Size exclusion chromatography as a tool for quality control of recombinant allergens and hypoallergenic variants

2003 ◽  
Vol 56 (1-3) ◽  
pp. 219-232 ◽  
Author(s):  
Bernhard Weber ◽  
Holger Slamal ◽  
Roland Suck
Keyword(s):  
Quality Control ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Recombinant Allergens ◽  
Exclusion Chromatography

Size exclusion chromatography for the characterization and quality control of biologics

2021 ◽  
pp. 1-14
Author(s):  
Surinder M. Singh ◽  
Ran Furman ◽  
Rajesh K. Singh ◽  
Gurusamy Balakrishnan ◽  
Naresh Chennamsetty ◽  
...  
Keyword(s):  
Quality Control ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography

scholarly journals Quality control considerations for size exclusion chromatography with online ICP-MS: a powerful tool for evaluating the size dependence of metal–organic matter complexation

2013 ◽  
Vol 67 (5) ◽  
pp. 1075-1082 ◽  
Author(s):  
Erica R. McKenzie ◽  
Thomas M. Young
Keyword(s):  
Quality Control ◽  
Organic Matter ◽  
Size Exclusion Chromatography ◽  
Inductively Coupled Plasma ◽  
Control Measures ◽  
Size Exclusion ◽  
Molecular Volume ◽  
Peak Time ◽  
Icp Ms ◽  
Exclusion Chromatography

Size exclusion chromatography (SEC), which separates molecules based on molecular volume, can be coupled with online inductively coupled plasma mass spectrometry (ICP-MS) to explore size-dependent metal–natural organic matter (NOM) complexation. To make effective use of this analytical dual detector system, the operator should be mindful of quality control measures. Al, Cr, Fe, Se, and Sn all exhibited columnless attenuation, which indicated unintended interactions with system components. Based on signal-to-noise ratio and peak reproducibility between duplicate analyses of environmental samples, consistent peak time and height were observed for Mg, Cl, Mn, Cu, Br, and Pb. Al, V, Fe, Co, Ni, Zn, Se, Cd, Sn, and Sb were less consistent overall, but produced consistent measurements in select samples. Ultrafiltering and centrifuging produced similar peak distributions, but glass fiber filtration produced more high molecular weight (MW) peaks. Storage in glass also produced more high MW peaks than did plastic bottles.

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