an intense subepithelial capillary organization in maternal and fetal sides. The normal and clone placentomal cell populations were analyzed throughout pregnancy. The population of tetraploid and diploid trophoblastic cells was stained; detached cell cycle and DNA content was measured in FL2 using a FACscalibur flow cytometric system. We determined the percentage of cells in apoptosis (sub-G1), quiescent cells (G0/G1), synthesis (S), and proliferative cells (G2/M) with the aid of ModFit software. In addition, a cell cycle differential analysis was performed, and the tetraploid population presented statistical differences in cell cycle phases and populations relative to the apoptosis rate for the first (7.5 � 3.1%), second (15.2 � 5.0%) and third (17.3 � 4.3%) trimesters. The number of apoptotic cells increased significantly during pregnancy stages. The results showed that first trimester presented the majority of its cells in the G0-G1 phase, starting the cell cycle. On the other hand, the second and third trimesters presented the majority of their cells in the G2-M phase, ending the cell cycle. The relationship between cell cycle phases/rate of apoptosis in mononucleate cells, days of normal and cloned pregnancy, the number of binucleate cells, and their metabolic activity as well as their developmental kinetics could be important data in several studies that involve placental development in natural pregnancy or that derived from laboratory-manipulated embryos. This work was supported by FAPESP and CNPq.

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