Differential display analysis of oxygen-mediated changes in gene expression in first trimester human trophoblast cells

Placenta ◽  
1998 ◽  
Vol 19 (7) ◽  
pp. 483-488 ◽  
Author(s):  
B.J. Pak ◽  
H. Park ◽  
E.R. Chang ◽  
S.C. Pang ◽  
C.H. Graham
Keyword(s):  
Gene Expression ◽  
Differential Display ◽  
First Trimester ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Differential Display Analysis ◽  
Human Trophoblast Cells

scholarly journals Kidney-Replenishing Herb Induces SOCS-3 Expression via ERK/MAPK Pathway and Improves Growth of the First-Trimester Human Trophoblast Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-12
Author(s):  
Chang-ying Xing ◽  
De-xiang Zhang ◽  
Sui-qi Gui ◽  
Min-fang Tao
Keyword(s):  
Mapk Pathway ◽  
Recurrent Miscarriage ◽  
First Trimester ◽  
Mek Inhibitor ◽  
Biological Functions ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Mitogen Activated Protein ◽  
Human Trophoblast Cells

Kidney-replenishing herb is a traditional medicine formula in China which has been widely used for clinical treatment of recurrent miscarriage. Our previous study showed that Kidney-replenishing herb could promote proliferation and inhibit apoptosis of the human first-trimester trophoblasts. In the present study, we further explored the potential mechanism and signal pathway of Kidney-replenishing herb on human trophoblast cells. Our research showed that Kidney-replenishing herb stimulated proliferation and reduced apoptosis of human trophoblast cells in vitro, and this appeared to be positive correlation with SOCS-3 transcription, suggesting that Kidney-replenishing herb regulated biological functions of human trophoblast cells by inducing SCOS-3 expression. Furthermore, the Kidney-replenishing herb treatment stimulated the phosphorylation of ERK1/2, and blocking the signaling pathway by mitogen-activated protein MAPK (MEK) inhibitor, U0126, inhibited Kidney-replenishing herb-induced SOCS-3 transcription, depressed proliferation, and promoted apoptosis of human trophoblasts. Kidney-replenishing herbs still induced ERK1/2 phosphorylation after SOCS-3 siRNA silence. Overexpression of SOCS-3 stimulated the proliferation of trophoblast. These findings suggest that SOCS-3 expression is induced by Kidney-replenishing herbs via activation of MAPK pathways, and this may possibly be involved in promoting human trophoblast cells growth which is contributed to embryo development.

Immunoregulatory activity in supernatants from cultures of normal human trophoblast cells of the first trimester

1989 ◽  
Vol 161 (2) ◽  
pp. 446-453 ◽  
Author(s):  
Mrinal K. Sanyal ◽  
Charles J. Brami ◽  
Paul Bischof ◽  
Edwina Simmons ◽  
Eytan R. Barnea ◽  
...  
Keyword(s):  
First Trimester ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Normal Human ◽  
Human Trophoblast Cells

scholarly journals Kisspeptin Regulation of Genes Involved in Cell Invasion and Angiogenesis in First Trimester Human Trophoblast Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99680 ◽  
Author(s):  
Víctor A. Francis ◽  
Aron B. Abera ◽  
Mushi Matjila ◽  
Robert P. Millar ◽  
Arieh A. Katz
Keyword(s):  
Cell Invasion ◽  
First Trimester ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Human Trophoblast Cells

scholarly journals Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells

Placenta ◽  
2011 ◽  
Vol 32 (1) ◽  
pp. 33-43 ◽  
Author(s):  
R. Apps ◽  
A. Sharkey ◽  
L. Gardner ◽  
V. Male ◽  
M. Trotter ◽  
...  
Keyword(s):  
Expression Profile ◽  
First Trimester ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Genome Wide ◽  
Genome Wide Expression ◽  
Human Trophoblast Cells

scholarly journals Peroxisome Proliferator-Activated Receptor δ Suppresses 11β-Hydroxysteroid Dehydrogenase Type 2 Gene Expression in Human Placental Trophoblast Cells

Endocrinology ◽  
2005 ◽  
Vol 146 (3) ◽  
pp. 1482-1490 ◽  
Author(s):  
Laura Julan ◽  
Haiyan Guan ◽  
Jonathan P. van Beek ◽  
Kaiping Yang
Keyword(s):  
Gene Expression ◽  
Promoter Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Peroxisome Proliferator ◽  
Trophoblast Cells ◽  
Peroxisome Proliferator Activated Receptor ◽  
Human Trophoblast ◽  
Placental Function ◽  
Human Trophoblast Cells

Accumulating evidence suggests that the human placental enzyme 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) plays a key role in fetal development by controlling fetal exposure to maternal glucocorticoids. Recently, the nuclear peroxisome proliferator-activated receptor δ (PPARδ) has been found to be the most abundantly expressed PPAR subtype in the human placenta, but its function in this organ is unknown. Given that PPARδ-null mice exhibited placental defects and consequent intrauterine growth restriction, the present study was undertaken to examine the hypothesis that PPARδ regulates human placental function in part by targeting 11β-HSD2. Using cultured human trophoblast cells as a model system, we demonstrated that 1) the putative PPARδ agonist carbaprostacyclin (cPGI2) reduced 11β-HSD2 activity as well as 11β-HSD2 expression at both protein and mRNA levels; 2) GW610742 (a selective PPARδ agonist) mimicked the effect of cPGI2, whereas indomethacin (a known ligand for PPARα and PPARγ) had no effect; 3) the cPGI2-induced down-regulation of 11β-HSD2 mRNA did not require de novo protein synthesis; 4) cPGI2 suppressed HSD11B2 promoter activity, but did not alter the half-life of 11β-HSD2 mRNA; and 5) the inhibitory effect of cPGI2 on HSD11B2 promoter activity was abrogated in trophoblast cells cotransfected with a dominant negative PPARδ mutant. Taken together, these findings suggest that activation of PPARδ down-regulates HSD11B2 gene expression in human trophoblast cells, and that this effect is mediated primarily at the transcriptional level. Thus, the present study reveals 11β-HSD2 as an additional target for PPARδ and identifies a molecular mechanism by which this nuclear receptor may regulate human placental function.

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